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FASTQ files of total RNA-Seq data from the POPS PET (pre-eclamptic) samples

All the samples were obtained from the Pregnancy Outcome Prediction–a prospective cohort study of nulliparous women attending the Rosie Hospital, Cambridge (UK) for their dating ultrasound scan between January 14, 2008, and July 31, 2012. Ethical approval for the study was given by the Cambridgeshire 2 Research Ethics Committee (reference number 07/H0308/163) and all participants provided written informed consent. Cases of preeclampsia (PET) were defined on the basis of the 2013 ACOG criteria and cases of small for gestational age (SGA)infants were confined to severe SGA, i.e. a customized birth weight <5th percentile. Chorionic villi from the corresponding placentas (free from decidua, visible infarction, calcification, hematoma, or damage) were collected and processed within 30 minutes of separation from the uterus. After repeated washes in chilled phosphate buffered saline, the samples were placed in RNA later (Applied Biosystems) and stored at -80°C. Total placental RNA was extracted using mirVana Isolation Kit (Ambion). For each placenta, approximately 5 mg of tissue were homogenized in the Lysis/Binding solution for 20 sec at 6 m/s using a bead beater (FastPrep24) and Lysing Matrix D Tubes (MP Biomedicals). The samples were then spun at 13,000 rpm for 5 min at 4°C and the supernatants recovered. Afterwards, the manufacturer's instructions were followed. Immediately after the RNA extraction, placental RNA samples were DNase-treated using DNA-free DNA Removal Kit (Ambion), aliquoted, and stored in -80°C. Quantity and quality of the RNA samples were assessed using the Agilent 2100 Bioanalyzer, the Agilent RNA 6000 Nano Kit (Agilent Technologies), and Qubit fluorometer. Libraries were prepared starting with 300-500 ng of good quality total RNA (RIN ≥7.5) using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat (Illumina), according to the manufacturer's instructions. The kit contains 96 uniquely indexed adapter combinations in order to allow pooling of multiple samples prior to sequencing. After determining their size (with the Agilent 2100 Bioanalyzer and the Agilent High Sensitivity DNA Kit by Agilent Technologies) and concentration (by qPCR with the KAPA Illumina ABI Prism Library Quantification Kit, Kapa Biosystems), libraries have been pooled and sequenced (single-end, 125 bp) using a Single End V4 Cluster Kit and an Illumina HiSeq2500 or HiSeq4000 instrument.

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POPS data access policy

