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FASTQ files of total RNA-Seq data from the POPS PET (pre-eclamptic) samples

All the samples were obtained from the Pregnancy Outcome Prediction–a prospective cohort study of nulliparous women attending the Rosie Hospital, Cambridge (UK) for their dating ultrasound scan between January 14, 2008, and July 31, 2012. Ethical approval for the study was given by the Cambridgeshire 2 Research Ethics Committee (reference number 07/H0308/163) and all participants provided written informed consent. Cases of preeclampsia (PET) were defined on the basis of the 2013 ACOG criteria and cases of small for gestational age (SGA)infants were confined to severe SGA, i.e. a customized birth weight <5th percentile. Chorionic villi from the corresponding placentas (free from decidua, visible infarction, calcification, hematoma, or damage) were collected and processed within 30 minutes of separation from the uterus. After repeated washes in chilled phosphate buffered saline, the samples were placed in RNA later (Applied Biosystems) and stored at -80°C. Total placental RNA was extracted using mirVana Isolation Kit (Ambion). For each placenta, approximately 5 mg of tissue were homogenized in the Lysis/Binding solution for 20 sec at 6 m/s using a bead beater (FastPrep24) and Lysing Matrix D Tubes (MP Biomedicals). The samples were then spun at 13,000 rpm for 5 min at 4°C and the supernatants recovered. Afterwards, the manufacturer's instructions were followed. Immediately after the RNA extraction, placental RNA samples were DNase-treated using DNA-free DNA Removal Kit (Ambion), aliquoted, and stored in -80°C. Quantity and quality of the RNA samples were assessed using the Agilent 2100 Bioanalyzer, the Agilent RNA 6000 Nano Kit (Agilent Technologies), and Qubit fluorometer. Libraries were prepared starting with 300-500 ng of good quality total RNA (RIN ≥7.5) using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat (Illumina), according to the manufacturer's instructions. The kit contains 96 uniquely indexed adapter combinations in order to allow pooling of multiple samples prior to sequencing. After determining their size (with the Agilent 2100 Bioanalyzer and the Agilent High Sensitivity DNA Kit by Agilent Technologies) and concentration (by qPCR with the KAPA Illumina ABI Prism Library Quantification Kit, Kapa Biosystems), libraries have been pooled and sequenced (single-end, 125 bp) using a Single End V4 Cluster Kit and an Illumina HiSeq2500 or HiSeq4000 instrument.

Request Access

DUO:0000006
version: 2019-01-07

health or medical or biomedical research

This data use permission indicates that use is allowed for health/medical/biomedical purposes; does not include the study of population origins or ancestry.

DUO:0000026
version: 2019-01-07

user specific restriction

This data use modifier indicates that use is limited to use by approved users.

POPS data access policy

You are not authroised to access POPS data unless you have a written permission from the data access committee which you can contact below: D. Stephen Charnock-Jones. Professor of Reproductive Biology. Department of Obstetrics and Gynaecology. University of Cambridge. The Rosie Hospital, Cambridge. CB2 0SW UK email dscj1@cam.ac.uk Tel +44 1223 336875 Fax +44 1223 215327

