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FASTQ files of total RNA-Seq data of POPS control samples

Placental biopsies (n = 64 female placentas, n = 67 male placentas) were selected from healthy pregnancies from the POPs cohort. These patients had no evidence of hypertension at booking and during pregnancy, did not experience pre-eclampsia, Hemolysis, Elevated Liver enzymes, and Low Platelets (HELLP) syndrome, gestational diabetes, or diabetes mellitus type I or type II and other obstetric complications. They delivered live babies with a birth weight percentile in the normal range (20-80th percentile), with no evidence of slowing in fetal growth trajectory. Chorionic villi from the corresponding placentas (free from decidua, visible infarction, calcification, hematoma, or damage) were collected and processed within 30 minutes of separation from the uterus. After repeated washes in chilled phosphate buffered saline, the samples were placed in RNA later (Applied Biosystems) and stored at -80°C. Total placental RNA was extracted using mirVana Isolation Kit (Ambion). For each placenta, approximately 5 mg of tissue were homogenized in the Lysis/Binding solution for 20 sec at 6 m/s using a bead beater (FastPrep24) and Lysing Matrix D Tubes (MP Biomedicals). The samples were then spun at 13,000 rpm for 5 min at 4°C and the supernatants recovered. Afterwards, the manufacturer's instructions were followed. Immediately after the RNA extraction, placental RNA samples were DNase-treated using DNA-free DNA Removal Kit (Ambion), aliquoted, and stored in -80°C. Quantity and quality of the RNA samples were assessed using the Agilent 2100 Bioanalyzer, the Agilent RNA 6000 Nano Kit (Agilent Technologies), and Qubit fluorometer. Libraries were prepared starting with 300-500 ng of good quality total RNA (RIN ≥7.5) using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat (Illumina), according to the manufacturer's instructions. The kit contains 96 uniquely indexed adapter combinations in order to allow pooling of multiple samples prior to sequencing. After determining their size (with the Agilent 2100 Bioanalyzer and the Agilent High Sensitivity DNA Kit by Agilent Technologies) and concentration (by qPCR with the KAPA Illumina ABI Prism Library Quantification Kit, Kapa Biosystems), libraries have been pooled and sequenced (single-end, 125 bp) using a Single End V4 Cluster Kit and an Illumina HiSeq2500 or HiSeq4000 instrument.

Request Access

POPS data access policy

You are not authroised to access POPS data unless you have a written permission from the data access committee which you can contact below: D. Stephen Charnock-Jones. Professor of Reproductive Biology. Department of Obstetrics and Gynaecology. University of Cambridge. The Rosie Hospital, Cambridge. CB2 0SW UK email dscj1@cam.ac.uk Tel +44 1223 336875 Fax +44 1223 215327

