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Clonal dynamics after allogeneic haematopoietic cell transplantation using genome-wide somatic mutations - WGS

Allogeneic haematopoietic cell transplantation (HCT) replaces the stem cells responsible for blood production with those harvested from a donor, and is received by 40,000 patients worldwide each year. To quantify dynamics of long-term stem cell engraftment, we sequenced whole genomes of 2,824 single-cell-derived haematopoietic colonies from blood samples of 10 donor-recipient pairs taken 9-31 years after HLA-matched sibling HCT. With younger donors, 10,000-50,000 stem cells had engrafted and were still contributing to haematopoiesis at time of sampling, but estimates were 10-fold lower with older donors. Engrafted stem cells made multilineage contributions to myeloid, B-lymphoid and T-lymphoid populations, although individual clones often showed biases towards one or other mature cell type. Recipients had lower clonal diversity than matched donors, equivalent to ~10-15 years of additional ageing, arising from up to 25-fold greater expansion of stem cell clones. An HCT-related population bottleneck alone could not explain these differences: instead, phylogenetic trees evinced two distinct modes of HCT-specific selection. In 'pruning selection', cell divisions underpinning recipient-enriched clonal expansions had occurred in the donor, preceding transplant - their selective advantage derived from preferential mobilisation, harvest, survival ex vivo or initial homing. In 'growth selection', cell divisions underpinning clonal expansion occurred through proliferative advantage in the recipient's marrow after homing - clones with multiple driver mutations especially demonstrated this pattern. Uprooting stem cells from their native environment and transplanting them to foreign soil exaggerates selective pressures, distorting and accelerating the loss of clonal diversity compared to the unperturbed haematopoiesis of donors.

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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001003744 Whole Genome Sequencing

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00008067617 cram 10.9 GB
EGAF00008067618 cram 13.3 GB
EGAF00008067619 cram 12.2 GB
EGAF00008067620 cram 11.8 GB
EGAF00008067621 cram 12.9 GB
EGAF00008067622 cram 12.4 GB
EGAF00008067623 cram 12.0 GB
EGAF00008067624 cram 12.8 GB
EGAF00008067625 cram 13.2 GB
EGAF00008067626 cram 12.8 GB
EGAF00008067627 cram 9.9 GB
EGAF00008067628 cram 12.8 GB
EGAF00008067629 cram 11.0 GB
EGAF00008067630 cram 10.3 GB
EGAF00008067631 cram 14.1 GB
EGAF00008067632 cram 12.3 GB
EGAF00008067633 cram 11.1 GB
EGAF00008067634 cram 13.1 GB
EGAF00008067635 cram 12.7 GB
EGAF00008067636 cram 9.5 GB
EGAF00008067637 cram 12.0 GB
EGAF00008067638 cram 13.7 GB
EGAF00008067639 cram 12.1 GB
EGAF00008067640 cram 11.6 GB
EGAF00008067641 cram 13.5 GB
EGAF00008067642 cram 12.3 GB
EGAF00008067643 cram 10.9 GB
EGAF00008067644 cram 16.6 GB
EGAF00008067645 cram 14.7 GB
EGAF00008067646 cram 15.4 GB
EGAF00008067647 cram 15.4 GB
EGAF00008067648 cram 12.5 GB
EGAF00008067649 cram 11.6 GB
EGAF00008067650 cram 11.1 GB
EGAF00008067651 cram 11.2 GB
EGAF00008067652 cram 10.9 GB
EGAF00008067653 cram 11.9 GB
EGAF00008067654 cram 11.9 GB
EGAF00008067655 cram 11.6 GB
EGAF00008067656 cram 8.1 GB
EGAF00008067657 cram 10.6 GB
EGAF00008067658 cram 9.0 GB
EGAF00008067659 cram 11.5 GB
EGAF00008067660 cram 10.0 GB
EGAF00008067661 cram 10.7 GB
EGAF00008067662 cram 11.1 GB
EGAF00008067663 cram 11.6 GB
EGAF00008067664 cram 13.0 GB
EGAF00008067665 cram 12.6 GB
EGAF00008067666 cram 10.5 GB
EGAF00008067667 cram 10.7 GB
EGAF00008067668 cram 11.4 GB
EGAF00008067669 cram 10.1 GB
EGAF00008067670 cram 14.5 GB
EGAF00008067671 cram 8.7 GB
EGAF00008067672 cram 12.9 GB
EGAF00008067673 cram 7.4 GB
EGAF00008067674 cram 12.7 GB
EGAF00008067675 cram 18.0 GB
EGAF00008067676 cram 14.7 GB
EGAF00008067677 cram 14.5 GB
EGAF00008067678 cram 12.6 GB
EGAF00008067679 cram 14.4 GB
EGAF00008067680 cram 14.0 GB
EGAF00008067681 cram 11.6 GB
EGAF00008067682 cram 10.6 GB
EGAF00008067683 cram 11.1 GB
EGAF00008067684 cram 12.4 GB
EGAF00008067685 cram 10.9 GB
EGAF00008067686 cram 12.8 GB
EGAF00008067687 cram 11.1 GB
EGAF00008067688 cram 12.5 GB
