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RNA-seq data for study 'Smoking-dependent expression alterations in nasal epithelium reveal immune impairment linked to germline variation and lung cancer risk.'

RNA-seq data from nasal and bronchial tissues in 649 subjects, many with lung cancer. Lung cancer is the leading cause of cancer-related death in the world. In contrast to many other cancers, a direct connection to lifestyle risk in the form of cigarette smoke has long been established. More than 50% of all smoking-related lung cancers occur in former smokers, often many years after smoking cessation. Despite extensive research, the molecular processes for persistent lung cancer risk are unclear. CT screening of current and former smokers has been shown to reduce lung cancer mortality by up to 26%. To examine whether clinical risk stratification can be improved upon by the addition of genetic data, and to explore the mechanisms of the persisting risk in former smokers, we have analyzed transcriptomic data from accessible airway tissues of 487 subjects. We developed a model to assess smoking associated gene expression changes and their reversibility after smoking is stopped, in both healthy subjects and clinic patients. We find persistent smoking associated immune alterations to be a hallmark of the clinic patients. Integrating previous GWAS data using a transcriptional network approach, we demonstrate that the same immune and interferon related pathways are strongly enriched for genes linked to known genetic risk factors, demonstrating a causal relationship between immune alteration and lung cancer risk. Finally, we used accessible airway transcriptomic data to derive a non-invasive lung cancer risk classifier. Our results provide initial evidence for germline-mediated personalised smoke injury response and risk in the general population, with potential implications for managing long-term lung cancer incidence and mortality.

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Data Access Agreement for managed access to data for the study: 'Smoking-dependent expression alterations in nasal epithelium reveal immune impairment linked to germline variation and lung cancer risk'

The following data access agreement will be sent to requestors who have made a request through the EGA archive. DATA ACCESS AGREEMENT These terms and conditions govern access to the managed access datasets (details of which are set out in Appendix I) to which the User Institution has requested access. The User Institution agrees to be bound by these terms and conditions. Definitions Authorised Personnel: The individuals at the User Institution to whom the University of Cambridge grants access to the Data. This includes the User, the individuals listed in Appendix II and any other individuals for whom the User Institution subsequently requests access to the Data. Details of the initial Authorised Personnel are set out in Appendix II. Data: The managed access datasets to which the User Institution has requested access. Data Producers: The University of Cambridge and the collaborators listed in Appendix I responsible for the development, organisation, and oversight of these Data. External Collaborator: A collaborator of the User, working for an institution other than the User Institution. Project: The project for which the User Institution has requested access to these Data. A description of the Project is set out in Appendix II. Publications: Includes, without limitation, articles published in print journals, electronic journals, reviews, books, posters and other written and verbal presentations of research. Research Participant: An individual whose data form part of these Data. Research Purposes: Shall mean research that is seeking to advance the understanding of genetics and genomics, including the treatment of disorders, and work on statistical methods that may be applied to such research. User: The principal investigator for the Project. User Institution(s): The Institution that has requested access to the Data. The Chancellor, Masters and Scholars of the University of Cambridge; of The Old Schools, Trinity Lane, Cambridge CB2 1TN 1. The User Institution agrees to only use these Data for the purpose of the Project (described in Appendix II) and only for Research Purposes. The User Institution further agrees that it will only use these Data for Research Purposes which are within the limitations (if any) set out in Appendix I. 2. The User Institution agrees to preserve, at all times, the confidentiality of these Data. In particular, it undertakes not to use, or attempt to use these Data to compromise or otherwise infringe the confidentiality of information on Research Participants. Without prejudice to the generality of the foregoing, the User Institution agrees to use at least the measures set out in Appendix I to protect these Data. 3. The User Institution agrees to protect the confidentiality of Research Participants in any research papers or publications that they prepare by taking all reasonable care to limit the possibility of identification. 