On-target mutations confer resistance to WRN helicase inhibitors in Microsatellite Unstable Cancer Cells
This study investigates genetic dependencies and resistance mechanisms to Werner helicase inhibitors (WRNi) in microsatellite-unstable (MSI) cancer models. To define determinants of WRN dependency and acquired resistance, we performed genome-wide CRISPR-Cas9 knockout screens, CRISPR base-editing mutagenesis screens targeting WRN, targeted sequencing of resistance-associated variants, bulk RNA sequencing, and whole-genome sequencing. The datasets deposited in EGA comprise raw sequencing files from CRISPR-Cas9 knockout screens, CRISPR base-editing screens, targeted DNA sequencing, RNA-seq experiments, and whole-genome sequencing of parental and drug-treated cell populations, including models with spontaneously acquired resistance. These data enable investigation of WRN synthetic lethality, genetic modifiers of WRNi response, and genomic alterations emerging under selective pressure.
- 23/02/2026
- 46 samples
- DAC: EGAC00001000000
- Technologies: Illumina HiSeq 2000, Illumina HiSeq 2500, Illumina NovaSeq 6000
DUO:0000019 version: 2021-02-23
publication required
This data use modifier indicates that requestor agrees to make results of studies using the data available to the larger scientific community.
DUO:0000026 version: 2021-02-23
user specific restriction
This data use modifier indicates that use is limited to use by approved users.
DUO:0000028 version: 2021-02-23
institution specific restriction
This data use modifier indicates that use is limited to use within an approved institution.
DUO:0000042 version: 2021-02-23
general research use
This data use permission indicates that use is allowed for general research use for any research purpose.
Wellcome Trust Sanger Institute Cancer Genome Group Data Sharing Policy
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| EGAS00001000828 | Cancer Genomics | |
| EGAS00001001932 | Cancer Genomics | |
| EGAS00001004178 | Whole Genome Sequencing | |
| EGAS00001004180 | Cancer Genomics | |
| EGAS00001006872 | Cancer Genomics |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Quality Report |
Located in
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|---|---|---|---|---|
| EGAF00000684738 | cram | 9.0 GB | Report |
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| EGAF00003057108 | cram | 191.3 MB |
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| EGAF00003057109 | cram | 175.0 MB |
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| EGAF00003057110 | cram | 187.9 MB |
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| EGAF00003057116 | cram | 169.2 MB |
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| EGAF00003057121 | cram | 188.4 MB |
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| EGAF00003057122 | cram | 172.6 MB |
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| EGAF00003057123 | cram | 185.1 MB |
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| EGAF00003057129 | cram | 175.0 MB |
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| EGAF00003057134 | cram | 194.7 MB |
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| EGAF00003057135 | cram | 178.1 MB |
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| EGAF00003057136 | cram | 191.0 MB |
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| EGAF00003057284 | cram | 143.3 MB |
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| EGAF00003057289 | cram | 159.7 MB |
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| EGAF00003057290 | cram | 146.2 MB |
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| EGAF00003057291 | cram | 156.9 MB |
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| EGAF00003057297 | cram | 140.0 MB |
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| EGAF00003057302 | cram | 156.1 MB |
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| EGAF00003057303 | cram | 142.9 MB |
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| EGAF00003057304 | cram | 153.5 MB |
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| EGAF00003057711 | cram | 168.1 MB |
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| EGAF00003057716 | cram | 187.1 MB |
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| EGAF00003057717 | cram | 171.4 MB |
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| EGAF00003057718 | cram | 183.8 MB |
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| EGAF00003057724 | cram | 171.9 MB |
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| EGAF00004855908 | cram | 682.4 MB |
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| EGAF00004855909 | cram | 718.5 MB |
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| EGAF00004855910 | cram | 827.6 MB |
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| EGAF00004855911 | cram | 775.7 MB |
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| EGAF00004855912 | cram | 695.5 MB |
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| EGAF00004855913 | cram | 731.3 MB |
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| EGAF00004855914 | cram | 840.1 MB |
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| EGAF00004855915 | cram | 791.0 MB |
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| EGAF00008265515 | cram | 67.5 MB |
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| EGAF00008265516 | cram | 73.4 MB |
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| EGAF00008265518 | cram | 77.1 MB |
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| EGAF00008265520 | cram | 65.9 MB |
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| EGAF00008265524 | cram | 81.3 MB |
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| EGAF00008265527 | cram | 67.9 MB |
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| EGAF00008265529 | cram | 86.5 MB |
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| EGAF00008265531 | cram | 84.6 MB |
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| EGAF00008265538 | cram | 79.1 MB |
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| EGAF00008265540 | cram | 69.8 MB |
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| EGAF00008265541 | cram | 75.8 MB |
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| EGAF00008265543 | cram | 79.9 MB |
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| EGAF00008265545 | cram | 67.9 MB |
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| EGAF00008265549 | cram | 83.8 MB |
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| EGAF00008265552 | cram | 69.7 MB |
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| EGAF00008265554 | cram | 89.3 MB |
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| EGAF00008265556 | cram | 87.4 MB |
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| EGAF00008265563 | cram | 81.7 MB |
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| EGAF00008265949 | cram | 67.5 MB |
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| EGAF00008265950 | cram | 73.5 MB |
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| EGAF00008265952 | cram | 77.1 MB |
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| EGAF00008265954 | cram | 65.9 MB |
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| EGAF00008265958 | cram | 81.3 MB |
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| EGAF00008265961 | cram | 67.9 MB |
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| EGAF00008265963 | cram | 86.4 MB |
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| EGAF00008265965 | cram | 84.7 MB |
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| EGAF00008265972 | cram | 79.1 MB |
