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Precise reconstruction of the tumor microenvironment using bulk RNA-seq and a unique machine learning-based algorithm trained on artificial transcriptomes

Cellular deconvolution algorithms virtually reconstruct tissue composition by analyzing the gene expression of complex tissues. Here, we present the decision tree machine learning algorithm, Kassandra, trained on a broad collection of > 9,400 tissue and blood sorted cell RNA profiles to accurately reconstruct the tumor microenvironment (TME). Bioinformatic correction for technical and biological variability, aberrant cancer cell expression inclusion, and the accurate quantification and normalization of transcript expression increased the stability and robustness of Kassandra. Performance was validated on over 4,000 H&E tissue slides and more than 1,000 normal and tumor tissues by comparison with cytometric, immunohistochemical or single-cell RNA-seq measurements. Kassandra accurately deconvolved stromal and immune elements of blood and tumors, revealing the role of the TME in tumor pathogenesis. Digital TME reconstruction revealed that the presence of PD1-positive CD8+ T cells strongly correlated with immunotherapy response and increased the predictive potential of established biomarkers, indicating that Kassandra could potentially be utilized in future clinical applications.

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Terms for provision of data to third parties

DATA ACCESS AGREEMENT These terms and conditions govern access to the managed access datasets (details of which are set out in Appendix I) to which the User Institution has requested access. The User Institution agrees to be bound by these terms and conditions. Definitions Authorized Personnel: The individuals at the User Institution to whom BostonGene Corporation grants access to the Data. This includes the User, the individuals listed in Appendix II and any other individuals for whom the User Institution subsequently requests access to the Data. Details of the initial Authorized Personnel are set out in Appendix II. Data: The managed access datasets to which the User Institution has requested access. Data Producers: BostonGene Corporation and the collaborators listed in Appendix I responsible for the development, organization, and oversight of this Data. External Collaborator: A collaborator of the User, working for an institution other than the User Institution. Project: The project for which the User Institution has requested access to these Data. A description of the Project is set out in Appendix II. Publications: Includes, without limitation, articles published in print journals, electronic journals, reviews, books, posters and other written and verbal presentations of research. Research Participant: An individual whose data form part of these Data. Research Purposes: Shall mean research that is seeking to advance the understanding of genetics and genomics, including the treatment of disorders, and work on statistical methods that may be applied to such research. User: The principal investigator for the Project. User Institution(s): The Institution that has requested access to the Data. 1. The User Institution agrees to only use these Data for the purpose of the Project (described in Appendix II) and