You are not authroised to access POPS data unless you have a written permission from the data access committee which you can contact below: D. Stephen Charnock-Jones. Professor of Reproductive Biology. Department of Obstetrics and Gynaecology. University of Cambridge. The Rosie Hospital, Cambridge. CB2 0SW UK email dscj1@cam.ac.uk Tel +44 1223 336875 Fax +44 1223 215327

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001002205 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00001707801 fq.gz 624.0 MB
EGAF00001707802 fq.gz 844.2 MB
EGAF00001707803 fq.gz 848.0 MB
EGAF00001707804 fq.gz 814.7 MB
EGAF00001707805 fq.gz 778.4 MB
EGAF00001707806 fq.gz 891.9 MB
EGAF00001707807 fq.gz 815.6 MB
EGAF00001707808 fq.gz 827.0 MB
EGAF00001707809 fq.gz 973.2 MB
EGAF00001707810 fq.gz 670.4 MB
EGAF00001707811 fq.gz 1.1 GB
EGAF00001707812 fq.gz 1.1 GB
EGAF00001707813 fq.gz 1.1 GB
EGAF00001707814 fq.gz 1.1 GB
EGAF00001707815 fq.gz 1.1 GB
EGAF00001707816 fq.gz 821.1 MB
EGAF00001707817 fq.gz 966.9 MB
EGAF00001707818 fq.gz 877.4 MB
EGAF00001707819 fq.gz 858.7 MB
EGAF00001707820 fq.gz 958.0 MB
EGAF00001707821 fq.gz 958.8 MB
EGAF00001707822 fq.gz 956.8 MB
EGAF00001707823 fq.gz 947.2 MB
EGAF00001707824 fq.gz 972.2 MB
EGAF00001707825 fq.gz 1.1 GB
EGAF00001707826 fq.gz 680.6 MB
EGAF00001707827 fq.gz 624.7 MB
EGAF00001707828 fq.gz 693.8 MB
EGAF00001707829 fq.gz 700.9 MB
EGAF00001707830 fq.gz 565.5 MB
EGAF00001707831 fq.gz 584.2 MB
EGAF00001707832 fq.gz 564.2 MB
EGAF00001707833 fq.gz 619.5 MB
EGAF00001707834 fq.gz 709.4 MB
EGAF00001707835 fq.gz 707.6 MB
EGAF00001707836 fq.gz 697.1 MB
EGAF00001707837 fq.gz 762.5 MB
EGAF00001707838 fq.gz 779.6 MB
EGAF00001707839 fq.gz 791.2 MB
EGAF00001707840 fq.gz 640.8 MB
EGAF00001707841 fq.gz 663.3 MB
EGAF00001707842 fq.gz 639.1 MB
EGAF00001707843 fq.gz 703.0 MB
EGAF00001707844 fq.gz 806.4 MB
EGAF00001707845 fq.gz 804.9 MB
EGAF00001707846 fq.gz 793.5 MB
EGAF00001707904 fq.gz 985.2 MB
EGAF00001707905 fq.gz 927.2 MB
EGAF00001707906 fq.gz 931.2 MB
EGAF00001707907 fq.gz 896.1 MB
EGAF00001707908 fq.gz 849.4 MB
EGAF00001707909 fq.gz 963.2 MB
EGAF00001707910 fq.gz 888.5 MB
EGAF00001707911 fq.gz 907.4 MB
EGAF00001707912 fq.gz 1.1 GB
EGAF00001707913 fq.gz 969.3 MB
EGAF00001707914 fq.gz 975.7 MB
EGAF00001707915 fq.gz 938.1 MB
EGAF00001707916 fq.gz 890.2 MB
EGAF00001707917 fq.gz 1.0 GB
EGAF00001707918 fq.gz 930.2 MB
EGAF00001707919 fq.gz 942.8 MB
EGAF00001707920 fq.gz 782.5 MB
EGAF00001707921 fq.gz 781.8 MB
EGAF00001707922 fq.gz 784.5 MB
EGAF00001707923 fq.gz 1.2 GB
EGAF00001707924 fq.gz 680.7 MB
