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Smart-seq 2 single cell sequencing of CD4 and CD8 T cells from the blood and synovial fluid of 4 psoriatic arthritis patients.

This dataset was used to characterise T cell gene expression and clonality at sites of active inflammation within the joints of psoriatic arthritis (PsA) patients, and to compare these results with T cells from the peripheral blood of those same patients. Freshly sorted CD45RA negative CD3+CD4+ and CD3+CD8+ single cells from four patients were individually flow sorted into 96-well full-skirted plates (Eppendorf) containing 10µL of a 2% Dithiothreitol (DTT, 2M Sigma-Aldrich), RTL lysis buffer (Qiagen) solution. Cell lysates were sealed, mixed and spun down before storing at -80 ºC. Paired-end multiplexed sequencing libraries were prepared following the Smart-seq 2 protocol using the Nextera XT DNA library prep kit (Illumina). A pool of barcoded libraries from four different plates were sequenced across two lanes on the Illumina HiSeq 2500.

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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001002104 Cancer Genomics

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00004188083 cram 4.1 MB
EGAF00004188084 cram 9.3 MB
EGAF00004188085 cram 34.2 MB
EGAF00004188086 cram 40.9 MB
EGAF00004188087 cram 28.6 MB
EGAF00004188088 cram 69.6 MB
EGAF00004188089 cram 60.6 MB
EGAF00004188090 cram 69.6 MB
EGAF00004188091 cram 59.4 MB
EGAF00004188092 cram 95.3 kB
EGAF00004188093 cram 82.9 MB
EGAF00004188094 cram 68.0 MB
EGAF00004188095 cram 75.4 MB
EGAF00004188096 cram 67.9 MB
EGAF00004188097 cram 52.6 MB
EGAF00004188098 cram 30.1 kB
EGAF00004188099 cram 68.9 MB
EGAF00004188100 cram 43.6 MB
EGAF00004188101 cram 53.4 MB
EGAF00004188102 cram 66.8 MB
EGAF00004188103 cram 45.1 MB
EGAF00004188104 cram 61.2 kB
EGAF00004188105 cram 85.8 MB
EGAF00004188106 cram 80.2 MB
EGAF00004188107 cram 80.2 MB
EGAF00004188108 cram 61.3 MB
EGAF00004188109 cram 61.0 MB
EGAF00004188110 cram 40.7 MB
EGAF00004188111 cram 53.5 MB
EGAF00004188112 cram 40.9 MB
EGAF00004188113 cram 50.7 kB
EGAF00004188114 cram 61.9 MB
EGAF00004188115 cram 42.4 MB
EGAF00004188116 cram 370.3 kB
EGAF00004188117 cram 67.0 MB
EGAF00004188118 cram 73.1 MB
EGAF00004188119 cram 71.7 MB
EGAF00004188120 cram 64.1 MB
EGAF00004188121 cram 46.4 MB
EGAF00004188122 cram 54.8 MB
EGAF00004188123 cram 49.3 MB
EGAF00004188124 cram 46.3 MB
EGAF00004188125 cram 46.1 MB
EGAF00004188126 cram 52.6 MB
EGAF00004188127 cram 52.8 MB
EGAF00004188128 cram 57.0 MB
EGAF00004188129 cram 251.1 kB
EGAF00004188130 cram 63.8 MB
EGAF00004188131 cram 67.6 MB
EGAF00004188132 cram 55.3 MB
EGAF00004188133 cram 59.8 MB
EGAF00004188134 cram 37.5 MB
EGAF00004188135 cram 49.6 MB
EGAF00004188136 cram 42.4 MB
EGAF00004188137 cram 44.7 MB
EGAF00004188138 cram 43.2 MB
EGAF00004188139 cram 53.2 MB
EGAF00004188140 cram 209.8 kB
EGAF00004188141 cram 35.8 MB
EGAF00004188142 cram 544.0 kB
EGAF00004188143 cram 75.1 MB
EGAF00004188144 cram 69.7 MB
EGAF00004188145 cram 52.2 MB
EGAF00004188146 cram 41.4 MB