only for Research Purposes. The User Institution further agrees that it will only use these Data for Research Purposes which are within the limitations (if any) set out in Appendix I. The User Institution also agrees that it will submit a report to the BostonGene Data Access Committee, if requested, on completion of the Research Purpose. The BostonGene Data Access Committee agrees to treat the report and all information, data, results, and conclusions contained within such report as confidential information belonging to the User Institution. 2. The User Institution agrees to preserve, at all times, the confidentiality of these Data. In particular, it undertakes not to use, or attempt to use these Data to compromise or otherwise infringe the confidentiality of information on Research Participants. Without prejudice to the generality of the foregoing, the User Institution agrees to use at least the measures set out in Appendix I to protect these Data. The User Institution understands and acknowledges that the Data is protected by copyright and other intellectual property rights, and that duplication, except as reasonably required to carry out research with the Data, or sale of all or part of the Data on any media is not permitted. 3. The User Institution agrees to protect the confidentiality of Research Participants in any research papers or publications that they prepare by taking all reasonable care to limit the possibility of identification. 4. The User Institution agrees not to link or combine these Data to other information or archived data available in a way that could re-identify the Research Participants, even if access to that data has been formally granted to the User Institution or is freely available without restriction. 5. The User Institution agrees only to transfer or disclose these Data, in whole or part, or any material derived from these Data, to the Authorized Personnel. Should the User Institution wish to share these Data with an External Collaborator, the External Collaborator must complete a separate application for access to these Data. 6. The User Institution agrees that the Data Producers, and all other parties involved in the creation, funding or protection of these Data: a) make no warranty or representation, express or implied as to the accuracy, quality or comprehensiveness of these Data; b) exclude to the fullest extent permitted by law all liability for actions, claims, proceedings, demands, losses (including but not limited to loss of profit), costs, awards damages and payments made by the Recipient that may arise (whether directly or indirectly) in any way whatsoever from the Recipient’s use of these Data or from the unavailability of, or break in access to, these Data for whatever reason and; c) bear no responsibility for the accuracy, comprehensiveness, further analysis or interpretation of these Data. 7. The User Institution agrees to follow the Fort Lauderdale Guidelines (http://www.wellcome.ac.uk/stellent/groups/corporatesite/@policy_communications/documents/web_document/wtd003207.pdf ) and the Toronto Statement (http://www.nature.com/nature/journal/v461/n7261/full/461168a.html). This includes but is not limited to recognizing the contribution of the Data Producers and including a proper acknowledgement in all reports or publications resulting from the use of these Data. 8. The User Institution agrees to follow the Publication Policy in Appendix III. This includes respecting the moratorium period for the Data Producers to publish the first peer-reviewed report describing and analyzing these Data. You agree to acknowledge in any such publication that the research makes use of Data generated by BostonGene Corporation. 