EGAF00001707925 fq.gz 681.3 MB
EGAF00001707926 fq.gz 677.0 MB
EGAF00001707927 fq.gz 668.4 MB
EGAF00001707928 fq.gz 689.0 MB
EGAF00001707929 fq.gz 481.6 MB
EGAF00001707930 fq.gz 785.6 MB
EGAF00001707931 fq.gz 723.0 MB
EGAF00001707932 fq.gz 519.1 MB
EGAF00001707933 fq.gz 780.7 MB
EGAF00001707934 fq.gz 782.1 MB
EGAF00001707935 fq.gz 777.6 MB
EGAF00001707936 fq.gz 767.7 MB
EGAF00001707937 fq.gz 797.3 MB
EGAF00001707938 fq.gz 798.4 MB
EGAF00001707939 fq.gz 880.1 MB
EGAF00001707940 fq.gz 800.2 MB
EGAF00001707941 fq.gz 904.8 MB
EGAF00001707942 fq.gz 612.5 MB
EGAF00001707943 fq.gz 653.9 MB
EGAF00001707944 fq.gz 677.5 MB
EGAF00001707945 fq.gz 527.0 MB
EGAF00001707946 fq.gz 545.9 MB
EGAF00001707947 fq.gz 525.1 MB
EGAF00001707948 fq.gz 582.5 MB
EGAF00001707949 fq.gz 688.7 MB
EGAF00001707950 fq.gz 684.5 MB
EGAF00001707951 fq.gz 677.1 MB
EGAF00001707952 fq.gz 746.4 MB
EGAF00001707953 fq.gz 765.6 MB
EGAF00001707954 fq.gz 785.6 MB
EGAF00001707955 fq.gz 628.3 MB
EGAF00001707956 fq.gz 647.4 MB
EGAF00001707957 fq.gz 623.4 MB
EGAF00001707958 fq.gz 688.2 MB
EGAF00001707959 fq.gz 801.5 MB
EGAF00001707960 fq.gz 796.6 MB
EGAF00001708018 fq.gz 759.5 MB
EGAF00001708019 fq.gz 707.9 MB
EGAF00001708020 fq.gz 763.4 MB
EGAF00001708021 fq.gz 757.1 MB
EGAF00001708022 fq.gz 761.5 MB
EGAF00001708023 fq.gz 762.4 MB
EGAF00001708024 fq.gz 789.4 MB
EGAF00001708025 fq.gz 792.3 MB
EGAF00001708026 fq.gz 764.3 MB
EGAF00001708027 fq.gz 707.6 MB
EGAF00001708028 fq.gz 755.0 MB
EGAF00001708029 fq.gz 758.8 MB
EGAF00001708030 fq.gz 769.6 MB
EGAF00001708031 fq.gz 700.6 MB
EGAF00001708032 fq.gz 858.9 MB
EGAF00001708033 fq.gz 863.0 MB
EGAF00001708034 fq.gz 834.7 MB
EGAF00001708035 fq.gz 789.1 MB
EGAF00001708036 fq.gz 847.5 MB
EGAF00001708037 fq.gz 885.0 MB
EGAF00001708038 fq.gz 888.7 MB
EGAF00001708039 fq.gz 879.7 MB
EGAF00001708040 fq.gz 868.4 MB
EGAF00001708041 fq.gz 886.6 MB
EGAF00001708042 fq.gz 658.5 MB
EGAF00001708043 fq.gz 787.8 MB
EGAF00001708044 fq.gz 727.4 MB
EGAF00001708045 fq.gz 692.4 MB
EGAF00001708046 fq.gz 784.7 MB
EGAF00001708047 fq.gz 785.6 MB
EGAF00001708048 fq.gz 781.0 MB
EGAF00001708049 fq.gz 771.2 MB
EGAF00001708050 fq.gz 791.5 MB
EGAF00001708051 fq.gz 837.4 MB
EGAF00001708052 fq.gz 883.8 MB
EGAF00001708053 fq.gz 797.6 MB
EGAF00001708054 fq.gz 1.0 GB
EGAF00001708055 fq.gz 904.0 MB
EGAF00001708056 fq.gz 788.3 MB
EGAF00001708057 fq.gz 795.0 MB
EGAF00001708058 fq.gz 846.0 MB
EGAF00001708059 fq.gz 880.9 MB
EGAF00001708060 fq.gz 691.1 MB
EGAF00001708061 fq.gz 713.9 MB
EGAF00001708062 fq.gz 682.2 MB
EGAF00001708063 fq.gz 758.4 MB
EGAF00001708064 fq.gz 904.3 MB
EGAF00001708065 fq.gz 897.2 MB
