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FASTQ files of total RNA-Seq data of POPS control samples

Placental biopsies (n = 64 female placentas, n = 67 male placentas) were selected from healthy pregnancies from the POPs cohort. These patients had no evidence of hypertension at booking and during pregnancy, did not experience pre-eclampsia, Hemolysis, Elevated Liver enzymes, and Low Platelets (HELLP) syndrome, gestational diabetes, or diabetes mellitus type I or type II and other obstetric complications. They delivered live babies with a birth weight percentile in the normal range (20-80th percentile), with no evidence of slowing in fetal growth trajectory. Chorionic villi from the corresponding placentas (free from decidua, visible infarction, calcification, hematoma, or damage) were collected and processed within 30 minutes of separation from the uterus. After repeated washes in chilled phosphate buffered saline, the samples were placed in RNA later (Applied Biosystems) and stored at -80°C. Total placental RNA was extracted using mirVana Isolation Kit (Ambion). For each placenta, approximately 5 mg of tissue were homogenized in the Lysis/Binding solution for 20 sec at 6 m/s using a bead beater (FastPrep24) and Lysing Matrix D Tubes (MP Biomedicals). The samples were then spun at 13,000 rpm for 5 min at 4°C and the supernatants recovered. Afterwards, the manufacturer's instructions were followed. Immediately after the RNA extraction, placental RNA samples were DNase-treated using DNA-free DNA Removal Kit (Ambion), aliquoted, and stored in -80°C. Quantity and quality of the RNA samples were assessed using the Agilent 2100 Bioanalyzer, the Agilent RNA 6000 Nano Kit (Agilent Technologies), and Qubit fluorometer. Libraries were prepared starting with 300-500 ng of good quality total RNA (RIN ≥7.5) using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat (Illumina), according to the manufacturer's instructions. The kit contains 96 uniquely indexed adapter combinations in order to allow pooling of multiple samples prior to sequencing. After determining their size (with the Agilent 2100 Bioanalyzer and the Agilent High Sensitivity DNA Kit by Agilent Technologies) and concentration (by qPCR with the KAPA Illumina ABI Prism Library Quantification Kit, Kapa Biosystems), libraries have been pooled and sequenced (single-end, 125 bp) using a Single End V4 Cluster Kit and an Illumina HiSeq2500 or HiSeq4000 instrument.

Request Access

DUO:0000006
version: 2019-01-07

health or medical or biomedical research

This data use permission indicates that use is allowed for health/medical/biomedical purposes; does not include the study of population origins or ancestry.

DUO:0000026
version: 2019-01-07

user specific restriction

This data use modifier indicates that use is limited to use by approved users.

POPS data access policy

You are not authroised to access POPS data unless you have a written permission from the data access committee which you can contact below: D. Stephen Charnock-Jones. Professor of Reproductive Biology. Department of Obstetrics and Gynaecology. University of Cambridge. The Rosie Hospital, Cambridge. CB2 0SW UK email dscj1@cam.ac.uk Tel +44 1223 336875 Fax +44 1223 215327

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001002205 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
EGAF00001704053 fq.gz 1.0 GB Report
EGAF00001704054 fq.gz 737.3 MB Report
EGAF00001704055 fq.gz 1.1 GB Report
EGAF00001704056 fq.gz 1.1 GB Report
EGAF00001704057 fq.gz 1.0 GB Report
EGAF00001704058 fq.gz 1.2 GB Report
EGAF00001704059 fq.gz 1.0 GB Report
EGAF00001704060 fq.gz 1.0 GB Report
EGAF00001704061 fq.gz 1.5 GB Report
EGAF00001704062 fq.gz 1.1 GB Report
EGAF00001704063 fq.gz 1.1 GB Report
EGAF00001704064 fq.gz 1.1 GB Report
EGAF00001704065 fq.gz 741.4 MB Report
EGAF00001704066 fq.gz 869.2 MB Report
EGAF00001704067 fq.gz 849.9 MB Report
EGAF00001704068 fq.gz 859.0 MB Report
EGAF00001704069 fq.gz 902.8 MB Report
EGAF00001704070 fq.gz 906.2 MB Report
EGAF00001704071 fq.gz 869.9 MB Report
EGAF00001704072 fq.gz 787.1 MB Report
EGAF00001704073 fq.gz 775.1 MB Report
EGAF00001704074 fq.gz 731.3 MB Report
EGAF00001704075 fq.gz 777.7 MB Report
EGAF00001704076 fq.gz 795.6 MB Report
EGAF00001704077 fq.gz 631.2 MB Report
EGAF00001704078 fq.gz 655.2 MB Report
EGAF00001704079 fq.gz 627.0 MB Report
EGAF00001704080 fq.gz 693.0 MB Report
EGAF00001704081 fq.gz 826.9 MB Report
EGAF00001704082 fq.gz 820.2 MB Report
EGAF00001704083 fq.gz 809.4 MB Report
EGAF00001704084 fq.gz 642.8 MB Report
EGAF00001704085 fq.gz 691.5 MB Report
EGAF00001704086 fq.gz 711.1 MB Report
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EGAF00001704088 fq.gz 573.3 MB Report
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EGAF00001704189 fq.gz 702.3 MB Report
EGAF00001704190 fq.gz 1.0 GB Report
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EGAF00001704192 fq.gz 982.0 MB Report
EGAF00001704193 fq.gz 1.1 GB Report
EGAF00001704194 fq.gz 893.8 MB Report
EGAF00001704195 fq.gz 891.1 MB Report
EGAF00001704196 fq.gz 1.3 GB Report
EGAF00001704197 fq.gz 955.4 MB Report
EGAF00001704198 fq.gz 953.1 MB Report
EGAF00001704199 fq.gz 914.5 MB Report
EGAF00001704200 fq.gz 764.8 MB Report
EGAF00001704201 fq.gz 904.7 MB Report
EGAF00001704202 fq.gz 818.6 MB Report
EGAF00001704203 fq.gz 835.8 MB Report
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EGAF00001704211 fq.gz 540.2 MB Report
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EGAF00001704218 fq.gz 703.9 MB Report
EGAF00001704219 fq.gz 752.2 MB Report
EGAF00001704220 fq.gz 764.4 MB Report
EGAF00001704221 fq.gz 612.5 MB Report
EGAF00001704222 fq.gz 634.6 MB Report
EGAF00001704223 fq.gz 609.5 MB Report
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EGAF00001704225 fq.gz 492.0 MB Report
EGAF00001704226 fq.gz 456.4 MB Report
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EGAF00001704234 fq.gz 418.9 MB Report
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EGAF00001704291 fq.gz 343.4 MB Report
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