Dataset

RNA sequencing data for Molecular Characterization of ETMRs

Dataset ID Technology Samples
EGAD00001006209 NextSeq 500 2

Dataset Description

The dataset contains two samples from one patient.
As a representative FFPE tissue sample, ET174 was histologically iden-
tified, targeted and microdissected with a puncher for nucleic acid
extraction. RNA was extracted using the automated Maxwell
system with the Maxwell 16 LEV RNA FFPE Kit (Promega), according to
the manufacturer’s instructions. To evaluate FFPE RNA quality, we used
the percentage of RNA fragments >200 nt fragment determination
value (DV200). Only RNA samples with DV200 > 70% were included for
sequencing on a NextSeq 500 (Illumina). Eight lanes have been sequenced
resulting in 16 Fastq files (paired end).

Who controls access to this dataset

For each dataset that requires controlled access, there is a corresponding Data Access Committee (DAC) who determine access permissions. Access to actual data files is not managed by the EGA. If you need to request access to this data set, please contact:

Data Access Committee of divisions B060 and B062, German Cancer Research Center (DKFZ)
Contact person: DACO B060x
Email: daco-dkfz-b06x [at] dkfz-heidelberg [dot] de
More details: EGAC00001001626

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