EGAF00008067689 cram 14.0 GB
EGAF00008067690 cram 13.7 GB
EGAF00008067691 cram 12.2 GB
EGAF00008067692 cram 11.0 GB
EGAF00008067693 cram 11.5 GB
EGAF00008067694 cram 12.9 GB
EGAF00008067695 cram 12.9 GB
EGAF00008067696 cram 12.8 GB
EGAF00008067697 cram 13.5 GB
EGAF00008067698 cram 11.1 GB
EGAF00008067699 cram 12.0 GB
EGAF00008067700 cram 9.5 GB
EGAF00008067701 cram 10.2 GB
EGAF00008067702 cram 10.8 GB
EGAF00008067703 cram 10.7 GB
EGAF00008067704 cram 9.4 GB
EGAF00008067705 cram 8.6 GB
EGAF00008067706 cram 9.9 GB
EGAF00008067707 cram 10.6 GB
EGAF00008067708 cram 11.1 GB
EGAF00008067709 cram 10.7 GB
EGAF00008067710 cram 12.0 GB
EGAF00008067711 cram 11.9 GB
EGAF00008067712 cram 11.1 GB
EGAF00008067713 cram 12.3 GB
EGAF00008067714 cram 10.4 GB
EGAF00008067715 cram 9.5 GB
EGAF00008067716 cram 9.4 GB
EGAF00008067717 cram 6.9 GB
EGAF00008067718 cram 7.8 GB
EGAF00008067719 cram 8.0 GB
EGAF00008067720 cram 11.0 GB
EGAF00008067721 cram 7.9 GB
EGAF00008067722 cram 9.7 GB
EGAF00008067723 cram 8.3 GB
EGAF00008067724 cram 8.6 GB
EGAF00008067725 cram 12.4 GB
EGAF00008067726 cram 7.0 GB
EGAF00008067727 cram 13.8 GB
EGAF00008067728 cram 14.1 GB
EGAF00008067729 cram 9.3 GB
EGAF00008067730 cram 16.4 GB
EGAF00008067731 cram 13.7 GB
EGAF00008067732 cram 12.3 GB
EGAF00008067733 cram 11.6 GB
EGAF00008067734 cram 10.8 GB
EGAF00008067735 cram 13.9 GB
EGAF00008067736 cram 13.4 GB
EGAF00008067737 cram 10.9 GB
EGAF00008067738 cram 12.3 GB
EGAF00008067739 cram 13.1 GB
EGAF00008067740 cram 11.3 GB
EGAF00008067741 cram 10.6 GB
EGAF00008067742 cram 14.3 GB
EGAF00008067743 cram 10.1 GB
EGAF00008067744 cram 12.0 GB
EGAF00008067745 cram 10.8 GB
EGAF00008067746 cram 10.2 GB
EGAF00008067747 cram 14.4 GB
EGAF00008067748 cram 9.4 GB
EGAF00008067749 cram 10.8 GB
EGAF00008067750 cram 9.4 GB
EGAF00008067751 cram 8.9 GB
EGAF00008067752 cram 11.7 GB
EGAF00008067753 cram 9.6 GB
EGAF00008067754 cram 13.0 GB
EGAF00008067755 cram 12.3 GB
EGAF00008067756 cram 9.7 GB
EGAF00008067757 cram 11.9 GB
EGAF00008067758 cram 10.8 GB
EGAF00008067759 cram 12.8 GB
EGAF00008067760 cram 10.9 GB
EGAF00008067761 cram 11.8 GB
EGAF00008067762 cram 13.3 GB
EGAF00008067763 cram 11.5 GB
EGAF00008067764 cram 11.5 GB
EGAF00008067765 cram 12.0 GB
EGAF00008067766 cram 12.1 GB
EGAF00008067767 cram 9.9 GB
EGAF00008067768 cram 10.9 GB
EGAF00008067769 cram 7.3 GB
EGAF00008067770 cram 10.2 GB
EGAF00008067771 cram 9.3 GB
EGAF00008067772 cram 9.0 GB
EGAF00008067773 cram 8.5 GB
EGAF00008067774 cram 8.1 GB
EGAF00008067775 cram 9.5 GB
EGAF00008067776 cram 8.7 GB
EGAF00008067777 cram 8.7 GB
EGAF00008067778 cram 9.0 GB
EGAF00008067779 cram 10.9 GB
EGAF00008067780 cram 9.8 GB
EGAF00008067781 cram 10.4 GB
EGAF00008067782 cram 8.7 GB
EGAF00008067783 cram 9.4 GB
EGAF00008067784 cram 7.6 GB
EGAF00008067785 cram 9.3 GB
EGAF00008067786 cram 12.5 GB
EGAF00008067787 cram 10.3 GB
EGAF00008067788 cram 10.7 GB
EGAF00008067789 cram 11.0 GB
EGAF00008067790 cram 8.2 GB
EGAF00008067791 cram 11.9 GB
EGAF00008067792 cram 10.4 GB
EGAF00008067793 cram 9.0 GB
EGAF00008067794 cram 11.8 GB
EGAF00008067795 cram 8.5 GB
EGAF00008067796 cram 10.2 GB
EGAF00008067797 cram 9.5 GB
EGAF00008067798 cram 11.1 GB
EGAF00008067799 cram 11.3 GB
EGAF00008067800 cram 5.5 GB
EGAF00008067801 cram 10.0 GB
EGAF00008067802 cram 8.9 GB
EGAF00008067803 cram 9.0 GB
EGAF00008067804 cram 11.3 GB
EGAF00008067805 cram 8.6 GB
EGAF00008067806 cram 9.7 GB
EGAF00008067807 cram 14.0 GB
EGAF00008067808 cram 11.7 GB
EGAF00008067809 cram 8.6 GB
EGAF00008067810 cram 10.3 GB
EGAF00008067811 cram 9.4 GB
EGAF00008067812 cram 8.9 GB
EGAF00008067813 cram 9.7 GB
EGAF00008067814 cram 6.2 GB
EGAF00008067815 cram 12.7 GB
EGAF00008067816 cram 10.7 GB
EGAF00008067817 cram 9.5 GB
EGAF00008067818 cram 11.4 GB
EGAF00008067819 cram 11.5 GB
EGAF00008067820 cram 8.5 GB
EGAF00008067821 cram 10.5 GB
EGAF00008067822 cram 7.7 GB
EGAF00008067823 cram 8.0 GB
EGAF00008067824 cram 12.1 GB
EGAF00008067825 cram 10.3 GB
EGAF00008067826 cram 9.5 GB
EGAF00008067827 cram 13.0 GB
EGAF00008067828 cram 10.6 GB
EGAF00008067829 cram 12.6 GB
EGAF00008067830 cram 10.8 GB
EGAF00008067831 cram 12.4 GB
EGAF00008067832 cram 7.6 GB
EGAF00008067833 cram 8.7 GB
EGAF00008067834 cram 10.4 GB
EGAF00008067835 cram 11.1 GB
EGAF00008067836 cram 8.1 GB
EGAF00008067837 cram 11.0 GB
EGAF00008067838 cram 7.9 GB
