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CyTOF of 27 DLBCLs

Diffuse large B-cell lymphoma (DLBCL) is the most common histologic subtype of non-Hodgkin lymphoma and is notorious for its clinical heterogeneity. Patient outcomes can be predicted by cell-of-origin (COO) classification, demonstrating that the underlying transcriptional signature of malignant B-cells informs biological behavior in the context of standard combination chemotherapy regimens. In the current study, we used mass cytometry (CyTOF) to examine tumor phenotypes at the protein level with single cell resolution in a collection of 27 diagnostic DLBCL biopsy specimens from treatment naïve patients. We found that malignant B-cells from each patient occupied unique regions in 37-dimensional phenotypic space with no apparent clustering of samples into discrete subtypes. Interestingly, variable MHC class II expression was found to be the greatest contributor to phenotypic diversity. Within individual tumors, a subset of cases showed multiple phenotypic subpopulations, and in one case we were able to demonstrate direct correspondence between protein-level phenotypic subsets and DNA mutation-defined subclones. In summary, CyTOF analysis can resolve both inter- and intra-tumoral heterogeneity among primary samples, and reveals that each case of DLBCL is unique and may be comprised of multiple, genetically distinct subclones.

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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001003860 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00002799076 bam 129.0 MB
EGAF00002799077 bam 97.5 MB
EGAF00002799078 bam 126.1 MB
EGAF00002799079 bam 125.5 MB
EGAF00002799080 bam 127.2 MB
EGAF00002799081 bam 22.4 MB
EGAF00002799082 bam 14.8 MB
EGAF00002799083 bam 13.4 MB
EGAF00002799084 bam 15.0 MB
EGAF00002799085 bam 208.4 MB
EGAF00002799086 bam 247.6 MB
EGAF00002799087 bam 230.0 MB
EGAF00002799088 bam 247.5 MB
EGAF00002799089 bam 190.7 MB
EGAF00002799090 bam 3.2 GB
EGAF00002799091 bam 2.7 GB
EGAF00002799092 bam 2.8 GB
17 Files (10.5 GB)