EGAF00001708066 fq.gz 886.4 MB
EGAF00001708067 fq.gz 803.5 MB
EGAF00001708068 fq.gz 829.8 MB
EGAF00001708069 fq.gz 855.2 MB
EGAF00001708070 fq.gz 679.2 MB
EGAF00001708071 fq.gz 703.7 MB
EGAF00001708072 fq.gz 675.5 MB
EGAF00001708073 fq.gz 747.6 MB
EGAF00001708074 fq.gz 877.1 MB
EGAF00001708132 fq.gz 828.7 MB
EGAF00001708133 fq.gz 832.1 MB
EGAF00001708134 fq.gz 683.6 MB
EGAF00001708135 fq.gz 1.4 GB
EGAF00001708136 fq.gz 1.4 GB
EGAF00001708137 fq.gz 1.4 GB
EGAF00001708138 fq.gz 1.3 GB
EGAF00001708139 fq.gz 1.4 GB
EGAF00001708140 fq.gz 1.4 GB
EGAF00001708141 fq.gz 1.4 GB
EGAF00001708142 fq.gz 799.4 MB
EGAF00001708143 fq.gz 770.6 MB
EGAF00001708144 fq.gz 772.9 MB
EGAF00001708145 fq.gz 748.7 MB
EGAF00001708146 fq.gz 712.4 MB
EGAF00001708147 fq.gz 762.6 MB
EGAF00001708148 fq.gz 753.3 MB
EGAF00001708149 fq.gz 752.5 MB
EGAF00001708150 fq.gz 779.3 MB
EGAF00001708151 fq.gz 904.5 MB
EGAF00001708152 fq.gz 903.4 MB
EGAF00001708153 fq.gz 887.2 MB
EGAF00001708154 fq.gz 913.6 MB
EGAF00001708155 fq.gz 461.9 MB
EGAF00001708156 fq.gz 586.9 MB
EGAF00001708157 fq.gz 529.3 MB
EGAF00001708158 fq.gz 482.8 MB
EGAF00001708159 fq.gz 602.4 MB
EGAF00001708160 fq.gz 601.8 MB
EGAF00001708161 fq.gz 602.8 MB
EGAF00001708162 fq.gz 592.0 MB
EGAF00001708163 fq.gz 612.5 MB
EGAF00001708164 fq.gz 351.5 MB
EGAF00001708165 fq.gz 891.3 MB
EGAF00001708166 fq.gz 779.9 MB
EGAF00001708167 fq.gz 421.5 MB
EGAF00001708168 fq.gz 913.4 MB
EGAF00001708169 fq.gz 912.1 MB
EGAF00001708170 fq.gz 871.0 MB
EGAF00001708171 fq.gz 860.9 MB
EGAF00001708172 fq.gz 745.3 MB
EGAF00001708173 fq.gz 860.3 MB
EGAF00001708174 fq.gz 911.1 MB
EGAF00001708175 fq.gz 695.7 MB
EGAF00001708176 fq.gz 719.9 MB
EGAF00001708177 fq.gz 691.0 MB
EGAF00001708178 fq.gz 775.8 MB
EGAF00001708179 fq.gz 950.1 MB
EGAF00001708180 fq.gz 939.3 MB
EGAF00001708181 fq.gz 931.6 MB
EGAF00001708182 fq.gz 708.6 MB
EGAF00001708183 fq.gz 781.8 MB
EGAF00001708184 fq.gz 819.8 MB
EGAF00001708185 fq.gz 633.7 MB
EGAF00001708186 fq.gz 653.4 MB
EGAF00001708187 fq.gz 629.7 MB
EGAF00001708188 fq.gz 701.1 MB
EGAF00001708259 fq.gz 794.2 MB
EGAF00001708260 fq.gz 796.6 MB
EGAF00001708261 fq.gz 769.2 MB
EGAF00001708262 fq.gz 725.1 MB
EGAF00001708263 fq.gz 771.2 MB
EGAF00001708264 fq.gz 767.7 MB
EGAF00001708265 fq.gz 780.0 MB
EGAF00001708266 fq.gz 987.4 MB
EGAF00001708267 fq.gz 945.1 MB
EGAF00001708268 fq.gz 948.6 MB
EGAF00001708269 fq.gz 912.9 MB
EGAF00001708270 fq.gz 864.7 MB
EGAF00001708271 fq.gz 941.6 MB
EGAF00001708272 fq.gz 935.3 MB
EGAF00001708273 fq.gz 944.0 MB
EGAF00001708274 fq.gz 1.0 GB
EGAF00001708275 fq.gz 900.8 MB
EGAF00001708276 fq.gz 904.8 MB