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001002205 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00001704053 fq.gz 1.0 GB
EGAF00001704054 fq.gz 737.3 MB
EGAF00001704055 fq.gz 1.1 GB
EGAF00001704056 fq.gz 1.1 GB
EGAF00001704057 fq.gz 1.0 GB
EGAF00001704058 fq.gz 1.2 GB
EGAF00001704059 fq.gz 1.0 GB
EGAF00001704060 fq.gz 1.0 GB
EGAF00001704061 fq.gz 1.5 GB
EGAF00001704062 fq.gz 1.1 GB
EGAF00001704063 fq.gz 1.1 GB
EGAF00001704064 fq.gz 1.1 GB
EGAF00001704065 fq.gz 741.4 MB
EGAF00001704066 fq.gz 869.2 MB
EGAF00001704067 fq.gz 849.9 MB
EGAF00001704068 fq.gz 859.0 MB
EGAF00001704069 fq.gz 902.8 MB
EGAF00001704070 fq.gz 906.2 MB
EGAF00001704071 fq.gz 869.9 MB
EGAF00001704072 fq.gz 787.1 MB
EGAF00001704073 fq.gz 775.1 MB
EGAF00001704074 fq.gz 731.3 MB
EGAF00001704075 fq.gz 777.7 MB
EGAF00001704076 fq.gz 795.6 MB
EGAF00001704077 fq.gz 631.2 MB
EGAF00001704078 fq.gz 655.2 MB
EGAF00001704079 fq.gz 627.0 MB
EGAF00001704080 fq.gz 693.0 MB
EGAF00001704081 fq.gz 826.9 MB
EGAF00001704082 fq.gz 820.2 MB
EGAF00001704083 fq.gz 809.4 MB
EGAF00001704084 fq.gz 642.8 MB
EGAF00001704085 fq.gz 691.5 MB
EGAF00001704086 fq.gz 711.1 MB
EGAF00001704087 fq.gz 552.8 MB
EGAF00001704088 fq.gz 573.3 MB
EGAF00001704089 fq.gz 552.0 MB
EGAF00001704090 fq.gz 612.8 MB
EGAF00001704091 fq.gz 424.5 MB
EGAF00001704092 fq.gz 425.5 MB
EGAF00001704093 fq.gz 421.2 MB
EGAF00001704094 fq.gz 424.0 MB
EGAF00001704095 fq.gz 424.2 MB
EGAF00001704096 fq.gz 392.9 MB
EGAF00001704097 fq.gz 411.0 MB
EGAF00001704098 fq.gz 410.2 MB
EGAF00001704099 fq.gz 409.8 MB
EGAF00001704100 fq.gz 409.4 MB
EGAF00001704101 fq.gz 409.7 MB
EGAF00001704102 fq.gz 409.4 MB
EGAF00001704103 fq.gz 409.7 MB
EGAF00001704104 fq.gz 382.5 MB
EGAF00001704105 fq.gz 488.1 MB
EGAF00001704106 fq.gz 485.8 MB
EGAF00001704107 fq.gz 484.0 MB
EGAF00001704108 fq.gz 487.1 MB
EGAF00001704109 fq.gz 486.9 MB
EGAF00001704110 fq.gz 431.0 MB
EGAF00001704111 fq.gz 433.6 MB
EGAF00001704112 fq.gz 434.6 MB
EGAF00001704113 fq.gz 438.5 MB
EGAF00001704114 fq.gz 439.0 MB
EGAF00001704115 fq.gz 408.0 MB
EGAF00001704116 fq.gz 401.0 MB
EGAF00001704117 fq.gz 400.9 MB
EGAF00001704118 fq.gz 406.6 MB
EGAF00001704119 fq.gz 407.9 MB
EGAF00001704120 fq.gz 401.3 MB
EGAF00001704121 fq.gz 403.6 MB
EGAF00001704122 fq.gz 405.1 MB
EGAF00001704123 fq.gz 376.8 MB
EGAF00001704124 fq.gz 327.7 MB
EGAF00001704125 fq.gz 327.1 MB
EGAF00001704126 fq.gz 327.2 MB
EGAF00001704127 fq.gz 326.4 MB
EGAF00001704128 fq.gz 327.0 MB
EGAF00001704129 fq.gz 444.7 MB
EGAF00001704130 fq.gz 502.2 MB
EGAF00001704131 fq.gz 489.3 MB
EGAF00001704132 fq.gz 485.3 MB
EGAF00001704133 fq.gz 471.5 MB
EGAF00001704134 fq.gz 484.0 MB
EGAF00001704135 fq.gz 486.3 MB
EGAF00001704136 fq.gz 487.9 MB
EGAF00001704137 fq.gz 462.5 MB
