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Single MII oocyte mRNA expression from women

This dataset contains 14 paired-end FASTQ sequences from mRNA-Seq on single human M-II stage oocytes that were collected from ovarian tissue from unstimulated patients undergoing fertility preservation treatments due to cancer diagnoses, which did not influence ovarian function. Cumulus-oocyte-complexes were matured in vitro according Gruhn et al (Science 365: 1466-1469) and short term flash frozen prior to lysis, RNA extraction, full length cDNA preparation and amplification using the Ultra-low-input SMART-Seq2 v4 kit from Takara Clonetech. Further, these cDNA were used to prepare libraries for sequencing according the Nextera XT DNA library preparation kit from Illumina

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Access is granted according to Danish National ethics regulation via application to the Videnskabsetisk Committee and will comply with Danish law and GDPR

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001004220 Transcriptome Analysis

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
EGAF00003446565 fastq.gz 1.6 GB Report
EGAF00003446566 fastq.gz 1.6 GB Report
EGAF00003446567 fastq.gz 840.5 MB Report
EGAF00003446568 fastq.gz 874.9 MB Report
EGAF00003446569 fastq.gz 1.5 GB Report
EGAF00003446570 fastq.gz 1.6 GB Report
EGAF00003446571 fastq.gz 1.5 GB Report
EGAF00003446572 fastq.gz 1.5 GB Report
EGAF00003446573 fastq.gz 1.3 GB Report
EGAF00003446574 fastq.gz 1.4 GB Report
EGAF00003446575 fastq.gz 1.1 GB Report
EGAF00003446576 fastq.gz 1.1 GB Report
EGAF00003446577 fastq.gz 1.4 GB Report
EGAF00003446578 fastq.gz 1.4 GB Report
EGAF00003446579 fastq.gz 1.4 GB Report
EGAF00003446580 fastq.gz 1.4 GB Report
EGAF00003446581 fastq.gz 1.3 GB Report
EGAF00003446582 fastq.gz 1.3 GB Report
EGAF00003446583 fastq.gz 1.3 GB Report
EGAF00003446584 fastq.gz 1.3 GB Report
EGAF00003446585 fastq.gz 1.1 GB Report
EGAF00003446586 fastq.gz 1.1 GB Report
EGAF00003446587 fastq.gz 1.5 GB Report
EGAF00003446588 fastq.gz 1.5 GB Report
EGAF00003446589 fastq.gz 1.4 GB Report
EGAF00003446590 fastq.gz 1.4 GB Report
EGAF00003446591 fastq.gz 1.2 GB Report
EGAF00003446592 fastq.gz 1.3 GB Report
28 Files (37.3 GB)