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BLUEPRINT: Epigenetic programming during monocyte to macrophage differentiation and trained innate immunity

Monocyte differentiation into macrophages represents a cornerstone process for host defense. Concomitantly, immunological imprinting of either tolerance or trained immunity determines the functional fate of macrophages and susceptibility to secondary infections. Transcriptomes (RNA-Seq) and epigenomes (ChIP-Seq H3K4me1,H3K4me3,H3K27ac) in four primary cell types: monocytes, in vitro differentiated naive, tolerized and trained macrophages were characterized. Inflammatory and metabolic pathways were modulated in macrophages, including decreased inflammasome activation, and pathways functionally implicated in trained immunity were identified. Strikingly, B-glucan training elicits an exclusive epigenetic signature, revealing a complex network of enhancers and promoters. Analysis of transcription factor motifs in DNase I hypersensitive sites at cell-type specific epigenetic loci unveiled differentiation and treatment specific repertoires. Altogether, this study provides a resource to understand the epigenetic changes that underlie innate immunity in humans.

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Blueprint Data Access Policy

Access to the sequence and alignment and genotype level data produced by the Blueprint Consortium is controlled by the Blueprint Data Access Committee (DAC). Access to data will be granted to qualified investigators for appropriate use. Please email to request data access.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001000326 Epigenetics
EGAS00001000951 Other
EGAS00001000953 Other
EGAS00001000954 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00000148969 fastq.gz 4.6 GB
EGAF00000148978 fastq.gz 1.7 GB
EGAF00000148980 fastq.gz 2.6 GB
EGAF00000154479 fastq.gz 1.3 GB
EGAF00000154480 fastq.gz 1.4 GB
EGAF00000154481 fastq.gz 1.6 GB
EGAF00000161836 fastq.gz 594.3 MB
EGAF00000161840 fastq.gz 2.5 GB
EGAF00000161849 fastq.gz 2.0 GB
EGAF00000161861 fastq.gz 1.6 GB
EGAF00000161871 fastq.gz 1.2 GB
EGAF00000161872 fastq.gz 1.5 GB
EGAF00000161876 fastq.gz 1.3 GB
EGAF00000178954 fastq.gz 1.8 GB
EGAF00000239248 fastq.gz 1.7 GB
EGAF00000239250 fastq.gz 1.8 GB
EGAF00000239253 fastq.gz 1.9 GB
EGAF00000239255 fastq.gz 1.7 GB
EGAF00000239256 fastq.gz 1.4 GB
EGAF00000239261 fastq.gz 2.5 GB
EGAF00000239265 fastq.gz 1.7 GB
EGAF00000239270 fastq.gz 1.5 GB
EGAF00000239278 fastq.gz 1.5 GB
EGAF00000239280 fastq.gz 1.5 GB
EGAF00000239281 fastq.gz 1.6 GB
EGAF00000239282 fastq.gz 1.6 GB
EGAF00000239284 fastq.gz 1.8 GB
EGAF00000239300 fastq.gz 2.3 GB
EGAF00000239302 fastq.gz 1.4 GB