EGAF00001704138 fq.gz 498.4 MB
EGAF00001704139 fq.gz 490.1 MB
EGAF00001704140 fq.gz 489.5 MB
EGAF00001704141 fq.gz 486.7 MB
EGAF00001704142 fq.gz 482.4 MB
EGAF00001704143 fq.gz 490.0 MB
EGAF00001704144 fq.gz 485.9 MB
EGAF00001704145 fq.gz 463.7 MB
EGAF00001704146 fq.gz 386.6 MB
EGAF00001704147 fq.gz 387.4 MB
EGAF00001704148 fq.gz 365.6 MB
EGAF00001704149 fq.gz 360.7 MB
EGAF00001704150 fq.gz 360.7 MB
EGAF00001704151 fq.gz 361.4 MB
EGAF00001704152 fq.gz 361.9 MB
EGAF00001704153 fq.gz 359.9 MB
EGAF00001704154 fq.gz 362.1 MB
EGAF00001704155 fq.gz 361.2 MB
EGAF00001704156 fq.gz 337.5 MB
EGAF00001704157 fq.gz 402.9 MB
EGAF00001704158 fq.gz 400.5 MB
EGAF00001704159 fq.gz 400.5 MB
EGAF00001704160 fq.gz 390.9 MB
EGAF00001704161 fq.gz 399.8 MB
EGAF00001704162 fq.gz 401.2 MB
EGAF00001704163 fq.gz 400.9 MB
EGAF00001704164 fq.gz 372.7 MB
EGAF00001704165 fq.gz 382.8 MB
EGAF00001704166 fq.gz 380.0 MB
EGAF00001704167 fq.gz 344.7 MB
EGAF00001704168 fq.gz 353.3 MB
EGAF00001704169 fq.gz 351.4 MB
EGAF00001704170 fq.gz 353.0 MB
EGAF00001704171 fq.gz 353.5 MB
EGAF00001704172 fq.gz 351.6 MB
EGAF00001704173 fq.gz 357.0 MB
EGAF00001704174 fq.gz 352.8 MB
EGAF00001704175 fq.gz 333.3 MB
EGAF00001704176 fq.gz 324.5 MB
EGAF00001704177 fq.gz 325.3 MB
EGAF00001704178 fq.gz 326.2 MB
EGAF00001704179 fq.gz 326.1 MB
EGAF00001704180 fq.gz 324.4 MB
EGAF00001704181 fq.gz 326.5 MB
EGAF00001704182 fq.gz 325.8 MB
EGAF00001704183 fq.gz 303.7 MB
EGAF00001704184 fq.gz 363.6 MB
EGAF00001704185 fq.gz 361.9 MB
EGAF00001704186 fq.gz 600.2 MB
EGAF00001704187 fq.gz 972.6 MB
EGAF00001704188 fq.gz 951.4 MB
EGAF00001704189 fq.gz 702.3 MB
EGAF00001704190 fq.gz 1.0 GB
EGAF00001704191 fq.gz 1.0 GB
EGAF00001704192 fq.gz 982.0 MB
EGAF00001704193 fq.gz 1.1 GB
EGAF00001704194 fq.gz 893.8 MB
EGAF00001704195 fq.gz 891.1 MB
EGAF00001704196 fq.gz 1.3 GB
EGAF00001704197 fq.gz 955.4 MB
EGAF00001704198 fq.gz 953.1 MB
EGAF00001704199 fq.gz 914.5 MB
EGAF00001704200 fq.gz 764.8 MB
EGAF00001704201 fq.gz 904.7 MB
EGAF00001704202 fq.gz 818.6 MB
EGAF00001704203 fq.gz 835.8 MB
EGAF00001704204 fq.gz 891.0 MB
EGAF00001704205 fq.gz 753.0 MB
EGAF00001704206 fq.gz 747.0 MB
EGAF00001704207 fq.gz 736.9 MB
EGAF00001704208 fq.gz 637.1 MB
EGAF00001704209 fq.gz 664.5 MB
EGAF00001704210 fq.gz 678.2 MB
EGAF00001704211 fq.gz 540.2 MB
EGAF00001704212 fq.gz 559.6 MB
EGAF00001704213 fq.gz 536.3 MB
EGAF00001704214 fq.gz 591.3 MB
EGAF00001704215 fq.gz 683.3 MB
EGAF00001704216 fq.gz 680.8 MB
EGAF00001704217 fq.gz 670.7 MB
EGAF00001704218 fq.gz 703.9 MB
EGAF00001704219 fq.gz 752.2 MB
EGAF00001704220 fq.gz 764.4 MB
EGAF00001704221 fq.gz 612.5 MB
EGAF00001704222 fq.gz 634.6 MB
EGAF00001704223 fq.gz 609.5 MB
EGAF00001704224 fq.gz 491.5 MB
EGAF00001704225 fq.gz 492.0 MB
EGAF00001704226 fq.gz 456.4 MB
EGAF00001704227 fq.gz 445.8 MB