4. The User Institution agrees not to link or combine these Data to other information or archived data available in a way that could re-identify the Research Participants, even if access to that data has been formally granted to the User Institution or is freely available without restriction. 5. The User Institution agrees only to transfer or disclose these Data, in whole or part, or any material derived from these Data, to the Authorised Personnel. Should the User Institution wish to share these Data with an External Collaborator, the External Collaborator must complete a separate application for access to these Data. 6. The User Institution agrees that the Data Producers, and all other parties involved in the creation, funding or protection of these Data: a) make no warranty or representation, express or implied as to the accuracy, quality or comprehensiveness of these Data; b) exclude to the fullest extent permitted by law all liability for actions, claims, proceedings, demands, losses (including but not limited to loss of profit), costs, awards damages and payments made by the Recipient that may arise (whether directly or indirectly) in any way whatsoever from the Recipient’s use of these Data or from the unavailability of, or break in access to, these Data for whatever reason and; c) bear no responsibility for the further analysis or interpretation of these Data. 7. The User Institution agrees to follow the Fort Lauderdale Guidelines (http://www.wellcome.ac.uk/stellent/groups/corporatesite/@policy_communications/documents/web_document/wtd003207.pdf ) and the Toronto Statement (http://www.nature.com/nature/journal/v461/n7261/full/461168a.html). This includes but is not limited to recognising the contribution of the Data Producers and including a proper acknowledgement in all reports or publications resulting from the use of these Data. 8. The User Institution agrees to follow the Publication Policy in Appendix III. This includes respecting the moratorium period for the Data Producers to publish the first peer-reviewed report describing and analysing these Data. 9. The User Institution agrees not to make intellectual property claims on these Data and not to use intellectual property protection in ways that would prevent or block access to, or use of, any element of these Data, or conclusion drawn directly from these Data. 10. The User Institution can elect to perform further research that would add intellectual and resource capital to these data and decide to obtain intellectual property rights on these downstream discoveries. In this case, the User Institution agrees to implement licensing policies that will not obstruct further research and to follow the U.S. National Institutes of Health Best Practices for the Licensing of Genomic Inventions (2005) (https://www.icgc.org/files/daco/NIH_BestPracticesLicensingGenomicInventions_2005_en.pdf ) in conformity with the Organisation for Economic Co-operation and Development Guidelines for the Licensing of the Genetic Inventions (2006) (http://www.oecd.org/science/biotech/36198812.pdf ). 11. The User Institution agrees to destroy/discard the Data held, once it is no longer used for the Project, unless obliged to retain the data for archival purposes in conformity with audit or legal requirements. 12. The User Institution will notify The University of Cambridge within 30 days of any changes or departures of Authorised Personnel. 13. The User Institution will notify The University of Cambridge prior to any significant changes to the protocol for the Project. 14. The User Institution will notify The University of Cambridge as soon as it becomes aware of a breach of the terms or conditions of this agreement. 15. The University of Cambridge may terminate this agreement by written notice to the User Institution. If this agreement terminates for any reason, the User Institution will be required to destroy any Data held, including copies and backup copies. This clause does not prevent the User Institution from retaining these data for archival purpose in conformity with audit or legal requirements. 16. The User Institution accepts that it may be necessary for the Data Producers to alter the terms of this agreement from time to time. As an example, this may include specific provisions relating to the Data required by Data Producers other than Cancer Research UK Cambridge Institute. In the event that changes are required, the Data Producers or their appointed agent will contact the User Institution to inform it of the changes and the User Institution may elect to accept the changes or terminate the agreement. 17. If requested, the User Institution will allow data security and management documentation to be inspected to verify that it is complying with the terms of this agreement. 18. The User Institution agrees to distribute a copy of these terms to the Authorised Personnel. The User Institution will procure that the Authorised Personnel comply with the terms of this agreement. 