9. You recognize that nothing in this Agreement shall operate to transfer to the User Institution any intellectual property rights relating to the Data. The User Institution has the right to develop intellectual property based on the comparisons with their own data. Any invention or discovery made by User and/or User Institution arising out of the use of the Data (each a “Data Invention”) shall be promptly disclosed to BostonGene Corporation in writing. User Institution hereby grants to BostonGene Corporation a royalty-free, fully-paid, non-exclusive license (i) to make, use and import such Data Invention for internal research purposes; and (ii) to make, use, offer to sell, sell and import such Data Invention to the extent necessary to commercialize BostonGene Corporation product(s), which license shall be sublicensable to BostonGene Corporation’s affiliates and development and commercialization collaborators on such product(s). 10. The User Institution agrees to destroy/discard the Data held, once it is no longer used for the Project, unless obliged to retain the data for archival purposes in conformity with audit or legal requirements. 11. The User Institution will notify BostonGene Corporation within 30 days of any changes or departures of Authorized Personnel. 12. The User Institution will notify BostonGene Corporation prior to any significant changes to the protocol for the Project. 13. The User Institution will notify BostonGene Corporation as soon as it becomes aware of a breach of the terms or conditions of this agreement. 14. BostonGene Corporation may terminate this agreement for convenience by written notice to the User Institution. If this agreement terminates for any reason, the User Institution will be required to destroy any Data held, including copies and backup copies. This clause does not prevent the User Institution from retaining these data for archival purpose in conformity with audit or legal requirements. 15. The User Institution accepts that it may be necessary for the Data Producers to alter the terms of this agreement from time to time. As an example, this may include specific provisions relating to the Data required by Data Producers other than BostonGene Corporation. In the event that changes are required, the Data Producers or their appointed agent will contact the User Institution to inform it of the changes and the User Institution may elect to accept the changes or terminate the agreement. 16. If requested, the User Institution will allow data security and management documentation to be inspected to verify that it is complying with the terms of this agreement. 17. The User Institution agrees to distribute a copy of these terms to the Authorized Personnel. The User Institution will procure that the Authorized Personnel comply with the terms of this agreement. 