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001002205 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
EGAF00001707801 fq.gz 624.0 MB Report
EGAF00001707802 fq.gz 844.2 MB Report
EGAF00001707803 fq.gz 848.0 MB Report
EGAF00001707804 fq.gz 814.7 MB Report
EGAF00001707805 fq.gz 778.4 MB Report
EGAF00001707806 fq.gz 891.9 MB Report
EGAF00001707807 fq.gz 815.6 MB Report
EGAF00001707808 fq.gz 827.0 MB Report
EGAF00001707809 fq.gz 973.2 MB Report
EGAF00001707810 fq.gz 670.4 MB Report
EGAF00001707811 fq.gz 1.1 GB Report
EGAF00001707812 fq.gz 1.1 GB Report
EGAF00001707813 fq.gz 1.1 GB Report
EGAF00001707814 fq.gz 1.1 GB Report
EGAF00001707815 fq.gz 1.1 GB Report
EGAF00001707816 fq.gz 821.1 MB Report
EGAF00001707817 fq.gz 966.9 MB Report
EGAF00001707818 fq.gz 877.4 MB Report
EGAF00001707819 fq.gz 858.7 MB Report
EGAF00001707820 fq.gz 958.0 MB Report
EGAF00001707821 fq.gz 958.8 MB Report
EGAF00001707822 fq.gz 956.8 MB Report
EGAF00001707823 fq.gz 947.2 MB Report
EGAF00001707824 fq.gz 972.2 MB Report
EGAF00001707825 fq.gz 1.1 GB Report
EGAF00001707826 fq.gz 680.6 MB Report
EGAF00001707827 fq.gz 624.7 MB Report
EGAF00001707828 fq.gz 693.8 MB Report
EGAF00001707829 fq.gz 700.9 MB Report
EGAF00001707830 fq.gz 565.5 MB Report
EGAF00001707831 fq.gz 584.2 MB Report
EGAF00001707832 fq.gz 564.2 MB Report
EGAF00001707833 fq.gz 619.5 MB Report
EGAF00001707834 fq.gz 709.4 MB Report
EGAF00001707835 fq.gz 707.6 MB Report
EGAF00001707836 fq.gz 697.1 MB Report
EGAF00001707837 fq.gz 762.5 MB Report
EGAF00001707838 fq.gz 779.6 MB Report
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EGAF00001707904 fq.gz 985.2 MB Report
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EGAF00001707906 fq.gz 931.2 MB Report
EGAF00001707907 fq.gz 896.1 MB Report
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EGAF00001707909 fq.gz 963.2 MB Report
EGAF00001707910 fq.gz 888.5 MB Report
EGAF00001707911 fq.gz 907.4 MB Report
EGAF00001707912 fq.gz 1.1 GB Report
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EGAF00001707915 fq.gz 938.1 MB Report
EGAF00001707916 fq.gz 890.2 MB Report
EGAF00001707917 fq.gz 1.0 GB Report
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EGAF00001707922 fq.gz 784.5 MB Report
EGAF00001707923 fq.gz 1.2 GB Report
EGAF00001707924 fq.gz 680.7 MB Report
EGAF00001707925 fq.gz 681.3 MB Report
EGAF00001707926 fq.gz 677.0 MB Report
EGAF00001707927 fq.gz 668.4 MB Report
EGAF00001707928 fq.gz 689.0 MB Report
EGAF00001707929 fq.gz 481.6 MB Report
EGAF00001707930 fq.gz 785.6 MB Report
EGAF00001707931 fq.gz 723.0 MB Report
EGAF00001707932 fq.gz 519.1 MB Report
EGAF00001707933 fq.gz 780.7 MB Report
EGAF00001707934 fq.gz 782.1 MB Report
EGAF00001707935 fq.gz 777.6 MB Report
EGAF00001707936 fq.gz 767.7 MB Report
EGAF00001707937 fq.gz 797.3 MB Report
EGAF00001707938 fq.gz 798.4 MB Report
EGAF00001707939 fq.gz 880.1 MB Report
EGAF00001707940 fq.gz 800.2 MB Report
EGAF00001707941 fq.gz 904.8 MB Report
EGAF00001707942 fq.gz 612.5 MB Report
EGAF00001707943 fq.gz 653.9 MB Report
EGAF00001707944 fq.gz 677.5 MB Report
EGAF00001707945 fq.gz 527.0 MB Report
EGAF00001707946 fq.gz 545.9 MB Report
EGAF00001707947 fq.gz 525.1 MB Report
EGAF00001707948 fq.gz 582.5 MB Report