EGAF00004188147 cram 67.8 MB
EGAF00004188148 cram 57.2 MB
EGAF00004188149 cram 49.4 MB
EGAF00004188150 cram 76.1 MB
EGAF00004188151 cram 68.6 MB
EGAF00004188152 cram 2.4 MB
EGAF00004188153 cram 56.8 MB
EGAF00004188154 cram 68.3 MB
EGAF00004188155 cram 81.7 MB
EGAF00004188156 cram 75.2 MB
EGAF00004188157 cram 78.7 MB
EGAF00004188158 cram 50.6 MB
EGAF00004188159 cram 69.1 MB
EGAF00004188160 cram 85.7 MB
EGAF00004188161 cram 8.1 MB
EGAF00004188162 cram 81.6 MB
EGAF00004188163 cram 32.9 MB
EGAF00004188164 cram 48.3 MB
EGAF00004188165 cram 4.0 MB
EGAF00004188166 cram 6.7 MB
EGAF00004188167 cram 58.2 MB
EGAF00004188168 cram 23.9 kB
EGAF00004188169 cram 51.3 MB
EGAF00004188170 cram 59.0 MB
EGAF00004188171 cram 69.7 MB
EGAF00004188172 cram 62.4 MB
EGAF00004188173 cram 68.6 MB
EGAF00004188174 cram 33.6 MB
EGAF00004188175 cram 22.0 MB
EGAF00004188176 cram 12.8 MB
EGAF00004188177 cram 43.7 kB
EGAF00004188178 cram 24.4 kB
EGAF00004188179 cram 279.6 kB
EGAF00004188180 cram 651.1 kB
EGAF00004188181 cram 2.4 MB
EGAF00004188182 cram 1.7 MB
EGAF00004188183 cram 1.1 MB
EGAF00004188184 cram 20.1 MB
EGAF00004188185 cram 2.3 MB
EGAF00004188186 cram 17.6 MB
EGAF00004188187 cram 619.0 kB
EGAF00004188188 cram 15.9 MB
EGAF00004188189 cram 733.6 kB
EGAF00004188190 cram 23.2 MB
EGAF00004188191 cram 1.8 MB
EGAF00004188192 cram 40.4 MB
EGAF00004188193 cram 946.6 kB
EGAF00004188194 cram 23.2 MB
EGAF00004188195 cram 476.6 kB
EGAF00004188196 cram 23.6 MB
EGAF00004188197 cram 28.0 MB
EGAF00004188198 cram 1.4 MB
EGAF00004188199 cram 63.3 kB
EGAF00004188200 cram 38.3 kB
EGAF00004188201 cram 93.6 kB
EGAF00004188202 cram 87.5 kB
EGAF00004188203 cram 27.7 MB
EGAF00004188204 cram 172.0 kB
EGAF00004188205 cram 10.0 MB
EGAF00004188206 cram 121.6 kB
EGAF00004188207 cram 22.1 MB
EGAF00004188208 cram 163.5 kB
EGAF00004188209 cram 20.0 MB
EGAF00004188210 cram 115.5 kB
EGAF00004188211 cram 23.7 MB
EGAF00004188212 cram 230.3 kB
EGAF00004188213 cram 21.7 MB
EGAF00004188214 cram 274.3 kB
EGAF00004188215 cram 10.7 MB
EGAF00004188216 cram 280.6 kB
EGAF00004188217 cram 14.1 MB
EGAF00004188218 cram 504.2 kB
EGAF00004188219 cram 18.0 MB
EGAF00004188220 cram 424.2 kB
EGAF00004188221 cram 8.3 MB
EGAF00004188222 cram 378.7 kB
EGAF00004188223 cram 80.7 kB
EGAF00004188224 cram 297.0 kB
EGAF00004188225 cram 6.8 MB
EGAF00004188226 cram 42.0 MB
EGAF00004188227 cram 22.4 MB
EGAF00004188228 cram 21.3 MB
EGAF00004188229 cram 32.7 MB
EGAF00004188230 cram 31.9 MB
EGAF00004188231 cram 42.1 MB
EGAF00004188232 cram 27.1 MB
EGAF00004188233 cram 37.7 MB
EGAF00004188234 cram 31.0 MB
EGAF00004188235 cram 40.0 MB
EGAF00004188236 cram 41.3 MB
EGAF00004188237 cram 31.8 MB
EGAF00004188238 cram 49.2 MB
EGAF00004188239 cram 30.4 MB
EGAF00004188240 cram 43.0 MB
EGAF00004188241 cram 35.9 MB
EGAF00004188242 cram 30.6 MB
EGAF00004188243 cram 41.3 MB
EGAF00004188244 cram 39.8 MB
EGAF00004188245 cram 44.8 MB
EGAF00004188246 cram 33.9 MB
EGAF00004188247 cram 18.9 MB
EGAF00004188248 cram 929.4 kB
EGAF00004188249 cram 8.8 MB
EGAF00004188250 cram 114.5 kB
EGAF00004188251 cram 14.5 MB
EGAF00004188252 cram 152.5 kB