19. This agreement (and any dispute, controversy, proceedings or claim of whatever nature arising out of this agreement or its formation) shall be construed, interpreted and governed by the laws of England and Wales and shall be subject to the exclusive jurisdiction of the English courts. Agreed for User Institution Signature: Name: Title: Date: Principal Investigator I confirm that I have read and understood this Agreement. Signature: Name: Title: Date: Agreed for Cancer Research UK Cambridge Institute Signature: Name: Title: Date: APPENDIX I – DATASET DETAILS APPENDIX II ––PROJECT DETAILS APPENDIX III –– PUBLICATION POLICY APPENDIX I – DATASET DETAILS (to be completed by the data producer before passing to applicant) Dataset reference EGA study ID: Project details Lung cancer is the leading cause of cancer-related death in the world. In contrast to many other cancers, a direct connection to lifestyle risk in the form of cigarette smoke has long been established. More than 50% of all smoking-related lung cancers occur in former smokers, often many years after smoking cessation. Despite extensive research, the molecular processes for persistent lung cancer risk are unclear. CT screening of current and former smokers has been shown to reduce lung cancer mortality by up to 26%. To examine whether clinical risk stratification can be improved upon by the addition of genetic data, and to explore the mechanisms of the persisting risk in former smokers, we have analyzed transcriptomic data from accessible airway tissues of 487 subjects. We developed a model to assess smoking associated gene expression changes and their reversibility after smoking is stopped, in both healthy subjects and clinic patients. We find persistent smoking associated immune alterations to be a hallmark of the clinic patients. Integrating previous GWAS data using a transcriptional network approach, we demonstrate that the same immune and interferon related pathways are strongly enriched for genes linked to known genetic risk factors, demonstrating a causal relationship between immune alteration and lung cancer risk. Finally, we used accessible airway transcriptomic data to derive a non-invasive lung cancer risk classifier. Our results provide initial evidence for germline-mediated personalised smoke injury response and risk in the general population, with potential implications for managing long-term lung cancer incidence and mortality. Name of project that created the dataset: Cancer Research UK Cambridge Institute: lung cancer airway risk Names of data producers/collaborators: Data producers at Cancer Research UK Cambridge Institute: Professor Robert C Rintoul Professor Bruce AJ Ponder Dr Florian Markowetz Other Data producer collaborators: Dr Roland Schwarz - Center for Integrated Oncology (CIO), Cancer Research Center Cologne Essen (CCCE), Faculty of Medicine and University Hospital Cologne, University of Cologne, Germany Dr Florian Massip - MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, 75006 Paris, France Specific limitations on areas of research: Minimum protection measures required: File access: Data can be held in unencrypted files on an institutional compute system, with Unix user group read only access for one or more appropriate groups but not Unix world read or write access behind a secure firewall. Laptops holding these data should have password protected logins and screenlocks (set to lock after 5 min of inactivity). If held on USB keys or other portable hard drives, the data must be encrypted. APPENDIX II – PROJECT DETAILS (to be completed by the Requestor) Details of dataset requested i.e., EGA Study and Dataset Accession Number Dataset Accession Number: Brief abstract of the Project in which the Data will be used (500 words max): All Individuals who the User Institution wishes to have named as registered users: Name of Registered User Email Job Title Supervisor* All Individuals that should have an account created at the EGA Name of Registered User Email Job Title APPENDIX III – PUBLICATION POLICY The University of Cambridge intend to publish the results of their analysis of this dataset and do not consider its deposition into public databases to be the equivalent of such publications. The University of Cambridge anticipate that the dataset could be useful to other qualified researchers for a variety of purposes. However, some areas of work are subject to a publication moratorium. The publication moratorium covers any publications (including oral communications) that describe the use of the dataset. For research papers, submission for publication should not occur until 12 months after these data were first made available on the relevant hosting database, unless The University of Cambridge has provided written consent to earlier submission. In any publications based on these data, please describe how the data can be accessed, including the name of the hosting database (e.g., The European Genome-phenome Archive at the European Bioinformatics Institute) and its accession numbers (e.g., EGAS00000000029), and acknowledge its use in a form agreed by the User Institution with the University of Cambridge .

