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Preferential infiltration of unique Vγ9Jγ2‐Vδ2 T cells into glioblastoma multiforme

Glioblastoma multiforme (GBM) is clinically highly aggressive as a result of evolutionary dynamics induced by cross-talk between cancer cells and a heterogeneous group of immune cells in tumor microenvironment. The brain harbors limited numbers of immune cells with few lymphocytes and macrophages; thus, innate‐like lymphocytes, such as γδ T cells, have important roles in antitumor immunity. Here, we characterized GBM‐infiltrating γδ T cells, which may have roles in regulating the GBM tumor microenvironment and cancer cell gene expression. V(D)J repertoires of tumor‐infiltrating and blood‐circulating γδ T cells from four patients were analyzed by next-generation sequencing-based T-cell receptor (TCR) sequencing in addition to mutation and immune profiles in four GBM cases. In all tumor tissues, abundant innate and effector/memory lymphocytes were detected, accompanied by large numbers of tumor‐associated macrophages and closely located tumor‐infiltrating γδ T cells, which appear to have anti-tumor activity. The immune-related gene expression analysis using the TCGA database showed that the signature gene expression extent of γδ T cells were more associated with those of cytotoxic T and Th1 cells and M1 macrophages than those of Th2 cells and M2 macrophages. Although the most abundant γδ T cells were Vγ9Vδ2 T cells in both tumor tissues and blood, the repertoire of intratumoral Vγ9Vδ2 T cells was distinct from that of peripheral blood Vγ9Vδ2 T cells and was dominated by Vγ9Jγ2 sequences, not by canonical Vγ9JγP sequences that are mostly commonly found in blood γδ T cells. Collectively, unique GBM‐specific TCR clonotypes were identified by comparing TCR repertoires of peripheral blood and intra‐tumoral γδ T cells. These findings will be helpful for the elucidation of tumor-specific antigens and development of anticancer immunotherapies using tumor-infiltrating γδ T cells.

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IRCR data access policy generally respects the guidelines of NIH genomic data sharing policy (http://gds.nih.gov/PDF/NIH_GDS_Policy.pdf), but can be overridden by consensual decision of DAC in exceptional cases.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001002790 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00002435182 fastq.gz 882.0 MB
EGAF00002435183 fastq.gz 904.7 MB
EGAF00002435184 fastq.gz 909.2 MB
EGAF00002435185 fastq.gz 933.3 MB
EGAF00002435186 fastq.gz 2.0 GB
EGAF00002435187 fastq.gz 2.0 GB
EGAF00002435188 fastq.gz 3.1 GB
EGAF00002435189 fastq.gz 3.5 GB
EGAF00002435190 fastq.gz 960.0 MB
EGAF00002435191 fastq.gz 977.0 MB
EGAF00002435192 fastq.gz 863.9 MB
EGAF00002435193 fastq.gz 885.9 MB
EGAF00002435194 fastq.gz 738.1 MB
EGAF00002435195 fastq.gz 755.5 MB
EGAF00002435196 fastq.gz 890.4 MB
EGAF00002435197 fastq.gz 907.9 MB
EGAF00002435198 fastq.gz 511.4 MB
EGAF00002435199 fastq.gz 529.1 MB
EGAF00002435200 fastq.gz 2.4 GB
EGAF00002435201 fastq.gz 2.4 GB
EGAF00002435202 fastq.gz 2.8 GB
EGAF00002435203 fastq.gz 2.9 GB
EGAF00002435204 fastq.gz 1.8 GB
EGAF00002435205 fastq.gz 1.8 GB
EGAF00002435206 fastq.gz 2.4 GB
EGAF00002435207 fastq.gz 2.5 GB
EGAF00002435208 fastq.gz 3.6 GB
EGAF00002435209 fastq.gz 3.9 GB
EGAF00002435210 fastq.gz 950.7 MB
EGAF00002435211 fastq.gz 966.6 MB
EGAF00002435212 fastq.gz 781.3 MB
EGAF00002435213 fastq.gz 795.2 MB
EGAF00002435214 fastq.gz 2.3 GB
EGAF00002435215 fastq.gz 2.3 GB
EGAF00002435216 fastq.gz 2.8 GB
EGAF00002435217 fastq.gz 3.0 GB
36 Files (62.8 GB)