Healthy human B-lymphopoiesis RNA-Seq reference using FACS sorted bone marrow aspirates
To define a transcriptomic reference of human B lymphopoiesis, bone marrow aspirates were obtained from n=4 healthy donors (study registration DRKS00023583). After immunodensity cell separation, samples were FACS-sorted into 7 established lymphopoietic differentiation stages. RNA was extracted from 5,000-320,000 cells per differentiation stage and subjected to ultra-low-input RNA sequencing after generation of stranded sequencing libraries.
- 28 samples
- DAC: EGAC00001003270
- Technology: Illumina NovaSeq 6000
Data will be provided for research purpose exclusively based on the institution's data transfer agreement.
Data will be provided for research purpose exclusively based on the institution's data transfer agreement.
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
Study ID | Study Title | Study Type |
---|---|---|
EGAS00001007305 | RNASeq |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.