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001006137 Transcriptome Analysis

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00008266774 fq.gz 93.5 MB
EGAF00008266775 fq.gz 120.5 MB
EGAF00008266776 fq.gz 118.6 MB
EGAF00008266777 fq.gz 105.3 MB
EGAF00008266778 fq.gz 119.5 MB
EGAF00008266779 fq.gz 116.6 MB
EGAF00008266780 fq.gz 121.9 MB
EGAF00008266781 fq.gz 119.5 MB
EGAF00008266782 fq.gz 119.4 MB
EGAF00008266783 fq.gz 118.6 MB
EGAF00008266784 fq.gz 93.4 MB
EGAF00008266785 fq.gz 118.8 MB
EGAF00008266786 fq.gz 116.9 MB
EGAF00008266787 fq.gz 104.2 MB
EGAF00008266788 fq.gz 117.9 MB
EGAF00008266789 fq.gz 115.3 MB
EGAF00008266790 fq.gz 120.3 MB
EGAF00008266791 fq.gz 117.8 MB
EGAF00008266792 fq.gz 117.6 MB
EGAF00008266793 fq.gz 117.1 MB
EGAF00008266794 fq.gz 91.8 MB
EGAF00008266795 fq.gz 117.0 MB
EGAF00008266796 fq.gz 115.1 MB
EGAF00008266797 fq.gz 102.5 MB
EGAF00008266798 fq.gz 116.1 MB
EGAF00008266799 fq.gz 113.5 MB
EGAF00008266800 fq.gz 118.2 MB
EGAF00008266801 fq.gz 115.9 MB
EGAF00008266802 fq.gz 115.5 MB
EGAF00008266803 fq.gz 115.1 MB
EGAF00008266804 fq.gz 92.1 MB
EGAF00008266805 fq.gz 115.2 MB
EGAF00008266806 fq.gz 113.8 MB
EGAF00008266807 fq.gz 101.5 MB
EGAF00008266808 fq.gz 114.5 MB
EGAF00008266809 fq.gz 111.9 MB
EGAF00008266810 fq.gz 117.3 MB
EGAF00008266811 fq.gz 115.1 MB
EGAF00008266812 fq.gz 114.5 MB
EGAF00008266813 fq.gz 113.9 MB
EGAF00008266814 fq.gz 95.5 MB
EGAF00008266815 fq.gz 123.3 MB
EGAF00008266816 fq.gz 120.6 MB
EGAF00008266817 fq.gz 108.9 MB
EGAF00008266818 fq.gz 124.1 MB
EGAF00008266819 fq.gz 121.1 MB
EGAF00008266820 fq.gz 124.1 MB
EGAF00008266821 fq.gz 122.7 MB
EGAF00008266822 fq.gz 122.9 MB
EGAF00008266823 fq.gz 121.7 MB
EGAF00008266824 fq.gz 94.6 MB
EGAF00008266825 fq.gz 121.5 MB
EGAF00008266826 fq.gz 119.4 MB
EGAF00008266827 fq.gz 106.4 MB
EGAF00008266828 fq.gz 120.2 MB
EGAF00008266829 fq.gz 117.7 MB
EGAF00008266830 fq.gz 122.4 MB
EGAF00008266831 fq.gz 120.3 MB
EGAF00008266832 fq.gz 119.8 MB
EGAF00008266833 fq.gz 119.3 MB
EGAF00008266834 fq.gz 98.7 MB
EGAF00008266835 fq.gz 126.0 MB
EGAF00008266836 fq.gz 123.8 MB
EGAF00008266837 fq.gz 110.4 MB
EGAF00008266838 fq.gz 125.2 MB
EGAF00008266839 fq.gz 122.2 MB
EGAF00008266840 fq.gz 127.7 MB
EGAF00008266841 fq.gz 125.4 MB