EGAF00001708277 fq.gz 876.0 MB
EGAF00001708278 fq.gz 913.6 MB
EGAF00001708279 fq.gz 897.6 MB
EGAF00001708280 fq.gz 911.1 MB
EGAF00001708281 fq.gz 813.5 MB
EGAF00001708282 fq.gz 547.5 MB
EGAF00001708283 fq.gz 486.0 MB
EGAF00001708284 fq.gz 866.5 MB
EGAF00001708285 fq.gz 555.7 MB
EGAF00001708286 fq.gz 551.2 MB
EGAF00001708287 fq.gz 555.9 MB
EGAF00001708288 fq.gz 545.8 MB
EGAF00001708289 fq.gz 555.1 MB
EGAF00001708290 fq.gz 536.3 MB
EGAF00001708291 fq.gz 519.5 MB
EGAF00001708292 fq.gz 479.2 MB
EGAF00001708293 fq.gz 466.2 MB
EGAF00001708294 fq.gz 472.4 MB
EGAF00001708295 fq.gz 458.6 MB
EGAF00001708296 fq.gz 541.0 MB
EGAF00001708297 fq.gz 846.8 MB
EGAF00001708298 fq.gz 838.7 MB
EGAF00001708299 fq.gz 830.9 MB
EGAF00001708300 fq.gz 715.2 MB
EGAF00001708301 fq.gz 814.7 MB
EGAF00001708302 fq.gz 866.1 MB
EGAF00001708303 fq.gz 659.1 MB
EGAF00001708304 fq.gz 681.2 MB
EGAF00001708305 fq.gz 650.2 MB
EGAF00001708306 fq.gz 730.0 MB
EGAF00001708307 fq.gz 904.3 MB
EGAF00001708308 fq.gz 892.7 MB
EGAF00001708309 fq.gz 883.3 MB
EGAF00001708310 fq.gz 685.8 MB
EGAF00001708311 fq.gz 753.3 MB
EGAF00001708312 fq.gz 791.4 MB
EGAF00001708313 fq.gz 613.3 MB
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EGAF00001708315 fq.gz 608.0 MB
EGAF00001708392 fq.gz 830.3 MB
EGAF00001708393 fq.gz 893.2 MB
EGAF00001708394 fq.gz 896.2 MB
EGAF00001708395 fq.gz 897.5 MB
EGAF00001708396 fq.gz 1.1 GB
EGAF00001708397 fq.gz 994.5 MB
EGAF00001708398 fq.gz 998.3 MB
EGAF00001708399 fq.gz 965.3 MB
EGAF00001708400 fq.gz 909.7 MB
EGAF00001708401 fq.gz 973.0 MB
EGAF00001708402 fq.gz 977.9 MB
EGAF00001708403 fq.gz 993.9 MB
EGAF00001708404 fq.gz 952.7 MB
EGAF00001708405 fq.gz 875.1 MB
EGAF00001708406 fq.gz 879.7 MB
EGAF00001708407 fq.gz 851.0 MB
EGAF00001708408 fq.gz 802.3 MB
EGAF00001708409 fq.gz 869.6 MB
EGAF00001708410 fq.gz 863.1 MB
EGAF00001708411 fq.gz 542.3 MB
EGAF00001708412 fq.gz 548.6 MB
EGAF00001708413 fq.gz 549.0 MB
EGAF00001708414 fq.gz 553.9 MB
EGAF00001708415 fq.gz 476.3 MB
EGAF00001708416 fq.gz 738.0 MB
EGAF00001708417 fq.gz 691.1 MB
EGAF00001708418 fq.gz 671.8 MB
EGAF00001708419 fq.gz 681.6 MB
EGAF00001708420 fq.gz 662.2 MB
EGAF00001708421 fq.gz 772.0 MB
EGAF00001708422 fq.gz 769.6 MB
EGAF00001708423 fq.gz 777.9 MB
EGAF00001708424 fq.gz 781.2 MB
EGAF00001708425 fq.gz 787.5 MB
EGAF00001708426 fq.gz 360.4 MB
EGAF00001708427 fq.gz 713.6 MB
EGAF00001708428 fq.gz 657.2 MB
EGAF00001708429 fq.gz 638.9 MB
EGAF00001708430 fq.gz 679.0 MB
EGAF00001708431 fq.gz 822.4 MB
EGAF00001708432 fq.gz 814.3 MB
EGAF00001708433 fq.gz 805.9 MB
EGAF00001708434 fq.gz 790.1 MB
EGAF00001708435 fq.gz 797.1 MB