EGAF00000239306 fastq.gz 1.3 GB
EGAF00000239318 fastq.gz 1.7 GB
EGAF00000239321 fastq.gz 1.5 GB
EGAF00000239326 fastq.gz 1.8 GB
EGAF00000239333 fastq.gz 1.6 GB
EGAF00000284324 fastq.gz 1.3 GB
EGAF00000284330 fastq.gz 1.2 GB
EGAF00000284341 fastq.gz 1.8 GB
EGAF00000284350 fastq.gz 1.5 GB
EGAF00000507555 fastq.gz 1.3 GB
EGAF00000507570 fastq.gz 1.7 GB
EGAF00000507589 fastq.gz 1.4 GB
EGAF00000507599 fastq.gz 724.9 MB
EGAF00000507606 fastq.gz 1.5 GB
EGAF00000604456 fastq.gz 1.2 GB
EGAF00000604457 fastq.gz 1.1 GB
EGAF00000604458 fastq.gz 910.8 MB
EGAF00000604459 fastq.gz 1.2 GB
EGAF00000604460 fastq.gz 896.2 MB
EGAF00000604461 fastq.gz 822.5 MB
EGAF00000604462 fastq.gz 967.8 MB
EGAF00000604463 fastq.gz 948.7 MB
EGAF00000604464 fastq.gz 1.0 GB
EGAF00000604465 fastq.gz 874.6 MB
EGAF00000604466 fastq.gz 1.2 GB
EGAF00000604467 fastq.gz 1.0 GB
EGAF00000604468 fastq.gz 1.4 GB
EGAF00000604469 fastq.gz 1.7 GB
EGAF00000604470 fastq.gz 1.1 GB
EGAF00000604471 fastq.gz 1.8 GB
EGAF00000604472 fastq.gz 1.4 GB
EGAF00000604473 fastq.gz 724.6 MB
EGAF00000604474 fastq.gz 953.8 MB
EGAF00000604475 fastq.gz 1.5 GB
EGAF00000604476 fastq.gz 840.9 MB
EGAF00000604477 fastq.gz 1.2 GB
EGAF00000604478 fastq.gz 2.1 GB
EGAF00000604479 fastq.gz 1.4 GB
EGAF00000604553 fastq.gz 1.2 GB
EGAF00000604554 fastq.gz 1.9 GB
EGAF00000604583 fastq.gz 1.5 GB
EGAF00000604858 fastq.gz 3.0 GB
EGAF00000604859 fastq.gz 3.4 GB
EGAF00000604860 fastq.gz 940.6 MB
EGAF00000604861 fastq.gz 915.1 MB
EGAF00000604862 fastq.gz 1.1 GB
EGAF00000604863 fastq.gz 1.1 GB
EGAF00000604864 fastq.gz 1.4 GB
EGAF00000604865 fastq.gz 989.7 MB
EGAF00000604866 fastq.gz 1.1 GB
EGAF00000604867 fastq.gz 1.3 GB
EGAF00000604868 fastq.gz 1.1 GB
EGAF00000604869 fastq.gz 966.1 MB
EGAF00000604870 fastq.gz 1.7 GB
EGAF00000604871 fastq.gz 3.5 GB
EGAF00000604872 fastq.gz 1.1 GB
EGAF00000604873 fastq.gz 1.1 GB
EGAF00000604874 fastq.gz 3.2 GB
EGAF00000604875 fastq.gz 1.3 GB
EGAF00000604876 fastq.gz 1.3 GB
EGAF00000604877 fastq.gz 1.3 GB
EGAF00000605474 fastq.gz 1.7 GB
EGAF00000605475 fastq.gz 1.5 GB
EGAF00000605476 fastq.gz 1.6 GB
EGAF00000605477 fastq.gz 1.5 GB
EGAF00000605478 fastq.gz 1.2 GB
EGAF00000605479 fastq.gz 1.1 GB
EGAF00000605480 fastq.gz 801.3 MB
EGAF00000605481 fastq.gz 919.2 MB
EGAF00000605482 fastq.gz 963.4 MB
EGAF00000605483 fastq.gz 851.6 MB
EGAF00000605484 fastq.gz 930.1 MB
EGAF00000605485 fastq.gz 836.2 MB
EGAF00000605486 fastq.gz 1.8 GB
EGAF00000605487 fastq.gz 1.8 GB
EGAF00000605488 fastq.gz 1.6 GB
EGAF00000605489 fastq.gz 1.7 GB
EGAF00000605490 fastq.gz 2.4 GB
EGAF00000605491 fastq.gz 1.2 GB
EGAF00000605492 fastq.gz 872.0 MB
EGAF00000605493 fastq.gz 1.9 GB
EGAF00000613714 fastq.gz 1.5 GB
111 Files (164.7 GB)