EGAF00008266842 fq.gz 124.8 MB
EGAF00008266843 fq.gz 124.0 MB
EGAF00008266844 fq.gz 92.6 MB
EGAF00008266845 fq.gz 118.2 MB
EGAF00008266846 fq.gz 116.3 MB
EGAF00008266847 fq.gz 103.3 MB
EGAF00008266848 fq.gz 116.9 MB
EGAF00008266849 fq.gz 114.4 MB
EGAF00008266850 fq.gz 119.4 MB
EGAF00008266851 fq.gz 117.5 MB
EGAF00008266852 fq.gz 116.7 MB
EGAF00008266853 fq.gz 116.1 MB
EGAF00008266854 fq.gz 85.8 MB
EGAF00008266855 fq.gz 110.3 MB
EGAF00008266856 fq.gz 108.7 MB
EGAF00008266857 fq.gz 96.9 MB
EGAF00008266858 fq.gz 110.1 MB
EGAF00008266859 fq.gz 107.9 MB
EGAF00008266860 fq.gz 111.8 MB
EGAF00008266861 fq.gz 110.7 MB
EGAF00008266862 fq.gz 110.0 MB
EGAF00008266863 fq.gz 109.0 MB
EGAF00008266864 fq.gz 78.3 MB
EGAF00008266865 fq.gz 99.2 MB
EGAF00008266866 fq.gz 97.9 MB
EGAF00008266867 fq.gz 87.0 MB
EGAF00008266868 fq.gz 98.3 MB
EGAF00008266869 fq.gz 96.1 MB
EGAF00008266870 fq.gz 100.4 MB
EGAF00008266871 fq.gz 98.7 MB
EGAF00008266872 fq.gz 97.9 MB
EGAF00008266873 fq.gz 97.7 MB
EGAF00008266874 fq.gz 78.0 MB
EGAF00008266875 fq.gz 98.6 MB
EGAF00008266876 fq.gz 97.4 MB
EGAF00008266877 fq.gz 87.0 MB
EGAF00008266878 fq.gz 98.5 MB
EGAF00008266879 fq.gz 96.0 MB
EGAF00008266880 fq.gz 100.4 MB
EGAF00008266881 fq.gz 99.1 MB
EGAF00008266882 fq.gz 98.3 MB
EGAF00008266883 fq.gz 97.5 MB
EGAF00008266884 fq.gz 93.6 MB
EGAF00008266885 fq.gz 120.4 MB
EGAF00008266886 fq.gz 118.1 MB
EGAF00008266887 fq.gz 105.8 MB
EGAF00008266888 fq.gz 120.6 MB
EGAF00008266889 fq.gz 117.8 MB
EGAF00008266890 fq.gz 121.5 MB
EGAF00008266891 fq.gz 119.9 MB
EGAF00008266892 fq.gz 119.5 MB
EGAF00008266893 fq.gz 118.4 MB
EGAF00008266894 fq.gz 82.0 MB
EGAF00008266895 fq.gz 105.7 MB
EGAF00008266896 fq.gz 103.9 MB
EGAF00008266897 fq.gz 92.2 MB
EGAF00008266898 fq.gz 104.4 MB
EGAF00008266899 fq.gz 102.1 MB
EGAF00008266900 fq.gz 106.2 MB
EGAF00008266901 fq.gz 104.6 MB
EGAF00008266902 fq.gz 104.0 MB
EGAF00008266903 fq.gz 103.6 MB
EGAF00008266904 fq.gz 75.7 MB
EGAF00008266905 fq.gz 97.9 MB
EGAF00008266906 fq.gz 96.1 MB
EGAF00008266907 fq.gz 85.8 MB
EGAF00008266908 fq.gz 97.2 MB
EGAF00008266909 fq.gz 95.3 MB
EGAF00008266910 fq.gz 98.4 MB
EGAF00008266911 fq.gz 97.0 MB
EGAF00008266912 fq.gz 96.6 MB
EGAF00008266913 fq.gz 96.0 MB
EGAF00008266914 fq.gz 71.1 MB
