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Whole blood RNA sequencing of individuals from Nepal

This study utilized blood samples collected in Lalitpur, Nepal as part of the Strategic Typhoid Alliance across Africa and Asia (STRATAA) study. The dataset comprises whole blood RNAseq of 376 febrile individuals from Nepal. Blood was collected in PAXgene tubes and sent to Monash University (Melbourne, Australia) where RNA was extracted using the PAXgene Blood RNA kit before being sent to the Wellcome Sanger Institute (Hinxton, UK) for sequencing. Library prep used NEBNext Ultra II RNA custom kits on an Agilent Bravo WS automation platform with poly(A) pulldown. After PCR, plates were purified using Agencourt AMPure XP SPRI beads and libraries were quantified using Biotium Accuclear Ultra high sensitivity dsDNA Quantitative kits. Pooled libraries were normalised to 2.8 nM. Samples were globin depleted using KAPA RNA HyperPrep with RiboErase. Libraries were then subjected to 2x100bp paired-end sequencing on Illumina NovaSeq. Each library was sequenced to an average of 80 million reads. The STRATAA study was approved by the Nepal Health Research Council (NHRC, ref 283 306/2015) and OxTREC (Oxford Tropical Research Ethics Committee, ref 39-15). All participants provided informed consent for human genetic tests.

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Study ID Study Title Study Type
EGAS00001003967 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
EGAF00008122362 cram 2.2 GB Report
EGAF00008122363 cram 2.5 GB Report
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EGAF00008122365 cram 3.3 GB Report
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EGAF00008122367 cram 3.3 GB Report
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EGAF00008122371 cram 2.5 GB Report
EGAF00008122372 cram 3.0 GB Report
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EGAF00008122379 cram 2.6 GB Report
EGAF00008122380 cram 2.7 GB Report
EGAF00008122381 cram 2.4 GB Report
EGAF00008122382 cram 3.6 GB Report
EGAF00008122383 cram 2.9 GB Report
EGAF00008122384 cram 2.2 GB Report
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EGAF00008122387 cram 3.2 GB Report
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EGAF00008122548 cram 2.7 GB Report
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EGAF00008122590 cram 2.5 GB Report
EGAF00008122591 cram 2.5 GB Report
EGAF00008122592 cram 2.9 GB Report
EGAF00008122593 cram 1.9 GB Report
EGAF00008122594 cram 2.7 GB Report
EGAF00008122595 cram 2.9 GB Report
EGAF00008122596 cram 2.2 GB Report
EGAF00008122597 cram 2.7 GB Report
EGAF00008122598 cram 2.6 GB Report
EGAF00008122599 cram 2.8 GB Report
EGAF00008122600 cram 3.3 GB Report
EGAF00008122601 cram 2.5 GB Report
EGAF00008122602 cram 2.4 GB Report
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EGAF00008122608 cram 2.6 GB Report
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EGAF00008122611 cram 3.2 GB Report
EGAF00008122612 cram 3.1 GB Report
EGAF00008122613 cram 2.5 GB Report
EGAF00008122614 cram 1.5 GB Report
EGAF00008122615 cram 1.9 GB Report
EGAF00008122616 cram 1.9 GB Report
EGAF00008122617 cram 1.5 GB Report
EGAF00008122618 cram 1.3 GB Report
EGAF00008122619 cram 2.4 GB Report
EGAF00008122620 cram 2.0 GB Report
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EGAF00008122622 cram 2.0 GB Report
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EGAF00008122688 cram 3.5 GB Report
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EGAF00008122690 cram 541.3 kB Report
EGAF00008122691 cram 728.6 kB Report
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EGAF00008122693 cram 357.9 kB Report
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EGAF00008122736 cram 2.8 GB Report
EGAF00008122737 cram 2.8 GB Report
376 Files (1.0 TB)