EGAF00008067839 cram 8.8 GB
EGAF00008067840 cram 11.7 GB
EGAF00008067841 cram 9.6 GB
EGAF00008067842 cram 9.9 GB
EGAF00008067843 cram 10.3 GB
EGAF00008067844 cram 9.4 GB
EGAF00008067845 cram 9.9 GB
EGAF00008067846 cram 10.4 GB
EGAF00008067847 cram 9.2 GB
EGAF00008067848 cram 10.1 GB
EGAF00008067849 cram 10.5 GB
EGAF00008067850 cram 11.1 GB
EGAF00008067851 cram 9.4 GB
EGAF00008067852 cram 10.8 GB
EGAF00008067853 cram 11.4 GB
EGAF00008067854 cram 11.0 GB
EGAF00008067855 cram 10.7 GB
EGAF00008067856 cram 10.7 GB
EGAF00008067857 cram 8.4 GB
EGAF00008067858 cram 8.4 GB
EGAF00008067859 cram 11.1 GB
EGAF00008067860 cram 8.8 GB
EGAF00008067861 cram 10.1 GB
EGAF00008067862 cram 10.0 GB
EGAF00008067863 cram 9.4 GB
EGAF00008067864 cram 9.8 GB
EGAF00008067865 cram 9.6 GB
EGAF00008067866 cram 9.4 GB
EGAF00008067867 cram 11.4 GB
EGAF00008067868 cram 9.8 GB
EGAF00008067869 cram 11.1 GB
EGAF00008067870 cram 10.4 GB
EGAF00008067871 cram 10.3 GB
EGAF00008067872 cram 12.5 GB
EGAF00008067873 cram 9.6 GB
EGAF00008067874 cram 9.6 GB
EGAF00008067875 cram 8.0 GB
EGAF00008067876 cram 6.6 GB
EGAF00008067877 cram 9.3 GB
EGAF00008067878 cram 8.7 GB
EGAF00008067879 cram 9.1 GB
EGAF00008067880 cram 7.9 GB
EGAF00008067881 cram 7.1 GB
EGAF00008067882 cram 9.0 GB
EGAF00008067883 cram 9.0 GB
EGAF00008067884 cram 7.5 GB
EGAF00008067885 cram 7.6 GB
EGAF00008067886 cram 12.2 GB
EGAF00008067887 cram 8.1 GB
EGAF00008067888 cram 7.9 GB
EGAF00008067889 cram 8.3 GB
EGAF00008067890 cram 9.8 GB
EGAF00008067891 cram 8.7 GB
EGAF00008067892 cram 8.7 GB
EGAF00008067893 cram 10.2 GB
EGAF00008067894 cram 10.9 GB
EGAF00008067895 cram 11.8 GB
EGAF00008067896 cram 9.5 GB
EGAF00008067897 cram 10.1 GB
EGAF00008067898 cram 10.3 GB
EGAF00008067899 cram 10.2 GB
EGAF00008067900 cram 14.0 GB
EGAF00008067901 cram 10.6 GB
EGAF00008067902 cram 11.0 GB
EGAF00008067903 cram 10.1 GB
EGAF00008067904 cram 12.3 GB
EGAF00008067905 cram 9.5 GB
EGAF00008067906 cram 9.5 GB
EGAF00008067907 cram 11.3 GB
EGAF00008067908 cram 10.1 GB
EGAF00008067909 cram 10.3 GB
EGAF00008067910 cram 12.3 GB
EGAF00008067911 cram 7.1 GB
EGAF00008067912 cram 8.6 GB
EGAF00008067913 cram 11.1 GB
EGAF00008067914 cram 9.5 GB
EGAF00008067915 cram 10.1 GB
EGAF00008067916 cram 7.4 GB
EGAF00008067917 cram 8.3 GB
EGAF00008067918 cram 7.8 GB
EGAF00008067919 cram 9.4 GB
EGAF00008067920 cram 7.3 GB
EGAF00008067921 cram 8.3 GB
EGAF00008067922 cram 7.2 GB
EGAF00008067923 cram 8.6 GB
EGAF00008067924 cram 9.0 GB
EGAF00008067925 cram 9.7 GB
EGAF00008067926 cram 8.2 GB
EGAF00008067927 cram 9.4 GB
EGAF00008067928 cram 8.9 GB
EGAF00008067929 cram 9.8 GB
EGAF00008067930 cram 9.2 GB
EGAF00008067931 cram 7.1 GB
EGAF00008067932 cram 11.0 GB
EGAF00008067933 cram 6.4 GB
EGAF00008067934 cram 8.2 GB
EGAF00008067935 cram 6.3 GB
EGAF00008067936 cram 8.3 GB
EGAF00008067937 cram 7.5 GB
EGAF00008067938 cram 9.0 GB
EGAF00008067939 cram 7.4 GB
EGAF00008067940 cram 8.0 GB
EGAF00008067941 cram 7.8 GB
EGAF00008067942 cram 8.7 GB
EGAF00008067943 cram 8.1 GB
EGAF00008067944 cram 8.5 GB
EGAF00008067945 cram 6.8 GB
EGAF00008067946 cram 7.5 GB
EGAF00008067947 cram 9.9 GB
EGAF00008067948 cram 10.6 GB
EGAF00008067949 cram 9.9 GB
EGAF00008067950 cram 7.4 GB
EGAF00008067951 cram 9.1 GB
EGAF00008067952 cram 9.4 GB
EGAF00008067953 cram 11.3 GB
EGAF00008067954 cram 10.0 GB
EGAF00008067955 cram 11.3 GB
EGAF00008067956 cram 8.2 GB
EGAF00008067957 cram 9.6 GB
EGAF00008067958 cram 8.8 GB
EGAF00008067959 cram 8.3 GB
EGAF00008067960 cram 9.3 GB
EGAF00008067961 cram 10.5 GB
EGAF00008067962 cram 9.1 GB
EGAF00008067963 cram 10.9 GB
EGAF00008067964 cram 9.7 GB
EGAF00008067965 cram 10.1 GB
EGAF00008067966 cram 8.8 GB
EGAF00008067967 cram 11.2 GB
EGAF00008067968 cram 11.9 GB
EGAF00008067969 cram 8.9 GB
EGAF00008067970 cram 10.5 GB
EGAF00008067971 cram 8.7 GB
EGAF00008067972 cram 8.5 GB
EGAF00008067973 cram 9.9 GB
EGAF00008067974 cram 9.5 GB
EGAF00008067975 cram 9.8 GB
EGAF00008067976 cram 10.7 GB
EGAF00008067977 cram 8.2 GB
EGAF00008067978 cram 8.7 GB
EGAF00008067979 cram 8.9 GB
EGAF00008067980 cram 8.6 GB
EGAF00008067981 cram 12.3 GB
EGAF00008067982 cram 9.3 GB
EGAF00008067983 cram 9.3 GB
EGAF00008067984 cram 10.3 GB
EGAF00008067985 cram 12.0 GB
EGAF00008067986 cram 9.6 GB
EGAF00008067987 cram 7.1 GB
EGAF00008067988 cram 7.2 GB
EGAF00008067989 cram 17.3 GB