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| EGAF00008265974 | cram | 68.5 MB |
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| EGAF00008265975 | cram | 74.6 MB |
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| EGAF00008265977 | cram | 78.3 MB |
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| EGAF00008265979 | cram | 66.7 MB |
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| EGAF00008265983 | cram | 82.4 MB |
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| EGAF00008265986 | cram | 68.4 MB |
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| EGAF00008265988 | cram | 87.7 MB |
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| EGAF00008265990 | cram | 86.2 MB |
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| EGAF00008265997 | cram | 80.3 MB |
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| EGAF00008295010 | cram | 81.5 MB |
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| EGAF00008295012 | cram | 90.5 MB |
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| EGAF00008295015 | cram | 85.6 MB |
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| EGAF00008295018 | cram | 82.5 MB |
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| EGAF00008295020 | cram | 98.9 MB |
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| EGAF00008295024 | cram | 84.3 MB |
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| EGAF00008295025 | cram | 72.2 MB |
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| EGAF00008295028 | cram | 86.9 MB |
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| EGAF00008295034 | cram | 84.6 MB |
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| EGAF00008295036 | cram | 93.7 MB |
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| EGAF00008295039 | cram | 88.9 MB |
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| EGAF00008295042 | cram | 84.9 MB |
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| EGAF00008295044 | cram | 102.7 MB |
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| EGAF00008295048 | cram | 87.7 MB |
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| EGAF00008295049 | cram | 74.6 MB |
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| EGAF00008295052 | cram | 90.3 MB |
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| EGAF00008295058 | cram | 84.4 MB |
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| EGAF00008295060 | cram | 93.2 MB |
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| EGAF00008295063 | cram | 88.7 MB |
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| EGAF00008295066 | cram | 84.5 MB |
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| EGAF00008295068 | cram | 102.3 MB |
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| EGAF00008295072 | cram | 87.4 MB |
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| EGAF00008295073 | cram | 74.5 MB |
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| EGAF00008295076 | cram | 89.7 MB |
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| EGAF00008295082 | cram | 80.5 MB |
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| EGAF00008295084 | cram | 89.0 MB |
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| EGAF00008295087 | cram | 84.5 MB |
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| EGAF00008295090 | cram | 81.0 MB |
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| EGAF00008295092 | cram | 97.8 MB |
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| EGAF00008295096 | cram | 83.3 MB |
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| EGAF00008295097 | cram | 71.3 MB |
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| EGAF00008295100 | cram | 85.6 MB |
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| EGAF00008610387 | cram | 502.9 MB |
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| EGAF00008610388 | cram | 468.3 MB |
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| EGAF00008610389 | cram | 468.5 MB |
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| EGAF00008610390 | cram | 385.3 MB |
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| EGAF00008610393 | cram | 386.6 MB |
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| EGAF00008610394 | cram | 444.7 MB |
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| EGAF00008610396 | cram | 463.8 MB |
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| EGAF00008610397 | cram | 387.3 MB |
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| EGAF00009003868 | cram | 15.6 GB |
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| EGAF00009003877 | cram | 15.7 GB |
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| EGAF00009003889 | cram | 12.4 GB |
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| EGAF00009003892 | cram | 11.0 GB |
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| EGAF00009003900 | cram | 12.5 GB |
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| EGAF00009003903 | cram | 11.1 GB |
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| EGAF00009003905 | cram | 16.1 GB |
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| EGAF00009003910 | cram | 18.5 GB |
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| EGAF00009003914 | cram | 16.1 GB |
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| EGAF00009003919 | cram | 18.6 GB |
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| EGAF00009003957 | cram | 24.7 GB |
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| EGAF00009004137 | cram | 14.2 GB |
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| EGAF00009004141 | cram | 13.2 GB |
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| EGAF00009004143 | cram | 12.9 GB |
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| EGAF00009004147 | cram | 14.1 GB |
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| EGAF00009004149 | cram | 13.2 GB |
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| EGAF00009004151 | cram | 12.9 GB |
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| EGAF00009004171 | cram | 1.1 GB |
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| EGAF00009004175 | cram | 14.7 GB |
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| EGAF00009004177 | cram | 12.3 GB |
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| EGAF00009004180 | cram | 15.6 GB |
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| EGAF00009004181 | cram | 15.3 GB |
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| EGAF00009004182 | cram | 12.0 GB |
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| EGAF00009004183 | cram | 15.1 GB |
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| EGAF00009004186 | cram | 12.5 GB |
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| EGAF00009004189 | cram | 16.0 GB |
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| EGAF00009004190 | cram | 15.5 GB |
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| EGAF00009004191 | cram | 12.4 GB |
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| EGAF00009004192 | cram | 31.5 GB |
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| EGAF00009004194 | cram | 26.6 GB |
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| EGAF00009004195 | cram | 32.2 GB |
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| EGAF00009004197 | cram | 1.7 GB |
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| EGAF00009004198 | cram | 26.1 GB |
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| EGAF00009004199 | cram | 21.1 GB |
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| EGAF00009004200 | cram | 24.1 GB |
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| EGAF00009004203 | cram | 24.7 GB |
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| 145 Files (611.5 GB) | ||||