EGAF00008266915 fq.gz 91.6 MB
EGAF00008266916 fq.gz 89.6 MB
EGAF00008266917 fq.gz 81.0 MB
EGAF00008266918 fq.gz 91.9 MB
EGAF00008266919 fq.gz 90.0 MB
EGAF00008266920 fq.gz 92.4 MB
EGAF00008266921 fq.gz 91.3 MB
EGAF00008266922 fq.gz 91.2 MB
EGAF00008266923 fq.gz 90.2 MB
EGAF00008266924 fq.gz 83.4 MB
EGAF00008266925 fq.gz 107.3 MB
EGAF00008266926 fq.gz 105.2 MB
EGAF00008266927 fq.gz 94.1 MB
EGAF00008266928 fq.gz 106.6 MB
EGAF00008266929 fq.gz 104.3 MB
EGAF00008266930 fq.gz 107.9 MB
EGAF00008266931 fq.gz 106.2 MB
EGAF00008266932 fq.gz 105.8 MB
EGAF00008266933 fq.gz 105.3 MB
EGAF00008266934 fq.gz 86.1 MB
EGAF00008266935 fq.gz 110.5 MB
EGAF00008266936 fq.gz 108.5 MB
EGAF00008266937 fq.gz 97.6 MB
EGAF00008266938 fq.gz 110.4 MB
EGAF00008266939 fq.gz 108.0 MB
EGAF00008266940 fq.gz 111.9 MB
EGAF00008266941 fq.gz 110.3 MB
EGAF00008266942 fq.gz 109.8 MB
EGAF00008266943 fq.gz 108.8 MB
EGAF00008266944 fq.gz 68.2 MB
EGAF00008266945 fq.gz 87.4 MB
EGAF00008266946 fq.gz 85.5 MB
EGAF00008266947 fq.gz 76.7 MB
EGAF00008266948 fq.gz 86.8 MB
EGAF00008266949 fq.gz 85.5 MB
EGAF00008266950 fq.gz 87.9 MB
EGAF00008266951 fq.gz 86.6 MB
EGAF00008266952 fq.gz 86.0 MB
EGAF00008266953 fq.gz 85.9 MB
EGAF00008266954 fq.gz 75.4 MB
EGAF00008266955 fq.gz 97.2 MB
EGAF00008266956 fq.gz 95.1 MB
EGAF00008266957 fq.gz 85.0 MB
EGAF00008266958 fq.gz 95.9 MB
EGAF00008266959 fq.gz 94.2 MB
EGAF00008266960 fq.gz 97.2 MB
EGAF00008266961 fq.gz 95.5 MB
EGAF00008266962 fq.gz 95.3 MB
EGAF00008266963 fq.gz 95.0 MB
EGAF00008266964 fq.gz 80.0 MB
EGAF00008266965 fq.gz 102.5 MB
EGAF00008266966 fq.gz 100.7 MB
EGAF00008266967 fq.gz 89.8 MB
EGAF00008266968 fq.gz 101.9 MB
EGAF00008266969 fq.gz 99.8 MB
EGAF00008266970 fq.gz 103.8 MB
EGAF00008266971 fq.gz 102.0 MB
EGAF00008266972 fq.gz 101.5 MB
EGAF00008266973 fq.gz 100.9 MB
EGAF00008266974 fq.gz 91.4 MB
EGAF00008266975 fq.gz 118.0 MB
EGAF00008266976 fq.gz 115.7 MB
EGAF00008266977 fq.gz 103.5 MB
EGAF00008266978 fq.gz 117.5 MB
EGAF00008266979 fq.gz 114.8 MB
EGAF00008266980 fq.gz 119.2 MB
EGAF00008266981 fq.gz 117.7 MB
EGAF00008266982 fq.gz 117.1 MB
EGAF00008266983 fq.gz 116.0 MB
EGAF00008266984 fq.gz 83.5 MB
EGAF00008266985 fq.gz 108.1 MB
EGAF00008266986 fq.gz 106.3 MB
EGAF00008266987 fq.gz 94.3 MB
EGAF00008266988 fq.gz 107.0 MB
