Single MII oocyte mRNA expression from women
This dataset contains 14 paired-end FASTQ sequences from mRNA-Seq on single human M-II stage oocytes that were collected from ovarian tissue from unstimulated patients undergoing fertility preservation treatments due to cancer diagnoses, which did not influence ovarian function. Cumulus-oocyte-complexes were matured in vitro according Gruhn et al (Science 365: 1466-1469) and short term flash frozen prior to lysis, RNA extraction, full length cDNA preparation and amplification using the Ultra-low-input SMART-Seq2 v4 kit from Takara Clonetech. Further, these cDNA were used to prepare libraries for sequencing according the Nextera XT DNA library preparation kit from Illumina
- 14 samples
- DAC: EGAC00001001473
- Technology: NextSeq 500
Access is granted according to Danish National ethics regulation via application to the Videnskabsetisk Committee
Access is granted according to Danish National ethics regulation via application to the Videnskabsetisk Committee and will comply with Danish law and GDPR
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| EGAS00001004220 | Transcriptome Analysis |
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