Gene expression by RNAseq in bronchial biopsies of asthmatics, asthma in remission and healthy subjects

Dataset ID Technology Samples
EGAD00001005112 Illumina HiSeq 2500 184

Dataset Description

The study focus was differential expression in bronchial biopsies between persistent asthma, asthma in remission and healthy controls using RNAseq. There were 184 samples that passed QC. RNA samples were processed using the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina, San Diego, CA), using an automated procedure in a Caliper Sciclone NGS Workstation (PerkinElmer, Waltham, MA). In this procedure, all cytoplasmic and mitochondria rRNA was removed (RiboZero Gold kit). The obtained cDNA fragment libraries were loaded in pools of multiple samples unto an Illumina HiSeq2500 sequencer using default parameters for paired-end sequencing (2 × 100 bp). Data are available as 221 pairs of FASTQ-files. Note that several samples are associated with multiple sequence runs.

Who controls access to this dataset

For each dataset that requires controlled access, there is a corresponding Data Access Committee (DAC) who determine access permissions. Access to actual data files is not managed by the EGA. If you need to request access to this data set, please contact:

Asthma Remission Study Data Access Committee
Contact person: Maarten van den Berge
Email: m [dot] van [dot] den [dot] berge [at] umcg [dot] nl
More details: EGAC00001001258


You don't have access to the download section.