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Resequencing candidate genes for male spermatogenic impairment

This study aims to identify novel candidate variants from human Y-chromosomal genes DAZ, BPY2 and CDY1/2 by resequencing the coding regions of these genes from male patients with spermatogenic impairment. The coding regions of the genes plus a selection of phylogenetically informative Y-chromosomal markers have been amplified by standard PCR, amplicon lengths range from 178 to 486 bp. Amplicons were quantified by gel electrophoresis and pooled in approx. equimolar concentrations per patient. For each of the 480 submitted samples, approx. 1 microgram of amplified DNA pool was provided in a total volume of 120 microlitres. The samples were indexed and libraries prepared for PE250bp Illumina MiSeq runs. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/ .

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Study ID Study Title Study Type
EGAS00001002157 Resequencing

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
EGAF00003441505 cram 3.6 MB Report
EGAF00003441506 cram 3.9 MB Report
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EGAF00003441933 cram 5.3 MB Report
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EGAF00003441996 cram 4.2 MB Report
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EGAF00003442005 cram 3.6 MB Report
EGAF00003442006 cram 3.7 MB Report
EGAF00003442007 cram 3.9 MB Report
EGAF00003442008 cram 4.1 MB Report
EGAF00003442009 cram 4.8 MB Report
EGAF00003442010 cram 4.0 MB Report
EGAF00003442011 cram 4.9 MB Report
EGAF00003442012 cram 3.5 MB Report
EGAF00003442013 cram 4.0 MB Report
EGAF00003442014 cram 4.5 MB Report
EGAF00003442015 cram 3.7 MB Report
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EGAF00003442017 cram 3.7 MB Report
EGAF00003442018 cram 4.0 MB Report
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EGAF00003442025 cram 4.1 MB Report
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EGAF00004950635 cram 15.6 MB Report
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EGAF00004950637 cram 16.9 MB Report
480 Files (3.0 GB)