EGAF00001704228 fq.gz 446.4 MB
EGAF00001704229 fq.gz 452.7 MB
EGAF00001704230 fq.gz 454.0 MB
EGAF00001704231 fq.gz 449.3 MB
EGAF00001704232 fq.gz 451.4 MB
EGAF00001704233 fq.gz 452.1 MB
EGAF00001704234 fq.gz 418.9 MB
EGAF00001704235 fq.gz 336.9 MB
EGAF00001704236 fq.gz 336.6 MB
EGAF00001704237 fq.gz 336.5 MB
EGAF00001704238 fq.gz 336.5 MB
EGAF00001704239 fq.gz 336.6 MB
EGAF00001704240 fq.gz 335.8 MB
EGAF00001704241 fq.gz 336.9 MB
EGAF00001704242 fq.gz 313.7 MB
EGAF00001704243 fq.gz 388.3 MB
EGAF00001704244 fq.gz 388.0 MB
EGAF00001704245 fq.gz 385.9 MB
EGAF00001704246 fq.gz 388.5 MB
EGAF00001704247 fq.gz 388.2 MB
EGAF00001704248 fq.gz 362.1 MB
EGAF00001704249 fq.gz 419.9 MB
EGAF00001704250 fq.gz 412.5 MB
EGAF00001704251 fq.gz 410.7 MB
EGAF00001704252 fq.gz 400.2 MB
EGAF00001704253 fq.gz 409.2 MB
EGAF00001704254 fq.gz 411.4 MB
EGAF00001704255 fq.gz 412.3 MB
EGAF00001704256 fq.gz 387.1 MB
EGAF00001704257 fq.gz 408.4 MB
EGAF00001704258 fq.gz 403.6 MB
EGAF00001704259 fq.gz 403.1 MB
EGAF00001704260 fq.gz 402.5 MB
EGAF00001704261 fq.gz 399.9 MB
EGAF00001704262 fq.gz 381.5 MB
EGAF00001704263 fq.gz 381.9 MB
EGAF00001704264 fq.gz 379.7 MB
EGAF00001704265 fq.gz 385.8 MB
EGAF00001704266 fq.gz 381.8 MB
EGAF00001704267 fq.gz 360.3 MB
EGAF00001704268 fq.gz 315.2 MB
EGAF00001704269 fq.gz 317.0 MB
EGAF00001704270 fq.gz 317.9 MB
EGAF00001704271 fq.gz 318.1 MB
EGAF00001704272 fq.gz 316.2 MB
EGAF00001704273 fq.gz 318.4 MB
EGAF00001704274 fq.gz 317.3 MB
EGAF00001704275 fq.gz 295.9 MB
EGAF00001704276 fq.gz 350.5 MB
EGAF00001704277 fq.gz 347.7 MB
EGAF00001704278 fq.gz 347.4 MB
EGAF00001704279 fq.gz 339.0 MB
EGAF00001704280 fq.gz 346.9 MB
EGAF00001704281 fq.gz 362.0 MB
EGAF00001704282 fq.gz 355.1 MB
EGAF00001704283 fq.gz 361.9 MB
EGAF00001704284 fq.gz 362.5 MB
EGAF00001704285 fq.gz 362.0 MB
EGAF00001704286 fq.gz 336.2 MB
EGAF00001704287 fq.gz 346.2 MB
EGAF00001704288 fq.gz 343.5 MB
EGAF00001704289 fq.gz 345.1 MB
EGAF00001704290 fq.gz 345.6 MB
EGAF00001704291 fq.gz 343.4 MB
EGAF00001704292 fq.gz 348.7 MB
EGAF00001704293 fq.gz 345.3 MB
EGAF00001704294 fq.gz 325.6 MB
EGAF00001704295 fq.gz 322.6 MB
EGAF00001704296 fq.gz 324.4 MB
EGAF00001704297 fq.gz 325.1 MB
EGAF00001704298 fq.gz 325.3 MB
EGAF00001704299 fq.gz 323.0 MB
EGAF00001704300 fq.gz 891.1 MB
EGAF00001704301 fq.gz 889.8 MB
EGAF00001704302 fq.gz 879.7 MB
EGAF00001704303 fq.gz 901.4 MB
EGAF00001704304 fq.gz 527.4 MB
EGAF00001704305 fq.gz 880.2 MB
EGAF00001704306 fq.gz 781.4 MB
EGAF00001704307 fq.gz 588.8 MB
EGAF00001704308 fq.gz 866.1 MB
EGAF00001704309 fq.gz 865.2 MB
EGAF00001704310 fq.gz 865.3 MB
EGAF00001704311 fq.gz 855.5 MB
EGAF00001704312 fq.gz 865.6 MB
EGAF00001704313 fq.gz 576.9 MB
EGAF00001704314 fq.gz 1.1 GB
EGAF00001704315 fq.gz 1.0 GB
EGAF00001704316 fq.gz 640.3 MB
EGAF00001704317 fq.gz 1.1 GB
