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Comparison of transcriptional response of induced pluripotent stem (iPS) cell-derived and monocyte-derived macrophages to bacterial lipopolysaccharide stimulation

In the first part of this project, we will differentiate IPS cells from 5 human donors into macrophages, and extract RNA from unstimulated and LPS stimulated macrophages to perform RNA sequencing. We will also extract RNA before and after stimulation in blood- derived macrophages from 5 additional, unrelated healthy samples. In the second part of the project, RNA-seq data will be analysed to compare LPS response of these two macrophage populations. In summary, we will perform 75bp PE RNA-seq on 20 samples (10 pre and post stimulus), on the HiSeq 2500 platform. Samples will be multiplexed at 5 samples / lane, so we will require 4 flow cells in total.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see

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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001000563 Transcriptome Analysis

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00000287475 bam 1.8 GB
EGAF00000287476 bam 1.6 GB
EGAF00000287477 bam 3.7 GB
EGAF00000287478 bam 2.0 GB
EGAF00000287479 bam 2.1 GB
EGAF00000287480 bam 4.2 GB
EGAF00000287481 bam 3.6 GB
EGAF00000287482 bam 2.5 GB
EGAF00000287483 bam 1.8 GB
EGAF00000287484 bam 1.6 GB
EGAF00000287485 bam 3.7 GB
EGAF00000287486 bam 2.0 GB
EGAF00000287487 bam 2.1 GB
EGAF00000287488 bam 4.2 GB
EGAF00000287489 bam 3.6 GB
EGAF00000287490 bam 2.5 GB
EGAF00000519041 cram 1.0 GB
EGAF00000519042 cram 1.2 GB
EGAF00000519043 cram 1.3 GB
EGAF00000519044 cram 1.2 GB
EGAF00000519045 cram 1.3 GB
EGAF00000519046 cram 1.2 GB
EGAF00000519047 cram 1.2 GB
EGAF00000519048 cram 1.2 GB
EGAF00000519049 cram 968.1 MB
EGAF00000519050 cram 1.2 GB
EGAF00000519051 cram 999.9 MB
EGAF00000519052 cram 1.2 GB
EGAF00000519053 cram 1.2 GB
EGAF00000519054 cram 1.1 GB
EGAF00000519055 cram 1.2 GB
EGAF00000519056 cram 1.2 GB
EGAF00000519057 cram 1.1 GB
EGAF00000519058 cram 1.2 GB
EGAF00000519059 cram 946.9 MB
EGAF00000519060 cram 1.2 GB
36 Files (66.0 GB)