shallow WGS of cell free DNA
12.5 ng of cfDNA was used as input for shallow whole-genome sequencing (sWGS), aiming for a coverage of x0.2-0.4-fold. Library preparation was performed using the TruSeq Nano DNA High Throughput Library Prep Kit (Illumina, San Diego, CA, USA) on an automated Hamilton STAR liquid handling system (Hamilton, Germany GmbH, Robotics, Gräfeling, Germany) with dual indexing, and sequencing was performed on the NextSeq500/550 platform (Illumina). The fraction of tumor-derived DNA in cell-free DNA was estimated using the R package ichorCNA.
- 1 sample
- DAC: EGAC00001003001
- Technology: NextSeq 500
Data access policy for NPY methylated ctDNA is a promising biomarker for treatment response monitoring in metastatic colorectal cancer
Data access will be granted by the data access committee after approval of the project proposal submitted by the researcher(s). The DAC will evaluate access by assessment of conflict(s) of interest, but foremost, whether the submitted proposal is in line with the consent provided by the patients prior to the study in which this data was generated.
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| EGAS00001006820 | Cancer Genomics |