Pooled Single-Cell RNA-Seq of iPSC-Derived Neural Stem Cells from ADHD and Control Individuals
This dataset contains FASTQ files from pooled single-cell RNA sequencing (scRNA-seq) of neural stem cells (NSCs) derived from induced pluripotent stem cells (iPSCs). The iPSCs were reprogrammed using the non-integrative Sendai virus system (CytoTune-iPS 2.0), introducing OCT3/4, SOX2, KLF4, and cMYC. Donor somatic cells included keratinocytes, PBMCs, and lymphoblastoid cell lines from individuals with ADHD and age-matched healthy controls (6–18 years). iPSCs underwent standard quality control, including immunocytochemistry, qRT-PCR, mycoplasma testing, and SNP genotyping. NSCs were produced via neural induction and validated by qRT-PCR and immunostaining for PAX6, SOX2, NESTIN, TUJ1, and FOXG1. Sequencing was performed on the Illumina NovaSeq 6000.
- 08/07/2025
- 2 samples
- DAC: EGAC00001003572
- Technology: Illumina NovaSeq 6000
Single-Cell NSC Policy – ADHD and Control iPSC Study
Data generated from this study may only be accessed by qualified researchers for use in ethically approved projects related to neurodevelopmental disorders or related biomedical research. All users must sign a Data Access Agreement (DAA) that outlines obligations including data confidentiality, secure storage, non-identifiability, and use restrictions aligned with participant consent. Data must not be used for commercial purposes or shared with unauthorized parties. Re-identification attempts are strictly prohibited. Requests will be reviewed by the Data Access Committee for compliance with ethical, legal, and institutional guidelines.
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| EGAS00001008169 | Other |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Quality Report |
Located in
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| EGAF00008780324 | fastq.gz | 1.1 GB |
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| EGAF00008780325 | fastq.gz | 1.2 GB |
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| EGAF00008780326 | fastq.gz | 5.3 GB |
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| EGAF00008780327 | fastq.gz | 12.7 GB |
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| EGAF00008780328 | fastq.gz | 1.2 GB |
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| EGAF00008780329 | fastq.gz | 1.2 GB |
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| EGAF00008780330 | fastq.gz | 5.5 GB |
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| EGAF00008780331 | fastq.gz | 13.1 GB |
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| EGAF00008780332 | fastq.gz | 1.6 GB |
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| EGAF00008780333 | fastq.gz | 1.7 GB |
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| EGAF00008780334 | fastq.gz | 6.6 GB |
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| EGAF00008780335 | fastq.gz | 15.9 GB |
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| EGAF00008780336 | fastq.gz | 1.6 GB |
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| EGAF00008780337 | fastq.gz | 1.7 GB |
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| EGAF00008780338 | fastq.gz | 6.6 GB |
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| EGAF00008780339 | fastq.gz | 15.8 GB |
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| 16 Files (92.9 GB) | ||||
