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Pooled Single-Cell RNA-Seq of iPSC-Derived Neural Stem Cells from ADHD and Control Individuals

This dataset contains FASTQ files from pooled single-cell RNA sequencing (scRNA-seq) of neural stem cells (NSCs) derived from induced pluripotent stem cells (iPSCs). The iPSCs were reprogrammed using the non-integrative Sendai virus system (CytoTune-iPS 2.0), introducing OCT3/4, SOX2, KLF4, and cMYC. Donor somatic cells included keratinocytes, PBMCs, and lymphoblastoid cell lines from individuals with ADHD and age-matched healthy controls (6–18 years). iPSCs underwent standard quality control, including immunocytochemistry, qRT-PCR, mycoplasma testing, and SNP genotyping. NSCs were produced via neural induction and validated by qRT-PCR and immunostaining for PAX6, SOX2, NESTIN, TUJ1, and FOXG1. Sequencing was performed on the Illumina NovaSeq 6000.

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Single-Cell NSC Policy – ADHD and Control iPSC Study

Data generated from this study may only be accessed by qualified researchers for use in ethically approved projects related to neurodevelopmental disorders or related biomedical research. All users must sign a Data Access Agreement (DAA) that outlines obligations including data confidentiality, secure storage, non-identifiability, and use restrictions aligned with participant consent. Data must not be used for commercial purposes or shared with unauthorized parties. Re-identification attempts are strictly prohibited. Requests will be reviewed by the Data Access Committee for compliance with ethical, legal, and institutional guidelines.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001008169 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
EGAF00008780324 fastq.gz 1.1 GB
EGAF00008780325 fastq.gz 1.2 GB
EGAF00008780326 fastq.gz 5.3 GB
EGAF00008780327 fastq.gz 12.7 GB
EGAF00008780328 fastq.gz 1.2 GB
EGAF00008780329 fastq.gz 1.2 GB
EGAF00008780330 fastq.gz 5.5 GB
EGAF00008780331 fastq.gz 13.1 GB
EGAF00008780332 fastq.gz 1.6 GB
EGAF00008780333 fastq.gz 1.7 GB
EGAF00008780334 fastq.gz 6.6 GB
EGAF00008780335 fastq.gz 15.9 GB
EGAF00008780336 fastq.gz 1.6 GB
EGAF00008780337 fastq.gz 1.7 GB
EGAF00008780338 fastq.gz 6.6 GB
EGAF00008780339 fastq.gz 15.8 GB
16 Files (92.9 GB)