EGAF00008067990 cram 8.9 GB
EGAF00008067991 cram 12.0 GB
EGAF00008067992 cram 7.3 GB
EGAF00008067993 cram 7.0 GB
EGAF00008067994 cram 10.2 GB
EGAF00008067995 cram 8.2 GB
EGAF00008067996 cram 7.4 GB
EGAF00008067997 cram 11.2 GB
EGAF00008067998 cram 7.7 GB
EGAF00008067999 cram 8.5 GB
EGAF00008068000 cram 7.4 GB
EGAF00008068001 cram 7.4 GB
EGAF00008068002 cram 14.1 GB
EGAF00008068003 cram 7.7 GB
EGAF00008068004 cram 7.4 GB
EGAF00008068005 cram 8.6 GB
EGAF00008068006 cram 8.6 GB
EGAF00008068007 cram 9.5 GB
EGAF00008068008 cram 6.5 GB
EGAF00008068009 cram 7.9 GB
EGAF00008068010 cram 11.3 GB
EGAF00008068011 cram 6.1 GB
EGAF00008068012 cram 9.3 GB
EGAF00008068013 cram 10.3 GB
EGAF00008068014 cram 8.8 GB
EGAF00008068015 cram 8.6 GB
EGAF00008068016 cram 11.2 GB
EGAF00008068017 cram 8.0 GB
EGAF00008068018 cram 9.6 GB
EGAF00008068019 cram 9.4 GB
EGAF00008068020 cram 6.8 GB
EGAF00008068021 cram 7.7 GB
EGAF00008068022 cram 13.9 GB
EGAF00008068023 cram 7.8 GB
EGAF00008068024 cram 6.5 GB
EGAF00008068025 cram 5.9 GB
EGAF00008068026 cram 6.7 GB
EGAF00008068027 cram 8.9 GB
EGAF00008068028 cram 8.7 GB
EGAF00008068029 cram 8.2 GB
EGAF00008068030 cram 9.3 GB
EGAF00008068031 cram 9.5 GB
EGAF00008068032 cram 9.5 GB
EGAF00008068033 cram 9.4 GB
EGAF00008068034 cram 9.1 GB
EGAF00008068035 cram 9.1 GB
EGAF00008068036 cram 8.7 GB
EGAF00008068037 cram 8.7 GB
EGAF00008068038 cram 8.4 GB
EGAF00008068039 cram 10.1 GB
EGAF00008068040 cram 9.7 GB
EGAF00008068041 cram 8.7 GB
EGAF00008068042 cram 9.9 GB
EGAF00008068043 cram 9.9 GB
EGAF00008068044 cram 9.9 GB
EGAF00008068045 cram 8.6 GB
EGAF00008068046 cram 9.9 GB
EGAF00008068047 cram 7.0 GB
EGAF00008068048 cram 8.4 GB
EGAF00008068049 cram 7.4 GB
EGAF00008068050 cram 8.7 GB
EGAF00008068051 cram 8.9 GB
EGAF00008068052 cram 9.4 GB
EGAF00008068053 cram 7.9 GB
EGAF00008068054 cram 9.4 GB
EGAF00008068055 cram 10.0 GB
EGAF00008068056 cram 10.3 GB
EGAF00008068057 cram 9.0 GB
EGAF00008068058 cram 7.1 GB
EGAF00008068059 cram 8.3 GB
EGAF00008068060 cram 7.4 GB
EGAF00008068061 cram 11.9 GB
EGAF00008068062 cram 7.8 GB
EGAF00008068063 cram 7.7 GB
EGAF00008068064 cram 9.9 GB
EGAF00008068065 cram 7.8 GB
EGAF00008068066 cram 10.8 GB
EGAF00008068067 cram 9.8 GB
EGAF00008068068 cram 8.0 GB
EGAF00008068069 cram 12.3 GB
EGAF00008068070 cram 9.3 GB
EGAF00008068071 cram 10.8 GB
EGAF00008068072 cram 9.3 GB
EGAF00008068073 cram 10.3 GB
EGAF00008068074 cram 9.0 GB
EGAF00008068075 cram 10.7 GB
EGAF00008068076 cram 11.7 GB
EGAF00008068077 cram 10.6 GB
EGAF00008068078 cram 9.8 GB
EGAF00008068079 cram 12.0 GB
EGAF00008068080 cram 9.6 GB
EGAF00008068081 cram 8.7 GB
EGAF00008068082 cram 11.0 GB
EGAF00008068083 cram 9.5 GB
EGAF00008068084 cram 11.0 GB
EGAF00008068085 cram 9.8 GB
EGAF00008068086 cram 8.9 GB
EGAF00008068087 cram 9.3 GB
EGAF00008068088 cram 10.3 GB
EGAF00008068089 cram 9.2 GB
EGAF00008068090 cram 9.8 GB
EGAF00008068091 cram 7.9 GB
EGAF00008068092 cram 10.5 GB
EGAF00008068093 cram 8.8 GB
EGAF00008068094 cram 9.9 GB
EGAF00008068095 cram 9.5 GB
EGAF00008068096 cram 9.9 GB
EGAF00008068097 cram 10.7 GB
EGAF00008068098 cram 8.1 GB
EGAF00008068099 cram 10.5 GB
EGAF00008068100 cram 9.7 GB
EGAF00008068101 cram 11.0 GB
EGAF00008068102 cram 9.6 GB
EGAF00008068103 cram 10.6 GB
EGAF00008068104 cram 9.6 GB
EGAF00008068105 cram 8.9 GB
EGAF00008068106 cram 8.5 GB
EGAF00008068107 cram 9.4 GB
EGAF00008068108 cram 9.5 GB
EGAF00008068109 cram 12.4 GB
EGAF00008068110 cram 8.3 GB
EGAF00008068111 cram 10.2 GB
EGAF00008068112 cram 8.6 GB
EGAF00008068113 cram 10.3 GB
EGAF00008068114 cram 9.7 GB
EGAF00008068115 cram 8.9 GB
EGAF00008068116 cram 11.1 GB
EGAF00008068117 cram 8.6 GB
EGAF00008068118 cram 8.7 GB
EGAF00008068119 cram 9.7 GB
EGAF00008068120 cram 10.8 GB
EGAF00008068121 cram 10.4 GB
EGAF00008068122 cram 9.8 GB
EGAF00008068123 cram 9.1 GB
EGAF00008068124 cram 11.9 GB
EGAF00008068125 cram 10.5 GB
EGAF00008068126 cram 8.8 GB
EGAF00008068127 cram 11.3 GB
EGAF00008068128 cram 7.7 GB
EGAF00008068129 cram 9.7 GB
EGAF00008068130 cram 9.6 GB
EGAF00008068131 cram 9.9 GB
EGAF00008068132 cram 9.0 GB
EGAF00008068133 cram 7.6 GB
EGAF00008068134 cram 11.5 GB
EGAF00008068135 cram 10.7 GB
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EGAF00008068137 cram 7.9 GB
EGAF00008068138 cram 8.7 GB
EGAF00008068139 cram 8.7 GB