18. This agreement (and any dispute, controversy, proceedings or claim of whatever nature arising out of this agreement or its formation) shall be construed, interpreted and governed by the laws of the Commonwealth of Massachusetts, United States. Nothing herein shall create any association, partnership, joint venture, fiduciary duty or the relation of principal and agent between the parties hereto, it being understood that each party is acting as an independent contractor, and neither party shall have the authority to bind the other or the other’s representatives in any way. Agreed for User Institution Signature: Name: Title: Date: Principal Investigator I confirm that I have read and understood this Agreement. Signature: Name: Title: Date: Agreed for BostonGene Corporation Signature: Name: Title: Date: APPENDIX I – DATASET DETAILS APPENDIX II ––PROJECT DETAILS APPENDIX III –– PUBLICATION POLICY APPENDIX I – DATASET DETAILS (to be completed by the data producer before passing to applicant) Dataset reference (EGA Study ID and Dataset Details) Name of project that created the dataset Names of other data producers/collaborators Specific limitations on areas of research Minimum protection measures required File access: Data can be held in unencrypted files on an institutional compute system, with Unix user group read/write access for one or more appropriate groups but not Unix world read/write access behind a secure firewall. Laptops holding these data should have password protected logins and screenlocks (set to lock after 5 min of inactivity). If held on USB keys or other portable hard drives, the data must be encrypted. APPENDIX II – PROJECT DETAILS (to be completed by the Requestor) Details of dataset requested i.e., EGA Study and Dataset Accession Number Brief abstract of the Project in which the Data will be used (500 words max) All Individuals who the User Institution to be named as registered users Name of Registered User Email Job Title Supervisor* All Individuals that should have an account created at the EGA Name of Registered User Email Job Title APPENDIX III – PUBLICATION POLICY BostonGene Corporation intends to publish the results of their analysis of this dataset and do not consider its deposition into public databases to be the equivalent of such publications. BostonGene Corporation anticipates that the dataset could be useful to other qualified researchers for a variety of purposes. However, some areas of work are subject to a publication moratorium. The publication moratorium covers any publications (including oral communications) that describe the use of the dataset. For research papers, submission for publication should not occur until 6 months after these data were first made available on the relevant hosting database, unless BostonGene Corporation has provided written consent to earlier submission. In any publications based on these data, please describe how the data can be accessed, including the name of the hosting database (e.g., The European Genome-phenome Archive at the European Bioinformatics Institute) and its accession numbers (e.g., EGAS00000000029), and acknowledge its use in a form agreed by the User Institution with BostonGene Corporation.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001006272 RNASeq

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
EGAF00006167247 fastq.gz 365.4 MB
EGAF00006167248 fastq.gz 371.7 MB
EGAF00006167249 fastq.gz 3.1 GB