EGAF00004188253 cram 35.0 MB
EGAF00004188254 cram 215.2 kB
EGAF00004188255 cram 37.9 MB
EGAF00004188256 cram 317.7 kB
EGAF00004188257 cram 47.4 MB
EGAF00004188258 cram 271.5 kB
EGAF00004188259 cram 34.7 MB
EGAF00004188260 cram 344.5 kB
EGAF00004188261 cram 38.2 MB
EGAF00004188262 cram 12.1 MB
EGAF00004188263 cram 25.5 MB
EGAF00004188264 cram 44.3 MB
EGAF00004188265 cram 34.7 MB
EGAF00004188266 cram 56.6 MB
EGAF00004188267 cram 62.9 MB
EGAF00004188268 cram 43.5 MB
EGAF00004188269 cram 39.4 MB
EGAF00004188270 cram 29.0 MB
EGAF00004188271 cram 10.3 MB
EGAF00004188272 cram 1.4 MB
EGAF00004188273 cram 13.9 MB
EGAF00004188274 cram 7.4 MB
EGAF00004188275 cram 38.1 MB
EGAF00004188276 cram 29.4 MB
EGAF00004188277 cram 49.5 MB
EGAF00004188278 cram 25.3 MB
EGAF00004188279 cram 49.5 MB
EGAF00004188280 cram 32.6 MB
EGAF00004188281 cram 74.2 MB
EGAF00004188282 cram 40.6 MB
EGAF00004188283 cram 50.6 MB
EGAF00004188284 cram 46.6 MB
EGAF00004188285 cram 33.1 MB
EGAF00004188286 cram 59.2 MB
EGAF00004188287 cram 31.1 MB
EGAF00004188288 cram 36.8 MB
EGAF00004188289 cram 28.6 MB
EGAF00004188290 cram 33.8 MB
EGAF00004188291 cram 58.5 MB
EGAF00004188292 cram 36.6 MB
EGAF00004188293 cram 48.2 MB
EGAF00004188294 cram 36.7 MB
EGAF00004188295 cram 29.7 MB
EGAF00004188296 cram 30.5 MB
EGAF00004188297 cram 6.0 MB
EGAF00004188298 cram 192.5 kB
EGAF00004188299 cram 26.4 MB
EGAF00004188300 cram 201.0 kB
EGAF00004188301 cram 28.7 MB
EGAF00004188302 cram 181.1 kB
EGAF00004188303 cram 56.7 MB
EGAF00004188304 cram 363.6 kB
EGAF00004188305 cram 38.2 MB
EGAF00004188306 cram 32.2 MB
EGAF00004188307 cram 29.2 MB
EGAF00004188308 cram 18.1 MB
EGAF00004188309 cram 33.0 MB
EGAF00004188310 cram 28.2 MB
EGAF00004188311 cram 24.0 MB
EGAF00004188312 cram 39.0 MB
EGAF00004188313 cram 23.5 MB
EGAF00004188314 cram 29.4 MB
EGAF00004188315 cram 34.0 MB
EGAF00004188316 cram 51.1 MB
EGAF00004188317 cram 30.3 MB
EGAF00004188318 cram 88.7 MB
EGAF00004188319 cram 21.6 MB
EGAF00004188320 cram 32.8 MB
EGAF00004188321 cram 14.2 kB
EGAF00004188322 cram 10.3 kB
EGAF00004188323 cram 16.2 kB
EGAF00004188324 cram 14.8 kB
EGAF00004188325 cram 15.5 kB
EGAF00004188326 cram 14.8 kB
EGAF00004188327 cram 13.5 kB
EGAF00004188328 cram 14.7 kB
EGAF00004188329 cram 16.3 kB
EGAF00004188330 cram 16.3 kB
EGAF00004188331 cram 14.7 kB
EGAF00004188332 cram 14.7 kB
EGAF00004188333 cram 15.0 kB
EGAF00004188334 cram 16.1 kB
EGAF00004188335 cram 14.5 kB
EGAF00004188336 cram 16.8 kB
EGAF00004188337 cram 14.4 kB
EGAF00004188338 cram 15.9 kB
EGAF00004188339 cram 15.3 kB
EGAF00004188340 cram 14.9 kB
EGAF00004188341 cram 16.1 kB
EGAF00004188342 cram 15.0 kB
EGAF00004188343 cram 13.4 kB
EGAF00004188344 cram 14.1 kB
EGAF00004188345 cram 15.4 MB
EGAF00004188346 cram 234.9 kB
EGAF00004188347 cram 26.9 MB
EGAF00004188348 cram 337.6 kB
EGAF00004188349 cram 38.2 MB
EGAF00004188350 cram 4.0 MB
EGAF00004188351 cram 35.5 MB
EGAF00004188352 cram 32.6 MB
EGAF00004188353 cram 29.8 MB
EGAF00004188354 cram 50.4 MB
EGAF00004188355 cram 44.9 MB
EGAF00004188356 cram 32.5 MB
EGAF00004188357 cram 27.3 MB