EGAF00008068140 cram 7.9 GB
EGAF00008068141 cram 9.5 GB
EGAF00008068142 cram 10.8 GB
EGAF00008068143 cram 9.2 GB
EGAF00008068144 cram 12.2 GB
EGAF00008068145 cram 8.4 GB
EGAF00008068146 cram 9.3 GB
EGAF00008068147 cram 17.4 GB
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EGAF00008068149 cram 7.5 GB
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EGAF00008068151 cram 8.9 GB
EGAF00008068152 cram 10.0 GB
EGAF00008068153 cram 7.9 GB
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EGAF00008068155 cram 8.4 GB
EGAF00008068156 cram 13.4 GB
EGAF00008068157 cram 9.8 GB
EGAF00008068158 cram 18.3 GB
EGAF00008068159 cram 7.2 GB
EGAF00008068160 cram 8.2 GB
EGAF00008068161 cram 12.3 GB
EGAF00008068162 cram 11.2 GB
EGAF00008068163 cram 8.1 GB
EGAF00008068164 cram 7.9 GB
EGAF00008068165 cram 8.4 GB
EGAF00008068166 cram 9.7 GB
EGAF00008068167 cram 6.7 GB
EGAF00008068168 cram 8.1 GB
EGAF00008068169 cram 8.3 GB
EGAF00008068170 cram 8.0 GB
EGAF00008068171 cram 8.0 GB
EGAF00008068172 cram 13.3 GB
EGAF00008068173 cram 9.3 GB
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EGAF00008068175 cram 9.4 GB
EGAF00008068176 cram 10.8 GB
EGAF00008068177 cram 7.5 GB
EGAF00008068178 cram 9.9 GB
EGAF00008068179 cram 15.9 GB
EGAF00008068180 cram 9.1 GB
EGAF00008068181 cram 15.5 GB
EGAF00008068182 cram 10.3 GB
EGAF00008068183 cram 17.4 GB
EGAF00008068184 cram 7.4 GB
EGAF00008068185 cram 8.2 GB
EGAF00008068186 cram 12.5 GB
EGAF00008068187 cram 10.3 GB
EGAF00008068188 cram 9.3 GB
EGAF00008068189 cram 10.1 GB
EGAF00008068190 cram 14.5 GB
EGAF00008068191 cram 14.4 GB
EGAF00008068192 cram 13.7 GB
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EGAF00008068195 cram 11.5 GB
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EGAF00008068199 cram 10.2 GB
EGAF00008068200 cram 8.0 GB
EGAF00008068201 cram 7.7 GB
EGAF00008068202 cram 12.8 GB
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EGAF00008068206 cram 9.8 GB
EGAF00008068207 cram 9.2 GB
EGAF00008068208 cram 8.6 GB
EGAF00008068209 cram 10.7 GB
EGAF00008068210 cram 7.1 GB
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EGAF00008068212 cram 8.3 GB
EGAF00008068213 cram 6.8 GB
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EGAF00008068215 cram 9.1 GB
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EGAF00008068217 cram 7.8 GB
EGAF00008068218 cram 7.6 GB
EGAF00008068219 cram 9.1 GB
EGAF00008068220 cram 8.4 GB
EGAF00008068221 cram 11.8 GB
EGAF00008068222 cram 13.6 GB
EGAF00008068223 cram 13.8 GB
EGAF00008068224 cram 9.5 GB
EGAF00008068225 cram 7.1 GB
EGAF00008068226 cram 6.9 GB
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EGAF00008068230 cram 8.7 GB
EGAF00008068231 cram 10.1 GB
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EGAF00008068244 cram 8.9 GB
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EGAF00008068247 cram 11.5 GB
EGAF00008068248 cram 8.5 GB
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EGAF00008068255 cram 9.8 GB
EGAF00008068256 cram 11.0 GB
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EGAF00008068259 cram 7.8 GB
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EGAF00008068283 cram 17.4 GB
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EGAF00008068292 cram 12.3 GB
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EGAF00008068591 cram 11.0 GB
EGAF00008068592 cram 12.0 GB
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EGAF00008068597 cram 12.8 GB
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EGAF00008068632 cram 4.2 GB
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EGAF00008068637 cram 2.8 GB
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EGAF00008068641 cram 3.9 GB
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EGAF00008068644 cram 3.6 GB
EGAF00008068645 cram 3.2 GB
EGAF00008068646 cram 2.7 GB
EGAF00008068647 cram 3.5 GB
EGAF00008068648 cram 4.6 GB
EGAF00008068649 cram 3.5 GB
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EGAF00008068651 cram 4.3 GB
EGAF00008068652 cram 3.4 GB
EGAF00008068653 cram 3.9 GB
EGAF00008068654 cram 2.6 GB
EGAF00008068655 cram 4.8 GB
EGAF00008068656 cram 4.0 GB
EGAF00008068657 cram 4.6 GB
EGAF00008068658 cram 3.6 GB
EGAF00008068659 cram 5.0 GB
EGAF00008068660 cram 3.6 GB
EGAF00008068661 cram 4.4 GB
EGAF00008068662 cram 4.7 GB
EGAF00008068663 cram 3.3 GB
