Dataset

miRNA-seq data for Molecular Characterization of ETMR

Dataset ID Technology Samples
EGAD00001006218 Illumina HiSeq 2000 10

Dataset Description

This dataset contains miRNA-seq data from 10 patients.
Small RNAs were isolated as described previously57,58 from fresh-frozen
tumour material. In brief, total RNA was extracted using guanidinium
isothiocyanate/phenol extraction followed by 3′-adaptor ligation of
barcoded adenylated adaptors. Samples were pooled in two sets of five
samples. Subsequently, gel electrophoresis was used to isolate small
RNAs (19–35 nt) and purified using ethanol precipitation. Fragments
were then amplified using standard PCR, isolated using gel electropho-
resis and purified using ethanol precipitation. Samples were sequenced
on a HiSeq 2000 v.4 machine resulting in 10 Fastq files.

Who controls access to this dataset

For each dataset that requires controlled access, there is a corresponding Data Access Committee (DAC) who determine access permissions. Access to actual data files is not managed by the EGA. If you need to request access to this data set, please contact:

Data Access Committee of divisions B060 and B062, German Cancer Research Center (DKFZ)
Contact person: DACO B060x
Email: daco-dkfz-b06x [at] dkfz-heidelberg [dot] de
More details: EGAC00001001626

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