EGAF00001707949 fq.gz 688.7 MB Report
EGAF00001707950 fq.gz 684.5 MB Report
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EGAF00001707954 fq.gz 785.6 MB Report
EGAF00001707955 fq.gz 628.3 MB Report
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EGAF00001707960 fq.gz 796.6 MB Report
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EGAF00001708019 fq.gz 707.9 MB Report
EGAF00001708020 fq.gz 763.4 MB Report
EGAF00001708021 fq.gz 757.1 MB Report
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EGAF00001708023 fq.gz 762.4 MB Report
EGAF00001708024 fq.gz 789.4 MB Report
EGAF00001708025 fq.gz 792.3 MB Report
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EGAF00001708027 fq.gz 707.6 MB Report
EGAF00001708028 fq.gz 755.0 MB Report
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EGAF00001708030 fq.gz 769.6 MB Report
EGAF00001708031 fq.gz 700.6 MB Report
EGAF00001708032 fq.gz 858.9 MB Report
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EGAF00001708047 fq.gz 785.6 MB Report
EGAF00001708048 fq.gz 781.0 MB Report
EGAF00001708049 fq.gz 771.2 MB Report
EGAF00001708050 fq.gz 791.5 MB Report
EGAF00001708051 fq.gz 837.4 MB Report
EGAF00001708052 fq.gz 883.8 MB Report
EGAF00001708053 fq.gz 797.6 MB Report
EGAF00001708054 fq.gz 1.0 GB Report
EGAF00001708055 fq.gz 904.0 MB Report
EGAF00001708056 fq.gz 788.3 MB Report
EGAF00001708057 fq.gz 795.0 MB Report
EGAF00001708058 fq.gz 846.0 MB Report
EGAF00001708059 fq.gz 880.9 MB Report
EGAF00001708060 fq.gz 691.1 MB Report
EGAF00001708061 fq.gz 713.9 MB Report
EGAF00001708062 fq.gz 682.2 MB Report
EGAF00001708063 fq.gz 758.4 MB Report
EGAF00001708064 fq.gz 904.3 MB Report
EGAF00001708065 fq.gz 897.2 MB Report
EGAF00001708066 fq.gz 886.4 MB Report
EGAF00001708067 fq.gz 803.5 MB Report
EGAF00001708068 fq.gz 829.8 MB Report
EGAF00001708069 fq.gz 855.2 MB Report
EGAF00001708070 fq.gz 679.2 MB Report
EGAF00001708071 fq.gz 703.7 MB Report
EGAF00001708072 fq.gz 675.5 MB Report
EGAF00001708073 fq.gz 747.6 MB Report
EGAF00001708074 fq.gz 877.1 MB Report
EGAF00001708132 fq.gz 828.7 MB Report
EGAF00001708133 fq.gz 832.1 MB Report
EGAF00001708134 fq.gz 683.6 MB Report
EGAF00001708135 fq.gz 1.4 GB Report
EGAF00001708136 fq.gz 1.4 GB Report
EGAF00001708137 fq.gz 1.4 GB Report
EGAF00001708138 fq.gz 1.3 GB Report
EGAF00001708139 fq.gz 1.4 GB Report
EGAF00001708140 fq.gz 1.4 GB Report
EGAF00001708141 fq.gz 1.4 GB Report
EGAF00001708142 fq.gz 799.4 MB Report
EGAF00001708143 fq.gz 770.6 MB Report
EGAF00001708144 fq.gz 772.9 MB Report
EGAF00001708145 fq.gz 748.7 MB Report
EGAF00001708146 fq.gz 712.4 MB Report
EGAF00001708147 fq.gz 762.6 MB Report
EGAF00001708148 fq.gz 753.3 MB Report
EGAF00001708149 fq.gz 752.5 MB Report
EGAF00001708150 fq.gz 779.3 MB Report
EGAF00001708151 fq.gz 904.5 MB Report
EGAF00001708152 fq.gz 903.4 MB Report
EGAF00001708153 fq.gz 887.2 MB Report
EGAF00001708154 fq.gz 913.6 MB Report
EGAF00001708155 fq.gz 461.9 MB Report
EGAF00001708156 fq.gz 586.9 MB Report
EGAF00001708157 fq.gz 529.3 MB Report
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EGAF00001708159 fq.gz 602.4 MB Report
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EGAF00001708161 fq.gz 602.8 MB Report
