Ribosomal profiling on Epstein-Barr virus transformed B-lymphoblastoid cell lines.
This dataset contains Ribo-seq BAM files of Epstein-Barr virus-transformed B-lymphoblastoid cell lines from six individuals (HG00114, HG00282, NA12005, NA12044, NA12717 and NA12751) of the GEUVADIS Project. For each EBV-LCL, 20 million cells were treated with 2 μg/ml harringtonine and 100 μg/ml cycloheximide. After lysing cells in lysis buffer supplemented with 100 μg/ml cycloheximide, ribosome complexes were purified by density purification. Small RNA molecules were isolated using the NucleoSpin miRNA Kit (Bioke, Leiden, Netherlands), followed by RNA PAGE gel separation. Universal linkers were added after dephosphorylation. After reverse transcription, the cDNA was circularized and, after ribosomal RNA depletion, barcoded and sequenced on Illumina NextSeq 500.
- 27/01/2025
- 6 samples
- DAC: EGAC50000000209
- Technology: NextSeq 500
- PUB DUO:0000019 (version: 2021-02-23)publication requiredThis data use modifier indicates that requestor agrees to make results of studies using the data available to the larger scientific community.
- IS DUO:0000028 (version: 2021-02-23)institution specific restrictionThis data use modifier indicates that use is limited to use within an approved institution.
Ribosomal profiling for EBV-LCLs
No restricted access because the study concerns cell lines that have been sequenced as parts of the 1000 Genomes Project. We will require citation plus acknowledgement. Data will be shared upon publication.
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| EGAS50000000322 | Transcriptome Sequencing |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Located in | |
|---|---|---|---|---|
| EGAF50000104223 | bam | 725.5 MB | ||
| EGAF50000104224 | bam | 1.0 GB | ||
| EGAF50000104225 | bam | 834.0 MB | ||
| EGAF50000104226 | bam | 620.6 MB | ||
| EGAF50000104227 | bam | 729.7 MB | ||
| EGAF50000104228 | bam | 1.0 GB | ||
| 6 Files (4.9 GB) | ||||