EGAF00006167250 fastq.gz 3.1 GB
EGAF00006167251 fastq.gz 2.8 GB
EGAF00006167252 fastq.gz 2.9 GB
EGAF00006167253 fastq.gz 4.1 GB
EGAF00006167254 fastq.gz 4.2 GB
EGAF00006167255 fastq.gz 2.0 GB
EGAF00006167256 fastq.gz 2.0 GB
EGAF00006167257 fastq.gz 827.4 MB
EGAF00006167258 fastq.gz 788.1 MB
EGAF00006167259 fastq.gz 2.1 GB
EGAF00006167260 fastq.gz 2.1 GB
EGAF00006167261 fastq.gz 2.8 GB
EGAF00006167262 fastq.gz 2.8 GB
EGAF00006167263 fastq.gz 1.6 GB
EGAF00006167264 fastq.gz 1.6 GB
EGAF00006167265 fastq.gz 3.4 GB
EGAF00006167266 fastq.gz 3.6 GB
EGAF00006167267 fastq.gz 3.2 GB
EGAF00006167268 fastq.gz 3.3 GB
EGAF00006167269 fastq.gz 3.3 GB
EGAF00006167270 fastq.gz 3.3 GB
EGAF00006167271 fastq.gz 766.6 MB
EGAF00006167272 fastq.gz 798.9 MB
EGAF00006167273 fastq.gz 959.1 MB
EGAF00006167274 fastq.gz 999.4 MB
EGAF00006167275 fastq.gz 2.8 GB
EGAF00006167276 fastq.gz 2.8 GB
EGAF00006167277 fastq.gz 949.4 MB
EGAF00006167278 fastq.gz 999.1 MB
EGAF00006167279 fastq.gz 858.8 MB
EGAF00006167280 fastq.gz 895.6 MB
EGAF00006167281 fastq.gz 730.8 MB
EGAF00006167282 fastq.gz 758.6 MB
EGAF00006167283 fastq.gz 2.5 GB
EGAF00006167284 fastq.gz 2.6 GB
EGAF00006167285 fastq.gz 761.4 MB
EGAF00006167286 fastq.gz 794.6 MB
EGAF00006167287 fastq.gz 5.1 GB
EGAF00006167288 fastq.gz 5.2 GB
EGAF00006167289 fastq.gz 501.4 MB
EGAF00006167290 fastq.gz 522.8 MB
EGAF00006167291 fastq.gz 3.0 GB
EGAF00006167292 fastq.gz 2.9 GB
EGAF00006167293 fastq.gz 3.6 GB
EGAF00006167294 fastq.gz 3.8 GB
EGAF00006167295 fastq.gz 4.2 GB
EGAF00006167296 fastq.gz 4.3 GB
EGAF00006167297 fastq.gz 2.9 GB
EGAF00006167298 fastq.gz 3.0 GB
EGAF00006167299 fastq.gz 2.8 GB
EGAF00006167300 fastq.gz 2.9 GB
EGAF00006167301 fastq.gz 7.1 GB
EGAF00006167302 fastq.gz 7.4 GB
EGAF00006167303 fastq.gz 5.5 GB
EGAF00006167304 fastq.gz 5.7 GB
EGAF00006167305 fastq.gz 2.0 GB
EGAF00006167306 fastq.gz 2.1 GB
EGAF00006167307 fastq.gz 4.7 GB
EGAF00006167308 fastq.gz 4.9 GB
EGAF00006167309 fastq.gz 4.0 GB
EGAF00006167310 fastq.gz 4.2 GB
EGAF00006167311 fastq.gz 2.0 GB
EGAF00006167312 fastq.gz 2.1 GB
EGAF00006167313 fastq.gz 2.6 GB
EGAF00006167314 fastq.gz 2.6 GB
EGAF00006167315 fastq.gz 2.2 GB
EGAF00006167316 fastq.gz 2.2 GB
EGAF00006167317 fastq.gz 3.4 GB
EGAF00006167318 fastq.gz 3.5 GB
EGAF00006167319 fastq.gz 2.0 GB
EGAF00006167320 fastq.gz 2.1 GB
EGAF00006167321 fastq.gz 2.2 GB
EGAF00006167322 fastq.gz 2.2 GB
EGAF00006167323 fastq.gz 4.0 GB
EGAF00006167324 fastq.gz 4.1 GB
EGAF00006167325 fastq.gz 5.2 GB
EGAF00006167326 fastq.gz 5.4 GB
EGAF00006167327 fastq.gz 4.5 GB
EGAF00006167328 fastq.gz 4.7 GB
EGAF00006167329 fastq.gz 5.4 GB
EGAF00006167330 fastq.gz 5.3 GB
EGAF00006167331 fastq.gz 4.7 GB
EGAF00006167332 fastq.gz 5.1 GB
EGAF00006167333 fastq.gz 5.3 GB
EGAF00006167334 fastq.gz 4.9 GB
EGAF00006167335 fastq.gz 1.9 GB
EGAF00006167336 fastq.gz 2.0 GB
EGAF00006167337 fastq.gz 2.8 GB
EGAF00006167338 fastq.gz 2.6 GB
EGAF00006167339 fastq.gz 4.9 GB
EGAF00006167340 fastq.gz 5.2 GB
EGAF00006167341 fastq.gz 4.1 GB
EGAF00006167342 fastq.gz 4.2 GB
EGAF00006167343 fastq.gz 4.7 GB
EGAF00006167344 fastq.gz 4.8 GB
EGAF00006167345 fastq.gz 5.0 GB
EGAF00006167346 fastq.gz 4.9 GB
EGAF00006167347 fastq.gz 3.1 GB
EGAF00006167348 fastq.gz 3.3 GB
EGAF00006167349 fastq.gz 3.4 GB
EGAF00006167350 fastq.gz 3.5 GB
EGAF00006167351 fastq.gz 4.5 GB
EGAF00006167352 fastq.gz 4.6 GB
EGAF00006167353 fastq.gz 4.5 GB
