Single-cell RNA sequencing of SarBC-01 treated with Dexamethasone vs DMSO, with or without Matrigel.

This dataset contains raw FASTQ files from single cell RNA sequencing of SarBC-01 cells treated with Dexamethasone vs DMSO, with or without Matrigel, processed with MULTI-seq. Cells from 4 different culture conditions (+Mat/Dex, +Mat/DMSO, -Mat/Dex, -Mat/DMSO) were harvested, processed for multiplexing using the MULTI-seq protocol and loaded in a Chromium Single Cell 3ʹ GEM Library and Gel Bead Kit v3 (10x Genomics). Gene expression (cDNA) and MULTI-seq libraries were prepared according to the manufacturers’ protocol of Chromium Next GEM Single Cell 3’ reagents Kits v3.1 (Dual Index). Finally, cDNA and MULTI-seq libraries were analyzed using an Agilent Bioanalyzer (DNA High Sensitivity kit) and sequenced on a NovaSeq6000 (S2 flow cell) platform. MULTISEQ BARCODES: TTAGCCAG => Matrigel/DMSO CCACAATG => Matrigel/Dexamethasone GCACACGC => noMatrigel/DMSO AGAGAGAG => noMatrigel/Dexamethasone

2 samples
DAC: EGAC00001002981
Technology: Illumina NovaSeq 6000

Access Policy1 Study History Files

Metadata

Request Access

Data Access Policy of Datasets controlled by MF-DAC

ATTENTION: Please check the following policy and the documents/information to provide in order to get access to the requested datasets. You can find the governing policy on Zenodo under the link: https://zenodo.org/records/12517622 Please provide all information as requested in the document to the MF-DAC. Additionally, you can find more information about the Data Access Committee of the Medical Faculty of the University of Basel (MF-DAC) under the following link on Zenodo: https://zenodo.org/records/10137776

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID	Study Title	Study Type
EGAS00001007430	Patient-derived organoids identify tailored therapeutic options and determinants of plasticity in sarcomatoid urothelial bladder cancer	Cancer Genomics

Updated policy to match request of MF-DAC. 12/07/2023
Updated policy to match request of MF-DAC. 12/07/2023
Set back the correct policy number. 12/07/2023
Dataset Released 12/07/2023

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID	File Type	Size
EGAF00008157589	fastq.gz	14.5 GB
EGAF00008157590	fastq.gz	32.0 GB
EGAF00008157591	fastq.gz	2.0 GB
EGAF00008157592	fastq.gz	4.1 GB
4 Files (52.6 GB)