Need Help?

Amplicon-based sequencing of drug resistant organoids

A BRAF V600E colorectal organoid which is sensitive to MAP kinase inhibition was mutagenised with the chemical mutagen ENU and then drug selected using a combination of Trametinib, Dabrafenib and Cetuximab. Single cell derived organoids were then manually picked and expanded in drug. Resistance was confirmed in a 14 day assay and DNA was collected. These then underwent targeted amplicon-based sequencing to confirm candidate resistance effectors from a screen in 2 2D BRAF V600E colorectal cell lines. Pools of resistant clones were also sequenced.

Request Access

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001001639 Cancer Genomics

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00001303303 cram 13.4 MB
EGAF00001303304 cram 17.8 MB
EGAF00001303305 cram 13.5 MB
EGAF00001303306 cram 11.0 MB
EGAF00001303307 cram 12.4 MB
EGAF00001303308 cram 17.6 MB
EGAF00001303309 cram 23.4 MB
EGAF00001303310 cram 17.3 MB
EGAF00001303311 cram 11.5 MB
EGAF00001303312 cram 12.8 MB
EGAF00001303313 cram 16.2 MB
EGAF00001303314 cram 12.9 MB
EGAF00001303315 cram 10.7 MB
EGAF00001303316 cram 11.9 MB
EGAF00001303317 cram 13.2 MB
EGAF00001303318 cram 12.5 MB
EGAF00001303319 cram 15.1 MB
EGAF00001303320 cram 14.7 MB
EGAF00001303321 cram 10.7 MB
EGAF00001303322 cram 6.9 MB
EGAF00001303323 cram 13.6 MB
EGAF00001303324 cram 17.5 MB
EGAF00001303325 cram 10.9 MB
EGAF00001303326 cram 13.6 MB
EGAF00001303327 cram 15.6 MB
EGAF00001303328 cram 10.8 MB
EGAF00001303329 cram 20.2 MB
EGAF00001303330 cram 12.3 MB
EGAF00001303331 cram 13.6 MB
EGAF00001303332 cram 16.8 MB
EGAF00001303333 cram 9.3 MB
EGAF00001303334 cram 11.0 MB
EGAF00001303335 cram 37.1 MB
EGAF00001303336 cram 26.4 MB
EGAF00001303337 cram 9.8 MB
EGAF00001303338 cram 27.9 MB
36 Files (542.0 MB)