EGAF00008068664 cram 4.7 GB
EGAF00008068665 cram 2.6 GB
EGAF00008068666 cram 3.2 GB
EGAF00008068667 cram 3.0 GB
EGAF00008068668 cram 2.7 GB
EGAF00008068669 cram 3.5 GB
EGAF00008068670 cram 3.7 GB
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EGAF00008068792 cram 9.1 GB
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EGAF00008068812 cram 11.0 GB
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EGAF00008068875 cram 12.6 GB
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EGAF00008068905 cram 14.0 GB
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EGAF00008069017 cram 14.5 GB
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EGAF00008069077 cram 12.3 GB
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EGAF00008069092 cram 9.9 GB
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EGAF00008069096 cram 12.5 GB
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EGAF00008069100 cram 8.0 GB
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EGAF00008069103 cram 10.0 GB
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EGAF00008069105 cram 10.3 GB
EGAF00008069106 cram 9.5 GB
EGAF00008069107 cram 8.6 GB
EGAF00008069108 cram 9.9 GB
EGAF00008069109 cram 9.0 GB
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EGAF00008069111 cram 9.2 GB
EGAF00008069112 cram 8.1 GB
EGAF00008069113 cram 8.8 GB
EGAF00008069114 cram 10.4 GB
EGAF00008069115 cram 11.6 GB
EGAF00008069116 cram 8.9 GB
EGAF00008069117 cram 9.3 GB
EGAF00008069118 cram 10.3 GB
EGAF00008069119 cram 8.2 GB
EGAF00008069120 cram 8.7 GB
EGAF00008069121 cram 8.2 GB
EGAF00008069122 cram 9.9 GB
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EGAF00008069124 cram 9.6 GB
EGAF00008069125 cram 10.5 GB
EGAF00008069126 cram 9.3 GB
EGAF00008069127 cram 9.4 GB
EGAF00008069128 cram 10.8 GB
EGAF00008069129 cram 7.9 GB
EGAF00008069130 cram 9.1 GB
EGAF00008069131 cram 10.8 GB
EGAF00008069132 cram 8.8 GB
EGAF00008069133 cram 11.0 GB
EGAF00008069134 cram 8.1 GB
EGAF00008069135 cram 10.0 GB
EGAF00008069136 cram 9.7 GB
EGAF00008069137 cram 9.3 GB
EGAF00008069138 cram 8.7 GB
EGAF00008069139 cram 9.5 GB
EGAF00008069140 cram 10.6 GB
EGAF00008069141 cram 10.9 GB
EGAF00008069142 cram 9.1 GB
EGAF00008069143 cram 11.6 GB
EGAF00008069144 cram 9.1 GB
EGAF00008069145 cram 9.2 GB
EGAF00008069146 cram 9.6 GB
EGAF00008069147 cram 10.5 GB
EGAF00008069148 cram 11.0 GB
EGAF00008069149 cram 10.7 GB
EGAF00008069150 cram 8.4 GB
EGAF00008069151 cram 12.2 GB
EGAF00008069152 cram 10.0 GB
EGAF00008069153 cram 10.3 GB
EGAF00008069154 cram 9.6 GB
EGAF00008069155 cram 10.3 GB
EGAF00008069156 cram 11.4 GB
EGAF00008069157 cram 12.3 GB
EGAF00008069158 cram 10.3 GB
EGAF00008069159 cram 9.7 GB
EGAF00008069160 cram 10.4 GB
EGAF00008069161 cram 8.9 GB
EGAF00008069162 cram 8.6 GB
EGAF00008069163 cram 10.1 GB
EGAF00008069164 cram 10.5 GB
EGAF00008069165 cram 11.2 GB
EGAF00008069166 cram 10.7 GB
EGAF00008069167 cram 10.2 GB
EGAF00008069168 cram 9.2 GB
EGAF00008069169 cram 9.9 GB
EGAF00008069170 cram 9.7 GB
EGAF00008069171 cram 9.0 GB
EGAF00008069172 cram 9.8 GB
EGAF00008069173 cram 7.2 GB
EGAF00008069174 cram 10.5 GB
EGAF00008069175 cram 10.4 GB
EGAF00008069176 cram 8.3 GB
EGAF00008069177 cram 9.7 GB
EGAF00008069178 cram 10.6 GB
EGAF00008069179 cram 10.4 GB
EGAF00008069180 cram 10.8 GB
EGAF00008069181 cram 9.9 GB
EGAF00008069182 cram 11.0 GB
EGAF00008069183 cram 10.1 GB
EGAF00008069184 cram 11.3 GB
EGAF00008069185 cram 9.9 GB
EGAF00008069186 cram 9.6 GB
EGAF00008069187 cram 10.1 GB
EGAF00008069188 cram 10.5 GB
EGAF00008069189 cram 11.4 GB
EGAF00008069190 cram 10.8 GB
EGAF00008069191 cram 9.7 GB
EGAF00008069192 cram 12.6 GB
EGAF00008069193 cram 10.5 GB
EGAF00008069194 cram 10.3 GB
EGAF00008069195 cram 10.2 GB
EGAF00008069196 cram 13.2 GB
EGAF00008069197 cram 11.0 GB
EGAF00008069198 cram 10.1 GB
EGAF00008069199 cram 10.4 GB
EGAF00008069200 cram 10.8 GB
EGAF00008069201 cram 9.7 GB
EGAF00008069202 cram 11.4 GB
EGAF00008069203 cram 10.6 GB
EGAF00008069204 cram 11.7 GB
EGAF00008069205 cram 10.2 GB
EGAF00008069206 cram 9.8 GB
EGAF00008069207 cram 8.8 GB
EGAF00008069208 cram 11.5 GB
EGAF00008069209 cram 10.3 GB
EGAF00008069210 cram 13.9 GB
EGAF00008069211 cram 12.1 GB
EGAF00008069212 cram 11.6 GB
EGAF00008069213 cram 11.7 GB
EGAF00008069214 cram 10.1 GB
EGAF00008069215 cram 11.3 GB
