RNA-seq dataset: Single-cell spatial analysis of pediatric high-grade glioma reveals a novel population of immunosuppressive and tumor-promoting SPP1+/GPNMB+ myeloid cells
Our study utilizes two novel techniques, cyclical immunofluorescence imaging and single-cell spatial transcriptomics, to spatially map the tumor microenvironment of a large sample set of pediatric high-grade gliomas (pHGG). Using these methods, we have identified an abundant immunosuppressive myeloid cell population that has not been described in the context of pediatric high-grade gliomas. We validate our findings using an in vitro assay, spatial analysis on additional pHGG biopsies, independent spatial transcriptomic data, bulk RNA sequencing data of an expanded cohort of in-house patients, and publicly available bulk RNA sequencing data of an even larger cohort of pHGG patients.
- 17/12/2024
- 1 sample
- DAC: EGAC00001001864
DUO:0000007 version: 2021-02-23
disease specific research
This data use permission indicates that use is allowed provided it is related to the specified disease.
ModifiersMONDO_0006517
Data Access Agreement of data controlled by the Data Access Commitee of the Princess Maxima Center for Pediatric Oncology
The Princess Maxima Center for pediatric oncology default access policy to enable re-production of research and re-use of data for scientific purposes. Please contact the DAC (EGAC00001001864) for the most up to date policies.
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
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| EGAS00001005312 | Other | |
| EGAS00001006723 | Other | |
| EGAS00001007099 | Other |
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- Dataset Released
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Quality Report |
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| EGAF00005299819 | cram | 13.6 GB |
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| EGAF00005299939 | cram | 10.6 GB |
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| EGAF00005488183 | cram | 4.7 GB |
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| EGAF00005488211 | cram | 5.4 GB |
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| EGAF00007984644 | cram | 6.3 GB |
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| EGAF00007984651 | cram | 7.2 GB |
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| EGAF00007984659 | cram | 6.5 GB |
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| EGAF00007984710 | cram | 7.4 GB |
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| EGAF00007984718 | cram | 4.5 GB |
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| EGAF00007984722 | cram | 6.0 GB |
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| EGAF00007984763 | cram | 6.3 GB |
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| EGAF00007984807 | cram | 4.5 GB |
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| EGAF00008634688 | cram | 4.8 GB |
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| EGAF00008634689 | cram | 6.6 GB |
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| EGAF00008634690 | cram | 6.5 GB |
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| EGAF00008634691 | cram | 6.3 GB |
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| EGAF00008634692 | cram | 5.9 GB |
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| EGAF00008634693 | cram | 6.2 GB |
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| EGAF00008634694 | cram | 6.8 GB |
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| EGAF00008634695 | cram | 10.5 GB |
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| EGAF00008634696 | cram | 7.7 GB |
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| EGAF00008634697 | cram | 7.7 GB |
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| EGAF00008634698 | cram | 3.5 GB |
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| EGAF00008634699 | cram | 5.9 GB |
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| EGAF00008634700 | cram | 4.5 GB |
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| EGAF00008634701 | cram | 4.1 GB |
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| EGAF00008634702 | cram | 6.5 GB |
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| EGAF00008634703 | cram | 6.4 GB |
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| EGAF00008634704 | cram | 5.4 GB |
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| EGAF00008634705 | cram | 7.0 GB |
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| EGAF00008634706 | cram | 5.5 GB |
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| EGAF00008634707 | cram | 7.7 GB |
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| EGAF00008634708 | cram | 4.0 GB |
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| EGAF00008634709 | cram | 8.0 GB |
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| EGAF00008634710 | cram | 4.6 GB |
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| EGAF00008634711 | cram | 8.1 GB |
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| EGAF00008634712 | cram | 7.6 GB |
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| EGAF00008634713 | cram | 5.8 GB |
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| EGAF00008634714 | cram | 5.8 GB |
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| EGAF00008634715 | cram | 6.2 GB |
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| EGAF00008634716 | cram | 6.0 GB |
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| EGAF00008634717 | cram | 4.5 GB |
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| EGAF00008634718 | cram | 4.7 GB |
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| EGAF00008634719 | cram | 4.9 GB |
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| EGAF00008634720 | cram | 4.8 GB |
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| EGAF00008634721 | cram | 5.4 GB |
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| EGAF00008634722 | cram | 4.7 GB |
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| EGAF00008634723 | cram | 10.1 GB |
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| EGAF00008634724 | cram | 14.0 GB |
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| EGAF00008634725 | cram | 6.3 GB |
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| EGAF00008634726 | cram | 7.0 GB |
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| EGAF00008634727 | cram | 8.7 GB |
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| EGAF00008634728 | cram | 7.8 GB |
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| EGAF00008634729 | cram | 9.4 GB |
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| EGAF00008634730 | cram | 9.1 GB |
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| EGAF00008634731 | cram | 6.5 GB |
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| EGAF00008634732 | cram | 11.1 GB |
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| EGAF00008634733 | cram | 9.8 GB |
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| EGAF00008634734 | cram | 7.1 GB |
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| EGAF00008634735 | cram | 6.9 GB |
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| EGAF00008634736 | cram | 8.8 GB |
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| EGAF00008634737 | cram | 8.6 GB |
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| EGAF00008634738 | cram | 10.2 GB |
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| EGAF00008634739 | cram | 8.8 GB |
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| EGAF00008634740 | cram | 7.1 GB |
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| EGAF00008634741 | cram | 9.0 GB |
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| EGAF00008634742 | cram | 7.1 GB |
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| EGAF00008634743 | cram | 12.4 GB |
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| EGAF00008634744 | cram | 9.3 GB |
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| EGAF00008634745 | cram | 9.4 GB |
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| 70 Files (498.2 GB) | ||||