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Single-cell gene expression data from CD8+ T cells from two Austrian COVID19 patients stimulated with wildtype and mutant SARS-Cov-2 peptides

The data contains single-cell gene sequencing data (10x Genomics) from FACS-purified CD8 T lymphocytes from two Austrian patients. The cells were stimulated with one MHC class I peptides obtained from a common (wild type) variant and an emerging mutant variant of the SARS-Cov-2 virus. Then the samples were multiplexed using hashtag oligos. We provide the raw and aligned sequence data for: i. The single-cell experiments ii. The PCR-amplified samples for enrichment of the hashtag oligo multiplexing barcodes iii. The PCR-amplified samples for enrichment of the T Cell Receptor (TCR) VDJ region for immuno-profiling. The samples and libraries were processed and obtained in collaboration between St. Anna Children's Cancer Research Institute (CCRI), CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, and the Medical University of Vienna. The cell barcodes and processed data has been submitted to the GEO database with GEO accession GSE166651.

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Terms and conditions to authorize access to the raw sequence data from patients in this study.

Terms and Conditions: In signing this Agreement: 1. You agree to use the Data only for research in the areas of biology and medicine. 2. You agree to preserve, at all times, the confidentiality of information and Data pertaining to Data Subjects. In particular, You undertake not to use, or attempt to use the Data to compromise or otherwise infringe the confidentiality of information on Data Subjects and their right to privacy. 3. You agree not to transfer or disclose the Data, in whole or part, or any identifiable material derived from the Data, to others, except as necessary for data/safety monitoring or programme management. Should You wish to share the Data with a collaborator outside the same Institution, the third party must make a separate application for access to the Data. 4. You agree to use the data for the approved purpose and project described in your application; use of the data for a new purpose or project will require a new application and a new approval. 5. You accept that the providers and the funders of the Data or any part of the Data: a) bear no legal responsibility for the accuracy or comprehensiveness of the Data; and b) accept no liability for indirect, consequential, or incidental, damages or losses arising from use of the Data, or from the unavailability of, or break in access to, the Data for whatever reason. 6. You understand that duplication, except as reasonably required to carry out Your research with the Data, or sale of all or part of the Data on any media is not be permitted. 7. You accept that this agreement will terminate immediately upon any breach of this agreement by You. Upon termination You will be required to promptly destroy any Data held. 8. You accept that it may be necessary for the Data Provider or its appointed agents to alter the terms of this agreement from time to time in order to address new concerns. In this event, the EGA or its appointed agent will contact You to inform You of any changes and You agree that Your continued use of the Data shall be dependent on the parties entering into a new version of the Agreement. 9. You accept that the Data may be protected by and subject to laws and regulations, including but not limited to applicable laws on the protection of personal data, and that You are responsible for ensuring compliance with any such applicable law. You undertake to implement or have implemented adequate technical and organizational security measures to protect the Data.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001005060 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00005067552 fastq.gz 1.6 GB
EGAF00005067553 fastq.gz 1.7 GB
EGAF00005067554 fastq.gz 14.3 GB
EGAF00005067555 fastq.gz 14.5 GB
EGAF00005067556 fastq.gz 1.4 GB
EGAF00005067557 fastq.gz 1.3 GB
EGAF00005067558 fastq.gz 1.7 GB
EGAF00005067559 fastq.gz 1.7 GB
EGAF00005067560 bam 2.2 GB
EGAF00005067561 bam 1.5 MB
EGAF00005067562 bam 2.3 GB
EGAF00005067563 fastq.gz 1.5 GB
EGAF00005067564 fastq.gz 1.4 GB
EGAF00005067565 fastq.gz 14.3 GB
EGAF00005067566 fastq.gz 14.0 GB
EGAF00005067567 bam 1.5 MB
EGAF00005067568 bam 29.4 GB
EGAF00005067569 bam 28.8 GB
18 Files (132.0 GB)