EGAF00008069216 cram 11.5 GB
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EGAF00008069218 cram 13.5 GB
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EGAF00008069220 cram 9.3 GB
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EGAF00008069222 cram 9.0 GB
EGAF00008069223 cram 11.7 GB
EGAF00008069224 cram 10.4 GB
EGAF00008069225 cram 11.8 GB
EGAF00008069226 cram 9.6 GB
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EGAF00008069233 cram 10.9 GB
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EGAF00008069236 cram 10.8 GB
EGAF00008069237 cram 8.8 GB
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EGAF00008069239 cram 10.4 GB
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EGAF00008069246 cram 13.3 GB
EGAF00008069247 cram 11.6 GB
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EGAF00008069252 cram 11.8 GB
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EGAF00008069254 cram 9.9 GB
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EGAF00008069257 cram 8.8 GB
EGAF00008069258 cram 6.7 GB
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EGAF00008069268 cram 11.5 GB
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EGAF00008069284 cram 11.1 GB
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EGAF00008069314 cram 9.7 GB
EGAF00008069315 cram 12.1 GB
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EGAF00008069344 cram 9.6 GB
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EGAF00008069346 cram 9.9 GB
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EGAF00008069349 cram 9.6 GB
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EGAF00008069351 cram 12.4 GB
EGAF00008069352 cram 7.7 GB
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EGAF00008069357 cram 9.3 GB
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EGAF00008069377 cram 7.8 GB
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EGAF00008069533 cram 15.5 GB
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EGAF00008069663 cram 8.9 GB
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EGAF00008069666 cram 9.5 GB
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EGAF00008069681 cram 7.1 GB
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EGAF00008069683 cram 6.5 GB
EGAF00008069684 cram 7.9 GB
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EGAF00008069687 cram 13.5 GB
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EGAF00008069696 cram 4.8 GB
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EGAF00008069699 cram 7.9 GB
EGAF00008069700 cram 9.9 GB
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EGAF00008069702 cram 9.1 GB
EGAF00008069703 cram 10.0 GB
EGAF00008069704 cram 12.5 GB
EGAF00008069705 cram 9.5 GB
EGAF00008069706 cram 8.2 GB
EGAF00008069707 cram 9.6 GB
EGAF00008069708 cram 10.3 GB
EGAF00008069709 cram 9.3 GB
EGAF00008069710 cram 9.9 GB
EGAF00008069711 cram 10.0 GB
EGAF00008069712 cram 8.7 GB
EGAF00008069713 cram 10.4 GB
EGAF00008069714 cram 10.3 GB
EGAF00008069715 cram 9.4 GB
EGAF00008069716 cram 9.0 GB
EGAF00008069717 cram 10.2 GB
EGAF00008069718 cram 9.5 GB
EGAF00008069719 cram 11.1 GB
EGAF00008069720 cram 13.4 GB
EGAF00008069721 cram 9.1 GB
EGAF00008069722 cram 8.3 GB
EGAF00008069723 cram 10.5 GB
EGAF00008069724 cram 8.0 GB
EGAF00008069725 cram 8.2 GB
EGAF00008069726 cram 7.9 GB
EGAF00008069727 cram 8.8 GB
EGAF00008069728 cram 9.0 GB
EGAF00008069729 cram 8.8 GB
EGAF00008069730 cram 11.0 GB
EGAF00008069731 cram 9.5 GB
EGAF00008069732 cram 8.0 GB
EGAF00008069733 cram 7.9 GB
EGAF00008069734 cram 8.3 GB
EGAF00008069735 cram 8.6 GB
EGAF00008069736 cram 8.8 GB
EGAF00008069737 cram 10.8 GB
EGAF00008069738 cram 7.8 GB
EGAF00008069739 cram 9.2 GB
EGAF00008069740 cram 8.8 GB
EGAF00008069741 cram 8.4 GB
EGAF00008069742 cram 8.9 GB
EGAF00008069743 cram 10.4 GB
EGAF00008069744 cram 9.6 GB
EGAF00008069745 cram 7.8 GB
EGAF00008069746 cram 9.0 GB
EGAF00008069747 cram 10.4 GB
EGAF00008069748 cram 9.4 GB
EGAF00008069749 cram 9.3 GB
EGAF00008069750 cram 8.3 GB
EGAF00008069751 cram 9.5 GB
EGAF00008069752 cram 8.0 GB
EGAF00008069753 cram 8.3 GB
EGAF00008069754 cram 9.8 GB
EGAF00008069755 cram 9.3 GB
EGAF00008069756 cram 8.5 GB
EGAF00008069757 cram 9.1 GB
EGAF00008069758 cram 10.6 GB
EGAF00008069759 cram 9.4 GB
EGAF00008069760 cram 9.9 GB