EGAF00004188358 cram 44.7 MB
EGAF00004188359 cram 26.4 MB
EGAF00004188360 cram 49.9 MB
EGAF00004188361 cram 32.9 MB
EGAF00004188362 cram 40.5 MB
EGAF00004188363 cram 33.7 MB
EGAF00004188364 cram 49.4 MB
EGAF00004188365 cram 31.5 MB
EGAF00004188366 cram 33.8 MB
EGAF00004188367 cram 10.2 MB
EGAF00004188368 cram 38.2 MB
EGAF00004188369 cram 10.5 MB
EGAF00004188370 cram 8.9 MB
EGAF00004188371 cram 26.6 MB
EGAF00004188372 cram 12.4 MB
EGAF00004188373 cram 13.4 MB
EGAF00004188374 cram 22.1 MB
EGAF00004188375 cram 22.4 MB
EGAF00004188376 cram 14.4 MB
EGAF00004188377 cram 41.7 MB
EGAF00004188378 cram 15.9 MB
EGAF00004188379 cram 25.3 MB
EGAF00004188380 cram 23.9 MB
EGAF00004188381 cram 21.5 MB
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EGAF00004188383 cram 20.8 MB
EGAF00004188384 cram 26.3 MB
EGAF00004188385 cram 19.2 MB
EGAF00004188386 cram 24.4 MB
EGAF00004188387 cram 13.3 MB
EGAF00004188388 cram 18.2 MB
EGAF00004188389 cram 24.1 MB
EGAF00004188390 cram 18.8 MB
EGAF00004188391 cram 24.0 MB
EGAF00004188392 cram 21.5 MB
EGAF00004188393 cram 13.1 MB
EGAF00004188394 cram 216.1 kB
EGAF00004188395 cram 22.7 MB
EGAF00004188396 cram 229.8 kB
EGAF00004188397 cram 30.9 MB
EGAF00004188398 cram 25.0 MB
EGAF00004188399 cram 32.7 MB
EGAF00004188400 cram 33.6 MB
EGAF00004188401 cram 26.5 MB
EGAF00004188402 cram 29.3 MB
EGAF00004188403 cram 27.8 MB
EGAF00004188404 cram 27.0 MB
EGAF00004188405 cram 30.7 MB
EGAF00004188406 cram 37.7 MB
EGAF00004188407 cram 21.9 MB
EGAF00004188408 cram 33.1 MB
EGAF00004188409 cram 28.7 MB
EGAF00004188410 cram 43.4 MB
EGAF00004188411 cram 15.2 MB
EGAF00004188412 cram 77.5 kB
EGAF00004188413 cram 32.3 MB
EGAF00004188414 cram 50.2 MB
EGAF00004188415 cram 25.3 MB
EGAF00004188416 cram 2.6 MB
EGAF00004188417 cram 13.9 kB
EGAF00004188418 cram 9.4 kB
EGAF00004188419 cram 15.0 kB
EGAF00004188420 cram 14.0 kB
EGAF00004188421 cram 17.7 kB
EGAF00004188422 cram 18.8 kB
EGAF00004188423 cram 18.3 kB
EGAF00004188424 cram 20.4 kB
EGAF00004188425 cram 18.0 kB
EGAF00004188426 cram 18.9 kB
EGAF00004188427 cram 20.1 kB
EGAF00004188428 cram 22.6 kB
EGAF00004188429 cram 17.1 kB
EGAF00004188430 cram 19.3 kB
EGAF00004188431 cram 17.4 kB
EGAF00004188432 cram 25.0 kB
EGAF00004188433 cram 18.8 kB
EGAF00004188434 cram 22.9 kB
EGAF00004188435 cram 19.0 kB
EGAF00004188436 cram 17.1 kB
EGAF00004188437 cram 18.2 kB
EGAF00004188438 cram 21.2 kB
EGAF00004188439 cram 14.7 kB
EGAF00004188440 cram 16.1 kB
EGAF00004188441 cram 7.0 MB
EGAF00004188442 cram 176.3 kB
EGAF00004188443 cram 22.2 MB
EGAF00004188444 cram 1.7 MB
EGAF00004188445 cram 23.1 MB
EGAF00004188446 cram 30.3 MB
EGAF00004188447 cram 26.2 MB
EGAF00004188448 cram 35.9 MB
EGAF00004188449 cram 37.9 MB
EGAF00004188450 cram 35.7 MB
EGAF00004188451 cram 34.3 MB
EGAF00004188452 cram 47.1 MB
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EGAF00004188454 cram 37.4 MB
EGAF00004188455 cram 26.2 MB
EGAF00004188456 cram 68.4 MB
EGAF00004188457 cram 27.0 MB
EGAF00004188458 cram 55.7 MB
EGAF00004188459 cram 30.3 MB
EGAF00004188460 cram 21.7 MB
EGAF00004188461 cram 31.9 MB
EGAF00004188462 cram 38.7 MB