EGAF00006167354 fastq.gz 4.8 GB
EGAF00006167355 fastq.gz 5.2 GB
EGAF00006167356 fastq.gz 5.4 GB
EGAF00006167357 fastq.gz 3.6 GB
EGAF00006167358 fastq.gz 3.5 GB
EGAF00006167359 fastq.gz 5.0 GB
EGAF00006167360 fastq.gz 5.3 GB
EGAF00006167361 fastq.gz 4.8 GB
EGAF00006167362 fastq.gz 4.9 GB
EGAF00006167363 fastq.gz 3.9 GB
EGAF00006167364 fastq.gz 4.1 GB
EGAF00006167365 fastq.gz 5.0 GB
EGAF00006167366 fastq.gz 5.2 GB
EGAF00006167367 fastq.gz 4.4 GB
EGAF00006167368 fastq.gz 4.5 GB
EGAF00006167369 fastq.gz 3.1 GB
EGAF00006167370 fastq.gz 3.2 GB
EGAF00006167371 fastq.gz 2.0 GB
EGAF00006167372 fastq.gz 2.1 GB
EGAF00006167373 fastq.gz 2.2 GB
EGAF00006167374 fastq.gz 2.3 GB
EGAF00006167375 fastq.gz 5.0 GB
EGAF00006167376 fastq.gz 5.2 GB
EGAF00006167377 fastq.gz 5.7 GB
EGAF00006167378 fastq.gz 5.9 GB
EGAF00006167379 fastq.gz 5.1 GB
EGAF00006167380 fastq.gz 5.3 GB
EGAF00006167381 fastq.gz 5.8 GB
EGAF00006167382 fastq.gz 6.0 GB
EGAF00006167383 fastq.gz 2.7 GB
EGAF00006167384 fastq.gz 2.8 GB
EGAF00006167385 fastq.gz 4.2 GB
EGAF00006167386 fastq.gz 4.3 GB
EGAF00006167387 fastq.gz 2.9 GB
EGAF00006167388 fastq.gz 3.0 GB
EGAF00006167389 fastq.gz 1.8 GB
EGAF00006167390 fastq.gz 1.9 GB
EGAF00006167391 fastq.gz 2.9 GB
EGAF00006167392 fastq.gz 3.0 GB
EGAF00006167393 fastq.gz 3.6 GB
EGAF00006167394 fastq.gz 3.5 GB
EGAF00006167395 fastq.gz 2.8 GB
EGAF00006167396 fastq.gz 2.9 GB
EGAF00006167397 fastq.gz 1.0 GB
EGAF00006167398 fastq.gz 1.0 GB
EGAF00006167399 fastq.gz 3.7 GB
EGAF00006167400 fastq.gz 3.9 GB
EGAF00006167401 fastq.gz 3.8 GB
EGAF00006167402 fastq.gz 3.9 GB
EGAF00006167403 fastq.gz 5.7 GB
EGAF00006167404 fastq.gz 5.8 GB
EGAF00006167405 fastq.gz 4.9 GB
EGAF00006167406 fastq.gz 5.1 GB
EGAF00006167407 fastq.gz 5.6 GB
EGAF00006167408 fastq.gz 6.3 GB
EGAF00006167409 fastq.gz 3.6 GB
EGAF00006167410 fastq.gz 3.6 GB
EGAF00006167411 fastq.gz 3.2 GB
EGAF00006167412 fastq.gz 3.0 GB
EGAF00006167413 fastq.gz 1.8 GB
EGAF00006167414 fastq.gz 1.7 GB
EGAF00006167415 fastq.gz 4.5 GB
EGAF00006167416 fastq.gz 4.7 GB
EGAF00006167417 fastq.gz 3.5 GB
EGAF00006167418 fastq.gz 3.5 GB
EGAF00006167419 fastq.gz 3.8 GB
EGAF00006167420 fastq.gz 3.9 GB
EGAF00006167421 fastq.gz 2.0 GB
EGAF00006167422 fastq.gz 2.1 GB
EGAF00006167423 fastq.gz 327.4 MB
EGAF00006167424 fastq.gz 335.3 MB
EGAF00006167425 fastq.gz 2.7 GB
EGAF00006167426 fastq.gz 3.0 GB
EGAF00006167427 fastq.gz 2.6 GB
EGAF00006167428 fastq.gz 2.7 GB
EGAF00006167429 fastq.gz 3.2 GB
EGAF00006167430 fastq.gz 3.2 GB
EGAF00006167431 fastq.gz 3.2 GB
EGAF00006167432 fastq.gz 3.4 GB
EGAF00006167433 fastq.gz 3.6 GB
EGAF00006167434 fastq.gz 3.7 GB
EGAF00006167435 fastq.gz 3.3 GB
EGAF00006167436 fastq.gz 3.4 GB
EGAF00006167437 fastq.gz 3.4 GB
EGAF00006167438 fastq.gz 3.4 GB
EGAF00006167439 fastq.gz 4.5 GB
EGAF00006167440 fastq.gz 4.7 GB
EGAF00006167441 fastq.gz 3.7 GB
EGAF00006167442 fastq.gz 3.7 GB
EGAF00006167443 fastq.gz 3.1 GB
EGAF00006167444 fastq.gz 3.2 GB
EGAF00006167445 fastq.gz 2.8 GB
EGAF00006167446 fastq.gz 2.9 GB
EGAF00006167447 fastq.gz 3.7 GB
EGAF00006167448 fastq.gz 3.9 GB
EGAF00006167449 fastq.gz 3.0 GB
EGAF00006167450 fastq.gz 3.1 GB
EGAF00006167451 fastq.gz 3.0 GB
EGAF00006167452 fastq.gz 3.1 GB
EGAF00006167453 fastq.gz 2.9 GB
EGAF00006167454 fastq.gz 2.8 GB
EGAF00006167455 fastq.gz 4.0 GB
EGAF00006167456 fastq.gz 4.2 GB
EGAF00006167457 fastq.gz 6.3 GB
