Need Help?

BAM files: Brain transcriptome of hereditary cerebral haemorrhage with amyloidosis–Dutch type (HCHWA-D)

cDNA depleted RNA (500ng total RNA input) was fragmented to 150-200 nucleotides in first strand buffer for 3 minutes at 94°C. Random hexamer primed first strand was generated in presence of dATP, dGTP, dCTP and cTTP. Second strand was generated using dUTP instead of dTTP to tag the second strand. Subsequent steps to generate the sequencing libraries were performed with the KAPA HTP Library Preparation Kit for Illumina sequencing with minor modifications, i.e., after indexed adapter ligation to the dsDNA fragments, the library was treated with USER enzyme (NEB_M5505L) in order to digest the second strand derived fragments. After amplification of the libraries, samples with unique sample indexes were pooled and sequenced paired-end 2x50bp on a HiSeq2500 system following standard Illumina guidelines.

Request Access

Policy: Brain Transcriptome of hereditary cerebral haemorrhage with amyloidosis–Dutch type (HCHWA-D)

Controlled access required because the study concerns linked phenotype and genotype data from human subjects which should not be traced back to the patients. Citation plus acknowledgement will be required.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001002730 Other
ID File Type Size Located in
EGAF00001793570 bam 1.9 GB
EGAF00001793571 bam 1.8 GB
EGAF00001793572 bam 1.6 GB
EGAF00001793573 bam 1.5 GB
EGAF00001793574 bam 1.1 GB
EGAF00001793575 bam 1.6 GB
EGAF00001793576 bam 1.5 GB
EGAF00001793577 bam 2.5 GB
EGAF00001793578 bam 1.1 GB
EGAF00001793579 bam 1.8 GB
EGAF00001793580 bam 1.5 GB
EGAF00001793581 bam 1.5 GB
EGAF00001793582 bam 1.6 GB
EGAF00001793583 bam 1.7 GB
EGAF00001793584 bam 1.5 GB
EGAF00001793585 bam 1.8 GB
EGAF00001793586 bam 1.6 GB
EGAF00001793587 bam 1.6 GB
EGAF00001793588 bam 1.6 GB
EGAF00001793589 bam 1.4 GB
EGAF00001793590 bam 1.8 GB
EGAF00001793591 bam 1.8 GB
EGAF00001793592 bam 2.0 GB
EGAF00001793593 bam 1.5 GB
EGAF00001793594 bam 1.9 GB
EGAF00001793595 bam 1.6 GB
EGAF00001793596 bam 1.2 GB
EGAF00001793597 bam 1.2 GB
EGAF00001793598 bam 2.0 GB
EGAF00001793599 bam 2.2 GB
EGAF00001793600 bam 2.4 GB
EGAF00001793601 bam 2.0 GB
EGAF00001793602 bam 1.7 GB
EGAF00001793603 bam 2.0 GB
EGAF00001793604 bam 1.7 GB
EGAF00001793605 bam 1.8 GB
36 Files (60.7 GB)