About file location

As the EGA is co-managed by the EMBL-EBI and CRG, files could be located in the UK and/or Spain, as indicated by their respective flags.

Alternatively, files could be located in one of the international Federated EGA nodes, as indicated next to the node's flag.
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RNA-sequencing of ex vivo exhausted human antigen-specific T cells

Tumor-specific T cells are frequently exhausted by chronic antigenic stimulation. To explore new pathways for reinvigoration of anti-tumor immune functions, we developed a human ex vivo exhaustion model by repetitive antigenic stimulation of primary CD8 T cells. This results in T cells that resemble patient-derived T cells in tumors on a phenotypic and transcriptional level. Four human healhy donor CD8+ T cells were isolated, transduced with an NY-ESO-1 TCR lentivirus construct, stimulated in four different conditions (Trested, Ttumor, Tex, Teff) with T2 tumor cells and specific peptides over 12 days. Cells were then sorted for TCR Vbeta 13.1+ (NY-ESO-1 TCR) CD8+ CD3+ CD56- CD4- DAPI- cells. RNA-seq TruSeq libraries were generated from polyA-enriched mRNA isolated from the samples, and sequenced in paired-end mode (2x51bp) on 2 lanes of an Illumina NovaSeq 6000 flow-cell. FASTQ sequence files were generated with the Illumina RTA version 3.4.4 and Base-calling Version bcl2fastq-2.20.0.422.

Request Access

DUO:0000006
version: 2021-02-23

health or medical or biomedical research

This data use permission indicates that use is allowed for health/medical/biomedical purposes; does not include the study of population origins or ancestry.

DUO:0000024
version: 2021-02-23

publication moratorium

This data use modifier indicates that requestor agrees not to publish results of studies until a specific date.

not available - do not use

not available - do not use

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001006794 Other
  • Set back the correct policy number.
  • Updated policy to match request of MF-DAC.
  • Updated a data type to RNASeq
  • Dataset Released

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
i
EGAF00007774187 fastq.gz 655.3 MB Report
EGAF00007774188 fastq.gz 690.6 MB Report
EGAF00007774189 fastq.gz 648.1 MB Report
EGAF00007774190 fastq.gz 682.7 MB Report
EGAF00007774191 fastq.gz 638.1 MB Report
EGAF00007774192 fastq.gz 664.5 MB Report
EGAF00007774193 fastq.gz 629.9 MB Report
EGAF00007774194 fastq.gz 655.8 MB Report
EGAF00007774195 fastq.gz 875.5 MB Report
EGAF00007774196 fastq.gz 914.6 MB Report
EGAF00007774197 fastq.gz 864.6 MB Report
EGAF00007774198 fastq.gz 902.5 MB Report
EGAF00007774199 fastq.gz 771.2 MB Report
EGAF00007774200 fastq.gz 805.2 MB Report
EGAF00007774201 fastq.gz 760.9 MB Report
EGAF00007774202 fastq.gz 794.1 MB Report
EGAF00007774203 fastq.gz 817.0 MB Report
EGAF00007774204 fastq.gz 852.2 MB Report
EGAF00007774205 fastq.gz 808.1 MB Report
EGAF00007774206 fastq.gz 842.5 MB Report
EGAF00007774207 fastq.gz 788.0 MB Report
EGAF00007774208 fastq.gz 818.2 MB Report
EGAF00007774209 fastq.gz 777.9 MB Report
EGAF00007774210 fastq.gz 807.5 MB Report
EGAF00007774211 fastq.gz 761.8 MB Report
EGAF00007774212 fastq.gz 801.2 MB Report
EGAF00007774213 fastq.gz 753.5 MB Report
EGAF00007774214 fastq.gz 792.1 MB Report
EGAF00007774215 fastq.gz 779.6 MB Report
EGAF00007774216 fastq.gz 811.4 MB Report
EGAF00007774217 fastq.gz 769.3 MB Report
EGAF00007774218 fastq.gz 800.3 MB Report
EGAF00007774219 fastq.gz 662.1 MB Report
EGAF00007774220 fastq.gz 704.0 MB Report
EGAF00007774221 fastq.gz 655.5 MB Report
EGAF00007774222 fastq.gz 696.5 MB Report
EGAF00007774223 fastq.gz 675.8 MB Report
EGAF00007774224 fastq.gz 706.5 MB Report
EGAF00007774225 fastq.gz 667.2 MB Report
EGAF00007774226 fastq.gz 697.2 MB Report
EGAF00007774227 fastq.gz 603.5 MB Report
EGAF00007774228 fastq.gz 634.7 MB Report
EGAF00007774229 fastq.gz 596.2 MB Report
EGAF00007774230 fastq.gz 626.6 MB Report
EGAF00007774231 fastq.gz 606.0 MB Report
EGAF00007774232 fastq.gz 637.9 MB Report
EGAF00007774233 fastq.gz 598.1 MB Report
EGAF00007774234 fastq.gz 629.1 MB Report
EGAF00007774235 fastq.gz 681.3 MB Report
EGAF00007774236 fastq.gz 713.8 MB Report
EGAF00007774237 fastq.gz 671.7 MB Report
EGAF00007774238 fastq.gz 703.4 MB Report
EGAF00007774239 fastq.gz 769.1 MB Report
EGAF00007774240 fastq.gz 802.4 MB Report
EGAF00007774241 fastq.gz 759.7 MB Report
EGAF00007774242 fastq.gz 792.1 MB Report
EGAF00007774243 fastq.gz 793.0 MB Report
EGAF00007774244 fastq.gz 830.2 MB Report
EGAF00007774245 fastq.gz 785.3 MB Report
EGAF00007774246 fastq.gz 821.8 MB Report
EGAF00007774247 fastq.gz 699.0 MB Report
EGAF00007774248 fastq.gz 724.5 MB Report
EGAF00007774249 fastq.gz 689.6 MB Report
EGAF00007774250 fastq.gz 714.6 MB Report
64 Files (47.1 GB)