EGAF00001708162 fq.gz 592.0 MB Report
EGAF00001708163 fq.gz 612.5 MB Report
EGAF00001708164 fq.gz 351.5 MB Report
EGAF00001708165 fq.gz 891.3 MB Report
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EGAF00001708167 fq.gz 421.5 MB Report
EGAF00001708168 fq.gz 913.4 MB Report
EGAF00001708169 fq.gz 912.1 MB Report
EGAF00001708170 fq.gz 871.0 MB Report
EGAF00001708171 fq.gz 860.9 MB Report
EGAF00001708172 fq.gz 745.3 MB Report
EGAF00001708173 fq.gz 860.3 MB Report
EGAF00001708174 fq.gz 911.1 MB Report
EGAF00001708175 fq.gz 695.7 MB Report
EGAF00001708176 fq.gz 719.9 MB Report
EGAF00001708177 fq.gz 691.0 MB Report
EGAF00001708178 fq.gz 775.8 MB Report
EGAF00001708179 fq.gz 950.1 MB Report
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EGAF00001708181 fq.gz 931.6 MB Report
EGAF00001708182 fq.gz 708.6 MB Report
EGAF00001708183 fq.gz 781.8 MB Report
EGAF00001708184 fq.gz 819.8 MB Report
EGAF00001708185 fq.gz 633.7 MB Report
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EGAF00001708187 fq.gz 629.7 MB Report
EGAF00001708188 fq.gz 701.1 MB Report
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EGAF00001708260 fq.gz 796.6 MB Report
EGAF00001708261 fq.gz 769.2 MB Report
EGAF00001708262 fq.gz 725.1 MB Report
EGAF00001708263 fq.gz 771.2 MB Report
EGAF00001708264 fq.gz 767.7 MB Report
EGAF00001708265 fq.gz 780.0 MB Report
EGAF00001708266 fq.gz 987.4 MB Report
EGAF00001708267 fq.gz 945.1 MB Report
EGAF00001708268 fq.gz 948.6 MB Report
EGAF00001708269 fq.gz 912.9 MB Report
EGAF00001708270 fq.gz 864.7 MB Report
EGAF00001708271 fq.gz 941.6 MB Report
EGAF00001708272 fq.gz 935.3 MB Report
EGAF00001708273 fq.gz 944.0 MB Report
EGAF00001708274 fq.gz 1.0 GB Report
EGAF00001708275 fq.gz 900.8 MB Report
EGAF00001708276 fq.gz 904.8 MB Report
EGAF00001708277 fq.gz 876.0 MB Report
EGAF00001708278 fq.gz 913.6 MB Report
EGAF00001708279 fq.gz 897.6 MB Report
EGAF00001708280 fq.gz 911.1 MB Report
EGAF00001708281 fq.gz 813.5 MB Report
EGAF00001708282 fq.gz 547.5 MB Report
EGAF00001708283 fq.gz 486.0 MB Report
EGAF00001708284 fq.gz 866.5 MB Report
EGAF00001708285 fq.gz 555.7 MB Report
EGAF00001708286 fq.gz 551.2 MB Report
EGAF00001708287 fq.gz 555.9 MB Report
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EGAF00001708289 fq.gz 555.1 MB Report
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EGAF00001708291 fq.gz 519.5 MB Report
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EGAF00001708301 fq.gz 814.7 MB Report
EGAF00001708302 fq.gz 866.1 MB Report
EGAF00001708303 fq.gz 659.1 MB Report
EGAF00001708304 fq.gz 681.2 MB Report
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EGAF00001708311 fq.gz 753.3 MB Report
EGAF00001708312 fq.gz 791.4 MB Report
EGAF00001708313 fq.gz 613.3 MB Report
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EGAF00001708315 fq.gz 608.0 MB Report
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EGAF00001708407 fq.gz 851.0 MB Report
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EGAF00001708413 fq.gz 549.0 MB Report
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EGAF00001709809 fq.gz 659.2 MB Report
EGAF00001709810 fq.gz 649.5 MB Report
EGAF00001709811 fq.gz 689.5 MB Report
831 Files (658.0 GB)