EGAF00001708436 fq.gz 776.8 MB
EGAF00001708437 fq.gz 799.3 MB
EGAF00001708438 fq.gz 782.1 MB
EGAF00001708439 fq.gz 800.4 MB
EGAF00001708440 fq.gz 656.4 MB
EGAF00001708441 fq.gz 741.1 MB
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EGAF00001708446 fq.gz 735.4 MB
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EGAF00001708448 fq.gz 734.4 MB
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EGAF00001708528 fq.gz 765.5 MB
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EGAF00001708530 fq.gz 833.7 MB
EGAF00001708531 fq.gz 686.9 MB
EGAF00001708532 fq.gz 713.0 MB
EGAF00001708533 fq.gz 717.1 MB
EGAF00001708534 fq.gz 713.7 MB
EGAF00001708535 fq.gz 563.6 MB
EGAF00001708536 fq.gz 649.4 MB
EGAF00001708537 fq.gz 801.2 MB
EGAF00001708538 fq.gz 753.1 MB
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EGAF00001708544 fq.gz 648.8 MB
EGAF00001708545 fq.gz 629.0 MB
EGAF00001708546 fq.gz 728.9 MB
EGAF00001708547 fq.gz 746.3 MB
EGAF00001708548 fq.gz 754.1 MB
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EGAF00001708552 fq.gz 533.2 MB
EGAF00001708553 fq.gz 495.4 MB
EGAF00001708554 fq.gz 482.2 MB
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EGAF00001708556 fq.gz 475.4 MB
EGAF00001708557 fq.gz 554.0 MB
EGAF00001708558 fq.gz 555.6 MB
EGAF00001708559 fq.gz 561.2 MB
EGAF00001708560 fq.gz 560.5 MB
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EGAF00001708562 fq.gz 502.7 MB
EGAF00001708563 fq.gz 609.6 MB
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EGAF00001708566 fq.gz 710.2 MB
EGAF00001708567 fq.gz 715.9 MB
EGAF00001708568 fq.gz 710.8 MB
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EGAF00001708572 fq.gz 595.0 MB
EGAF00001708573 fq.gz 678.4 MB
EGAF00001708574 fq.gz 705.8 MB
EGAF00001708575 fq.gz 704.7 MB
EGAF00001708576 fq.gz 711.9 MB
EGAF00001708577 fq.gz 681.3 MB
EGAF00001708578 fq.gz 698.8 MB
EGAF00001708579 fq.gz 682.1 MB
EGAF00001708580 fq.gz 696.7 MB
EGAF00001708581 fq.gz 574.8 MB
EGAF00001708658 fq.gz 1.2 GB
EGAF00001708659 fq.gz 944.2 MB
EGAF00001708660 fq.gz 1.9 GB
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EGAF00001708662 fq.gz 1.4 GB
EGAF00001708663 fq.gz 952.5 MB
EGAF00001708664 fq.gz 973.7 MB
EGAF00001708665 fq.gz 974.3 MB
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EGAF00001708675 fq.gz 713.8 MB
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EGAF00001708682 fq.gz 850.8 MB
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EGAF00001708684 fq.gz 948.0 MB
EGAF00001708685 fq.gz 942.7 MB
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831 Files (658.0 GB)