EGAF00006167458 fastq.gz 6.4 GB
EGAF00006167459 fastq.gz 1.8 GB
EGAF00006167460 fastq.gz 1.9 GB
EGAF00006167461 fastq.gz 3.4 GB
EGAF00006167462 fastq.gz 3.6 GB
EGAF00006167463 fastq.gz 2.0 GB
EGAF00006167464 fastq.gz 2.0 GB
EGAF00006167465 fastq.gz 2.3 GB
EGAF00006167466 fastq.gz 2.3 GB
EGAF00006167467 fastq.gz 2.1 GB
EGAF00006167468 fastq.gz 2.2 GB
EGAF00006167469 fastq.gz 9.3 GB
EGAF00006167470 fastq.gz 9.7 GB
EGAF00006167471 fastq.gz 3.4 GB
EGAF00006167472 fastq.gz 3.4 GB
EGAF00006167473 fastq.gz 3.3 GB
EGAF00006167474 fastq.gz 3.4 GB
EGAF00006167475 fastq.gz 4.2 GB
EGAF00006167476 fastq.gz 4.3 GB
EGAF00006167477 fastq.gz 3.4 GB
EGAF00006167478 fastq.gz 3.6 GB
EGAF00006167479 fastq.gz 3.4 GB
EGAF00006167480 fastq.gz 3.5 GB
EGAF00006167481 fastq.gz 3.3 GB
EGAF00006167482 fastq.gz 3.3 GB
EGAF00006167483 fastq.gz 2.7 GB
EGAF00006167484 fastq.gz 2.9 GB
EGAF00006167485 fastq.gz 2.6 GB
EGAF00006167486 fastq.gz 2.8 GB
EGAF00006167487 fastq.gz 3.5 GB
EGAF00006167488 fastq.gz 3.6 GB
EGAF00006167489 fastq.gz 3.7 GB
EGAF00006167490 fastq.gz 3.4 GB
EGAF00006167491 fastq.gz 3.0 GB
EGAF00006167492 fastq.gz 3.1 GB
EGAF00006167493 fastq.gz 4.5 GB
EGAF00006167494 fastq.gz 4.5 GB
EGAF00006167495 fastq.gz 2.6 GB
EGAF00006167496 fastq.gz 2.6 GB
EGAF00006167497 fastq.gz 4.7 GB
EGAF00006167498 fastq.gz 4.8 GB
EGAF00006167499 fastq.gz 4.1 GB
EGAF00006167500 fastq.gz 4.1 GB
EGAF00006167501 fastq.gz 4.1 GB
EGAF00006167502 fastq.gz 4.2 GB
EGAF00006167503 fastq.gz 3.9 GB
EGAF00006167504 fastq.gz 3.8 GB
EGAF00006167505 fastq.gz 5.3 GB
EGAF00006167506 fastq.gz 5.6 GB
EGAF00006167507 fastq.gz 2.8 GB
EGAF00006167508 fastq.gz 2.9 GB
EGAF00006167509 fastq.gz 2.9 GB
EGAF00006167510 fastq.gz 2.9 GB
EGAF00006167511 fastq.gz 2.1 GB
EGAF00006167512 fastq.gz 2.2 GB
EGAF00006167513 fastq.gz 3.9 GB
EGAF00006167514 fastq.gz 4.1 GB
EGAF00006167515 fastq.gz 3.3 GB
EGAF00006167516 fastq.gz 3.4 GB
EGAF00006167517 fastq.gz 3.1 GB
EGAF00006167518 fastq.gz 3.2 GB
EGAF00006167519 fastq.gz 3.8 GB
EGAF00006167520 fastq.gz 3.8 GB
EGAF00006167521 fastq.gz 3.0 GB
EGAF00006167522 fastq.gz 3.1 GB
EGAF00006167523 fastq.gz 3.2 GB
EGAF00006167524 fastq.gz 3.4 GB
EGAF00006167525 fastq.gz 2.3 GB
EGAF00006167526 fastq.gz 2.4 GB
EGAF00006167527 fastq.gz 735.9 MB
EGAF00006167528 fastq.gz 739.8 MB
EGAF00006167529 fastq.gz 3.4 GB
EGAF00006167530 fastq.gz 3.4 GB
EGAF00006167531 fastq.gz 4.1 GB
EGAF00006167532 fastq.gz 3.4 GB
EGAF00006167533 fastq.gz 2.8 GB
EGAF00006167534 fastq.gz 2.9 GB
EGAF00006167535 fastq.gz 2.8 GB
EGAF00006167536 fastq.gz 3.0 GB
EGAF00006167537 fastq.gz 4.7 GB
EGAF00006167538 fastq.gz 4.8 GB
EGAF00006167539 fastq.gz 3.6 GB
EGAF00006167540 fastq.gz 3.6 GB
EGAF00006167541 fastq.gz 2.9 GB
EGAF00006167542 fastq.gz 3.0 GB
EGAF00006167543 fastq.gz 3.0 GB
EGAF00006167544 fastq.gz 2.9 GB
EGAF00006167545 fastq.gz 4.0 GB
EGAF00006167546 fastq.gz 4.1 GB
EGAF00006167547 fastq.gz 631.5 MB
EGAF00006167548 fastq.gz 623.9 MB
EGAF00006167549 fastq.gz 5.4 GB
EGAF00006167550 fastq.gz 5.5 GB
EGAF00006167551 fastq.gz 3.5 GB
EGAF00006167552 fastq.gz 3.7 GB
EGAF00006167553 fastq.gz 3.9 GB
EGAF00006167554 fastq.gz 3.6 GB
EGAF00006167555 fastq.gz 3.5 GB
EGAF00006167556 fastq.gz 3.6 GB
EGAF00006167557 fastq.gz 4.3 GB
EGAF00006167558 fastq.gz 4.1 GB
EGAF00006167559 fastq.gz 3.3 GB
EGAF00006167560 fastq.gz 3.0 GB
EGAF00006167561 fastq.gz 3.2 GB
