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MINCR is a MYC-induced lncRNA able to modulate MYC’s transcriptional network in Burkitt lymphoma cells

Despite the established role of the transcription factor MYC in cancer, little is known about the impact of a new class of transcriptional regulators, the long non-coding RNAs (lncRNAs), on the way MYC is able to influence cellular transcriptome. To this aim we have intersected RNA-sequencing data from two MYC-inducible cell lines and from a cohort of 91 mature B-cell lymphomas carrying, or not carrying, genetic variants resulting in MYC over-expression. By this approach, we identified 13 lncRNAs differentially expressed in IG-MYC-positive Burkitt lymphoma and regulated in the same direction by MYC in the model cell lines. Among them we focused on a lncRNA that we named MINCR, for MYC-Induced long Non-Coding RNA, showing a strong correlation with MYC expression in MYC-positive lymphomas and also in pancreatic ductal adenocarcinomas. To understand its cellular role we performed RNA interference (RNAi) experiments and found that MINCR knock-down is associated with a reduction in cellular viability, due to an impairment in cell cycle progression. Differential gene expression analysis following RNAi showed a strongly significant enrichment of cell cycle genes among the genes down-regulate following MINCR knock-down. Interestingly these genes are enriched in MYC binding sites in their promoters, suggesting that MINCR acts as a modulator of MYC transcriptional program. Accordingly, following MINCR knock-down, we observed a reduction in the binding of MYC to the promoters of selected cell cycle genes. Finally we provide evidences that down-regulation of AURKA, AURKB and CTD1 may explain the reduction in cellular proliferation observed upon MINCR knock-down. We therefore suggest that MINCR is a newly identified player in the MYC transcriptional network able to control the expression of cell cycle genes.

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ICGC Data Access

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001001199 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00000780147 fastq.gz 8.7 GB
EGAF00000780148 fastq.gz 8.9 GB
EGAF00000780229 fastq.gz 8.6 GB
EGAF00000780230 fastq.gz 8.7 GB
EGAF00000780231 fastq.gz 7.1 GB
EGAF00000780232 fastq.gz 7.1 GB
EGAF00000780420 fastq.gz 7.3 GB
EGAF00000780421 fastq.gz 7.4 GB
EGAF00000780422 fastq.gz 9.5 GB
EGAF00000780423 fastq.gz 9.7 GB
EGAF00000780424 fastq.gz 8.0 GB
EGAF00000780425 fastq.gz 8.2 GB
EGAF00000780426 fastq.gz 11.3 GB
EGAF00000780427 fastq.gz 11.5 GB
EGAF00000780428 fastq.gz 7.7 GB
EGAF00000780429 fastq.gz 7.8 GB
EGAF00000780430 fastq.gz 7.8 GB
EGAF00000780431 fastq.gz 8.0 GB
EGAF00000780432 fastq.gz 8.5 GB
EGAF00000780433 fastq.gz 8.7 GB
EGAF00000780434 fastq.gz 7.5 GB
EGAF00000780435 fastq.gz 7.7 GB
EGAF00000780436 fastq.gz 7.3 GB
EGAF00000780437 fastq.gz 7.5 GB
EGAF00000780438 fastq.gz 6.8 GB
EGAF00000780439 fastq.gz 6.9 GB
EGAF00000780440 fastq.gz 6.7 GB
EGAF00000780441 fastq.gz 6.8 GB
EGAF00000780442 fastq.gz 8.3 GB
EGAF00000780443 fastq.gz 8.5 GB
EGAF00000780444 fastq.gz 5.2 GB
EGAF00000780445 fastq.gz 5.3 GB
EGAF00000780446 fastq.gz 6.9 GB
EGAF00000780447 fastq.gz 6.9 GB
EGAF00000780448 fastq.gz 6.0 GB
EGAF00000780449 fastq.gz 6.1 GB
EGAF00000780450 fastq.gz 7.2 GB
EGAF00000780451 fastq.gz 7.1 GB
EGAF00000780452 fastq.gz 4.8 GB
EGAF00000780453 fastq.gz 4.9 GB
EGAF00000780454 fastq.gz 9.9 GB
EGAF00000780455 fastq.gz 9.8 GB
EGAF00000780456 fastq.gz 5.5 GB
EGAF00000780457 fastq.gz 5.5 GB
EGAF00000780458 fastq.gz 5.6 GB
EGAF00000780459 fastq.gz 5.6 GB
EGAF00000780460 fastq.gz 1.8 GB
EGAF00000780461 fastq.gz 1.8 GB
EGAF00000780462 fastq.gz 3.1 GB
EGAF00000780463 fastq.gz 3.2 GB
EGAF00000780464 fastq.gz 3.9 GB
EGAF00000780465 fastq.gz 4.0 GB
EGAF00000780466 fastq.gz 4.6 GB
EGAF00000780467 fastq.gz 4.7 GB
EGAF00000780468 fastq.gz 3.1 GB
EGAF00000780469 fastq.gz 3.2 GB
EGAF00000780470 fastq.gz 2.8 GB
EGAF00000780471 fastq.gz 2.8 GB
EGAF00000780472 fastq.gz 3.7 GB
EGAF00000780473 fastq.gz 3.7 GB
EGAF00000780474 fastq.gz 3.9 GB
EGAF00000780475 fastq.gz 4.0 GB
EGAF00000780476 fastq.gz 3.8 GB
EGAF00000780477 fastq.gz 3.9 GB
EGAF00000780478 fastq.gz 4.6 GB
EGAF00000780479 fastq.gz 4.6 GB
EGAF00000780480 fastq.gz 3.6 GB
EGAF00000780481 fastq.gz 3.7 GB
EGAF00000780482 fastq.gz 3.2 GB
EGAF00000780483 fastq.gz 3.2 GB
EGAF00000780484 fastq.gz 8.5 GB
EGAF00000780485 fastq.gz 8.5 GB
EGAF00000780486 fastq.gz 7.4 GB
EGAF00000780487 fastq.gz 7.3 GB
EGAF00000780488 fastq.gz 8.2 GB
EGAF00000780489 fastq.gz 8.2 GB
EGAF00000780490 fastq.gz 8.6 GB
EGAF00000780491 fastq.gz 8.6 GB
EGAF00000780492 fastq.gz 7.3 GB
EGAF00000780493 fastq.gz 7.3 GB
EGAF00000780494 fastq.gz 7.7 GB
EGAF00000780495 fastq.gz 7.7 GB
EGAF00000780496 fastq.gz 2.7 GB
EGAF00000780497 fastq.gz 2.8 GB
EGAF00000780498 fastq.gz 3.5 GB
EGAF00000780499 fastq.gz 3.5 GB
EGAF00000780500 fastq.gz 2.9 GB
EGAF00000780501 fastq.gz 3.0 GB
EGAF00000780502 fastq.gz 4.0 GB
EGAF00000780503 fastq.gz 4.1 GB
EGAF00000780504 fastq.gz 3.7 GB
EGAF00000780505 fastq.gz 3.7 GB
EGAF00000780506 fastq.gz 6.7 GB
EGAF00000780507 fastq.gz 6.7 GB
EGAF00000780508 fastq.gz 7.4 GB
EGAF00000780509 fastq.gz 7.5 GB
EGAF00000780510 fastq.gz 7.0 GB
EGAF00000780511 fastq.gz 7.0 GB
98 Files (602.6 GB)