Influence of culture media on airway differentiation at the Air-Liquid Interface
Single-cell dataset of human airways after in vitro regeneration at the Air-Liquid Interface. Samples from 2 donors were used for the establishment of Human Bronchial Epithelial Cell cultures (HBECs) and were analyzed during proliferation, at the onset of differentiation (ALI0) and after full differentiation (ALI28), in 4 distinct culture media. Single-cell RNAseq was performed with 10x Genomics 3’ v2 RNA-seq using hashtag antibodies for multiplexing, and sequenced on a NextSeq 500. Samples from 3 additional donors were used for the establishment of Human Nasal Epithelial Cell cultures (HNECs) and were analyzed during proliferation in three distinct culture media. Single-cell RNAseq was performed with 10x Genomics 3’ v3 RNA-seq without multiplexing, and sequenced on a NextSeq 2000.
- 11 samples
- DAC: EGAC00001003434
- Technologies: NextSeq 500, unspecified
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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| EGAS00001007572 | Other |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Located in | |
|---|---|---|---|---|
| EGAF00008247536 | bam | 6.1 GB | ||
| EGAF00008247537 | bam | 10.3 GB | ||
| EGAF00008247538 | bam | 11.4 GB | ||
| EGAF00008247539 | bam | 8.6 GB | ||
| EGAF00008247540 | bam | 9.2 GB | ||
| EGAF00008247541 | bam | 7.7 GB | ||
| EGAF00008247542 | bam | 22.5 GB | ||
| EGAF00008247543 | bam | 27.6 GB | ||
| EGAF00008247544 | bam | 2.7 GB | ||
| EGAF00008247545 | bam | 27.2 GB | ||
| EGAF00008247546 | bam | 3.3 GB | ||
| 11 Files (136.6 GB) | ||||