EGAF00004188463 cram 5.9 MB
EGAF00004188464 cram 16.4 MB
EGAF00004188465 cram 6.4 MB
EGAF00004188466 cram 31.8 MB
EGAF00004188467 cram 19.5 MB
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EGAF00004188469 cram 35.3 MB
EGAF00004188470 cram 20.2 MB
EGAF00004188471 cram 45.7 MB
EGAF00004188472 cram 30.3 MB
EGAF00004188473 cram 39.1 MB
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EGAF00004188475 cram 30.8 MB
EGAF00004188476 cram 30.3 MB
EGAF00004188477 cram 27.7 MB
EGAF00004188478 cram 48.9 MB
EGAF00004188479 cram 32.6 MB
EGAF00004188480 cram 38.0 MB
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EGAF00004188485 cram 40.7 MB
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EGAF00004188488 cram 42.0 MB
EGAF00004188489 cram 10.5 MB
EGAF00004188490 cram 178.4 kB
EGAF00004188491 cram 36.5 MB
EGAF00004188492 cram 16.8 MB
EGAF00004188493 cram 29.8 MB
EGAF00004188494 cram 91.1 MB
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EGAF00004188497 cram 40.2 MB
EGAF00004188498 cram 45.0 MB
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EGAF00004188501 cram 25.8 MB
EGAF00004188502 cram 19.6 MB
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EGAF00004188505 cram 58.9 MB
EGAF00004188506 cram 66.3 MB
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EGAF00004188510 cram 67.2 MB
EGAF00004188511 cram 592.3 kB
EGAF00004188512 cram 13.9 MB
EGAF00004188513 cram 1.4 MB
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EGAF00004188529 cram 89.0 MB
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EGAF00004188533 cram 125.6 MB
EGAF00004188534 cram 188.1 MB
EGAF00004188535 cram 379.0 kB
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EGAF00004188545 cram 22.6 MB
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EGAF00004188549 cram 16.7 MB
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EGAF00004188552 cram 7.1 MB
EGAF00004188553 cram 88.0 MB
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EGAF00004188557 cram 43.6 MB
EGAF00004188558 cram 484.4 kB
EGAF00004188559 cram 272.0 kB
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EGAF00004188563 cram 249.3 kB
EGAF00004188564 cram 624.1 kB
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EGAF00004188568 cram 19.0 MB
EGAF00004188569 cram 2.2 MB
EGAF00004188570 cram 16.7 MB
EGAF00004188571 cram 586.5 kB
EGAF00004188572 cram 15.0 MB
EGAF00004188573 cram 716.8 kB
EGAF00004188574 cram 22.0 MB
EGAF00004188575 cram 1.7 MB
EGAF00004188576 cram 38.4 MB
EGAF00004188577 cram 901.7 kB
EGAF00004188578 cram 21.8 MB
EGAF00004188579 cram 450.1 kB
EGAF00004188580 cram 22.3 MB
EGAF00004188581 cram 26.3 MB
EGAF00004188582 cram 1.3 MB
EGAF00004188583 cram 64.5 kB
EGAF00004188584 cram 34.0 kB
EGAF00004188585 cram 87.4 kB
EGAF00004188586 cram 84.5 kB
EGAF00004188587 cram 26.1 MB
EGAF00004188588 cram 165.3 kB
EGAF00004188589 cram 9.4 MB
EGAF00004188590 cram 113.3 kB
EGAF00004188591 cram 20.9 MB
EGAF00004188592 cram 165.6 kB
EGAF00004188593 cram 18.9 MB
EGAF00004188594 cram 125.5 kB
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