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EGAF00008069766 cram 9.9 GB
EGAF00008069767 cram 5.8 GB
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EGAF00008069770 cram 11.6 GB
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EGAF00008069778 cram 6.9 GB
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EGAF00008069785 cram 9.7 GB
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EGAF00008069790 cram 7.9 GB
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EGAF00008069805 cram 9.9 GB
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EGAF00008069814 cram 11.3 GB
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EGAF00008069817 cram 12.3 GB
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EGAF00008069820 cram 9.5 GB
EGAF00008069821 cram 10.7 GB
EGAF00008069822 cram 12.1 GB
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EGAF00008069825 cram 9.9 GB
EGAF00008069826 cram 9.5 GB
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EGAF00008069828 cram 11.7 GB
EGAF00008069829 cram 10.5 GB
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EGAF00008069832 cram 9.1 GB
EGAF00008069833 cram 12.3 GB
EGAF00008069834 cram 8.7 GB
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EGAF00008069836 cram 10.1 GB
EGAF00008069837 cram 13.6 GB
EGAF00008069838 cram 12.4 GB
EGAF00008069839 cram 10.7 GB
EGAF00008069840 cram 12.6 GB
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EGAF00008069843 cram 10.9 GB
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EGAF00008069858 cram 7.4 GB
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EGAF00008069872 cram 8.9 GB
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EGAF00008069874 cram 9.7 GB
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EGAF00008069923 cram 11.8 GB
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EGAF00008069928 cram 12.3 GB
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EGAF00008069957 cram 9.8 GB
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EGAF00008070229 cram 4.2 GB
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EGAF00008070238 cram 3.8 GB
EGAF00008070239 cram 2.9 GB
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EGAF00008070241 cram 4.9 GB
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EGAF00008070246 cram 2.9 GB
EGAF00008070247 cram 3.2 GB
EGAF00008070248 cram 4.4 GB
EGAF00008070249 cram 4.3 GB
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EGAF00008070251 cram 2.4 GB
EGAF00008070252 cram 3.0 GB
EGAF00008070253 cram 3.7 GB
EGAF00008070254 cram 2.6 GB
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EGAF00008070256 cram 3.8 GB
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EGAF00008070258 cram 2.3 GB
EGAF00008070259 cram 3.6 GB
EGAF00008070260 cram 2.6 GB
EGAF00008070261 cram 2.3 GB
EGAF00008070262 cram 2.5 GB
EGAF00008070263 cram 3.4 GB
EGAF00008070264 cram 2.7 GB
EGAF00008070265 cram 3.6 GB
EGAF00008070266 cram 3.5 GB
EGAF00008070267 cram 3.3 GB
EGAF00008070268 cram 3.1 GB
EGAF00008070269 cram 3.8 GB
EGAF00008070270 cram 3.8 GB
EGAF00008070271 cram 3.3 GB
EGAF00008070272 cram 4.7 GB
EGAF00008070273 cram 3.8 GB
EGAF00008070274 cram 3.4 GB
EGAF00008070275 cram 3.3 GB
EGAF00008070276 cram 3.8 GB
EGAF00008070277 cram 3.1 GB
EGAF00008070278 cram 2.9 GB
EGAF00008070279 cram 3.7 GB
EGAF00008070280 cram 3.3 GB
EGAF00008070281 cram 3.9 GB
EGAF00008070282 cram 2.5 GB
EGAF00008070283 cram 4.2 GB
EGAF00008070284 cram 3.6 GB
EGAF00008070285 cram 2.5 GB
EGAF00008070286 cram 3.0 GB
EGAF00008070287 cram 3.3 GB
EGAF00008070288 cram 2.9 GB
EGAF00008070289 cram 3.1 GB
EGAF00008070290 cram 3.4 GB
EGAF00008070291 cram 4.0 GB
EGAF00008070292 cram 3.8 GB
EGAF00008070293 cram 2.9 GB
EGAF00008070294 cram 2.9 GB
EGAF00008070295 cram 4.9 GB
EGAF00008070296 cram 3.0 GB
EGAF00008070297 cram 4.0 GB
EGAF00008070298 cram 2.6 GB
EGAF00008070299 cram 4.2 GB
EGAF00008070300 cram 2.9 GB
EGAF00008070301 cram 3.3 GB
EGAF00008070302 cram 4.4 GB
EGAF00008070303 cram 4.4 GB
EGAF00008070304 cram 2.8 GB
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EGAF00008070742 cram 1.8 GB
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EGAF00008070755 cram 2.0 GB
EGAF00008070756 cram 1.2 GB
EGAF00008070757 cram 2.6 GB
EGAF00008070758 cram 2.4 GB
EGAF00008070759 cram 1.8 GB
EGAF00008070760 cram 2.4 GB