EGAF00008266989 fq.gz 105.0 MB
EGAF00008266990 fq.gz 108.9 MB
EGAF00008266991 fq.gz 107.2 MB
EGAF00008266992 fq.gz 106.8 MB
EGAF00008266993 fq.gz 106.2 MB
EGAF00008266994 fq.gz 84.4 MB
EGAF00008266995 fq.gz 108.1 MB
EGAF00008266996 fq.gz 106.1 MB
EGAF00008266997 fq.gz 94.8 MB
EGAF00008266998 fq.gz 107.1 MB
EGAF00008266999 fq.gz 105.0 MB
EGAF00008267000 fq.gz 109.0 MB
EGAF00008267001 fq.gz 107.0 MB
EGAF00008267002 fq.gz 106.5 MB
EGAF00008267003 fq.gz 106.2 MB
EGAF00008267004 fq.gz 90.4 MB
EGAF00008267005 fq.gz 116.0 MB
EGAF00008267006 fq.gz 113.9 MB
EGAF00008267007 fq.gz 101.4 MB
EGAF00008267008 fq.gz 115.0 MB
EGAF00008267009 fq.gz 112.7 MB
EGAF00008267010 fq.gz 116.7 MB
EGAF00008267011 fq.gz 114.8 MB
EGAF00008267012 fq.gz 114.3 MB
EGAF00008267013 fq.gz 113.8 MB
EGAF00008267014 fq.gz 82.2 MB
EGAF00008267015 fq.gz 104.7 MB
EGAF00008267016 fq.gz 102.7 MB
EGAF00008267017 fq.gz 92.0 MB
EGAF00008267018 fq.gz 103.9 MB
EGAF00008267019 fq.gz 101.6 MB
EGAF00008267020 fq.gz 106.0 MB
EGAF00008267021 fq.gz 104.3 MB
EGAF00008267022 fq.gz 103.7 MB
EGAF00008267023 fq.gz 103.0 MB
EGAF00008267024 fq.gz 88.7 MB
EGAF00008267025 fq.gz 114.1 MB
EGAF00008267026 fq.gz 111.6 MB
EGAF00008267027 fq.gz 99.9 MB
EGAF00008267028 fq.gz 113.1 MB
EGAF00008267029 fq.gz 111.0 MB
EGAF00008267030 fq.gz 114.7 MB
EGAF00008267031 fq.gz 112.9 MB
EGAF00008267032 fq.gz 112.3 MB
EGAF00008267033 fq.gz 111.8 MB
EGAF00008267034 fq.gz 89.7 MB
EGAF00008267035 fq.gz 116.0 MB
EGAF00008267036 fq.gz 113.6 MB
EGAF00008267037 fq.gz 101.2 MB
EGAF00008267038 fq.gz 114.3 MB
EGAF00008267039 fq.gz 112.1 MB
EGAF00008267040 fq.gz 116.1 MB
EGAF00008267041 fq.gz 114.2 MB
EGAF00008267042 fq.gz 113.5 MB
EGAF00008267043 fq.gz 113.2 MB
EGAF00008267044 fq.gz 84.9 MB
EGAF00008267045 fq.gz 109.0 MB
EGAF00008267046 fq.gz 107.1 MB
EGAF00008267047 fq.gz 95.6 MB
EGAF00008267048 fq.gz 108.4 MB
EGAF00008267049 fq.gz 106.0 MB
EGAF00008267050 fq.gz 110.1 MB
EGAF00008267051 fq.gz 108.3 MB
EGAF00008267052 fq.gz 107.8 MB
EGAF00008267053 fq.gz 107.3 MB
EGAF00008267054 fq.gz 77.9 MB
EGAF00008267055 fq.gz 99.7 MB
EGAF00008267056 fq.gz 97.8 MB
EGAF00008267057 fq.gz 87.3 MB
EGAF00008267058 fq.gz 98.9 MB
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4322 Files (446.5 GB)