EGAF00006167562 fastq.gz 3.2 GB
EGAF00006167563 fastq.gz 4.0 GB
EGAF00006167564 fastq.gz 4.1 GB
EGAF00006167565 fastq.gz 3.6 GB
EGAF00006167566 fastq.gz 3.7 GB
EGAF00006167567 fastq.gz 4.2 GB
EGAF00006167568 fastq.gz 4.3 GB
EGAF00006167569 fastq.gz 5.5 GB
EGAF00006167570 fastq.gz 5.6 GB
EGAF00006167571 fastq.gz 2.8 GB
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EGAF00006167821 fastq.gz 2.8 GB
EGAF00006167822 fastq.gz 2.8 GB
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EGAF00006167826 fastq.gz 6.3 GB
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EGAF00006167829 fastq.gz 3.5 GB
EGAF00006167830 fastq.gz 3.5 GB
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EGAF00006167858 fastq.gz 778.3 MB
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EGAF00006167865 fastq.gz 2.5 GB
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EGAF00006167869 fastq.gz 732.9 MB
EGAF00006167870 fastq.gz 727.7 MB
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EGAF00006167872 fastq.gz 3.3 GB
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EGAF00006167874 fastq.gz 3.1 GB
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EGAF00006167876 fastq.gz 808.4 MB
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EGAF00006167879 fastq.gz 2.1 GB
EGAF00006167880 fastq.gz 2.2 GB
EGAF00006167881 fastq.gz 910.6 MB
EGAF00006167882 fastq.gz 949.4 MB
EGAF00006167883 fastq.gz 3.7 GB
EGAF00006167884 fastq.gz 3.7 GB
EGAF00006167885 fastq.gz 706.8 MB
EGAF00006167886 fastq.gz 729.0 MB
EGAF00006167887 fastq.gz 2.0 GB
EGAF00006167888 fastq.gz 2.0 GB
EGAF00006167889 fastq.gz 3.8 GB
EGAF00006167890 fastq.gz 4.1 GB
EGAF00006167891 fastq.gz 3.7 GB
EGAF00006167892 fastq.gz 3.8 GB
EGAF00006167893 fastq.gz 3.4 GB
EGAF00006167894 fastq.gz 3.7 GB
EGAF00006167895 fastq.gz 3.4 GB
EGAF00006167896 fastq.gz 3.5 GB
EGAF00006167897 fastq.gz 2.7 GB
EGAF00006167898 fastq.gz 2.7 GB
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EGAF00006167900 fastq.gz 1.4 GB
EGAF00006167901 fastq.gz 3.0 GB
EGAF00006167902 fastq.gz 3.0 GB
EGAF00006167903 fastq.gz 3.5 GB
EGAF00006167904 fastq.gz 3.6 GB
EGAF00006167905 fastq.gz 2.5 GB
EGAF00006167906 fastq.gz 2.7 GB
EGAF00006167907 fastq.gz 4.5 GB
EGAF00006167908 fastq.gz 4.5 GB
EGAF00006167909 fastq.gz 3.9 GB
EGAF00006167910 fastq.gz 3.9 GB
EGAF00006167911 fastq.gz 1.9 GB
EGAF00006167912 fastq.gz 1.9 GB
EGAF00006167913 fastq.gz 2.9 GB
EGAF00006167914 fastq.gz 3.0 GB
EGAF00006167915 fastq.gz 1.9 GB
EGAF00006167916 fastq.gz 2.1 GB
EGAF00006167917 fastq.gz 3.3 GB
EGAF00006167918 fastq.gz 3.4 GB
EGAF00006167919 fastq.gz 3.7 GB
EGAF00006167920 fastq.gz 3.7 GB
EGAF00006167921 fastq.gz 1.7 GB
EGAF00006167922 fastq.gz 1.8 GB
EGAF00006167923 fastq.gz 3.0 GB
EGAF00006167924 fastq.gz 3.1 GB
EGAF00006167925 fastq.gz 3.0 GB
EGAF00006167926 fastq.gz 3.1 GB
EGAF00006167927 fastq.gz 3.5 GB
EGAF00006167928 fastq.gz 3.6 GB
EGAF00006167929 fastq.gz 2.9 GB
EGAF00006167930 fastq.gz 3.1 GB
EGAF00006167931 fastq.gz 3.5 GB
EGAF00006167932 fastq.gz 3.7 GB
EGAF00006167933 fastq.gz 5.8 GB
EGAF00006167934 fastq.gz 5.8 GB
EGAF00006167935 fastq.gz 5.9 GB
EGAF00006167936 fastq.gz 5.6 GB
EGAF00006167937 fastq.gz 722.4 MB
EGAF00006167938 fastq.gz 753.0 MB
EGAF00006167939 fastq.gz 2.4 GB
EGAF00006167940 fastq.gz 2.6 GB
EGAF00006167941 fastq.gz 804.1 MB
EGAF00006167942 fastq.gz 849.8 MB
696 Files (2.3 TB)