EGAF00001704318 fq.gz 1.1 GB
EGAF00001704319 fq.gz 673.0 MB
EGAF00001704320 fq.gz 783.8 MB
EGAF00001704321 fq.gz 780.4 MB
EGAF00001704322 fq.gz 769.4 MB
EGAF00001704323 fq.gz 642.6 MB
EGAF00001704324 fq.gz 672.1 MB
EGAF00001704325 fq.gz 681.6 MB
EGAF00001704326 fq.gz 549.3 MB
EGAF00001704327 fq.gz 570.1 MB
EGAF00001704328 fq.gz 546.6 MB
EGAF00001704329 fq.gz 602.1 MB
EGAF00001704330 fq.gz 686.8 MB
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EGAF00001704332 fq.gz 674.9 MB
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EGAF00001704334 fq.gz 832.2 MB
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EGAF00001704340 fq.gz 395.9 MB
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EGAF00001704342 fq.gz 398.6 MB
EGAF00001704343 fq.gz 402.4 MB
EGAF00001704344 fq.gz 402.9 MB
EGAF00001704345 fq.gz 373.4 MB
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EGAF00001704347 fq.gz 366.5 MB
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EGAF00001704349 fq.gz 373.2 MB
EGAF00001704350 fq.gz 368.0 MB
EGAF00001704351 fq.gz 370.5 MB
EGAF00001704352 fq.gz 370.4 MB
EGAF00001704353 fq.gz 344.4 MB
EGAF00001704354 fq.gz 267.4 MB
EGAF00001704355 fq.gz 266.8 MB
EGAF00001704356 fq.gz 266.9 MB
EGAF00001704357 fq.gz 405.9 MB
EGAF00001704358 fq.gz 402.1 MB
EGAF00001704359 fq.gz 382.0 MB
EGAF00001704360 fq.gz 377.9 MB
EGAF00001704361 fq.gz 378.9 MB
EGAF00001704362 fq.gz 379.4 MB
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EGAF00001704386 fq.gz 315.5 MB
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EGAF00001704421 fq.gz 902.9 MB
EGAF00001704422 fq.gz 824.6 MB
EGAF00001704423 fq.gz 1.1 GB
EGAF00001704424 fq.gz 1.1 GB
EGAF00001704425 fq.gz 1.1 GB
EGAF00001704426 fq.gz 1.4 GB
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EGAF00001704428 fq.gz 587.4 MB
EGAF00001704429 fq.gz 1.4 GB
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EGAF00001704436 fq.gz 800.4 MB
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EGAF00001704442 fq.gz 675.0 MB
EGAF00001704443 fq.gz 747.1 MB
EGAF00001704444 fq.gz 874.2 MB
EGAF00001704445 fq.gz 871.1 MB
EGAF00001704446 fq.gz 858.4 MB
EGAF00001704447 fq.gz 637.4 MB
EGAF00001704448 fq.gz 671.1 MB
EGAF00001704449 fq.gz 695.9 MB
EGAF00001704450 fq.gz 542.6 MB
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EGAF00001704465 fq.gz 249.2 MB
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EGAF00001704467 fq.gz 316.5 MB
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EGAF00001704472 fq.gz 319.5 MB
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EGAF00001704476 fq.gz 294.8 MB
EGAF00001704477 fq.gz 295.7 MB
EGAF00001704478 fq.gz 291.9 MB
EGAF00001704479 fq.gz 293.5 MB
EGAF00001704480 fq.gz 400.0 MB
EGAF00001704481 fq.gz 395.9 MB
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2186 Files (1.2 TB)