RNA sequencing from patient-derived intestinal organoids
uman duodenal tissues for establishing organoid cultures used in this study were sourced from de-identified surgical specimens (n = 3) of the duodenum obtained from patients who had undergone biopsy procedures unrelated to CeD at Tampere University Hospital. The protocol was approved by the Ethics Committee of Tampere University Hospital, Tampere, Finland (ETL code R18082). RNA from the duodenal organoids was isolated using an RNeasy Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. RNA purity and concentration were measured using a NanoDrop One spectrophotometer (NanoDrop Technologies, Wilmington, Delaware, USA). Preparation of the RNA library and transcriptome sequencing was conducted by Novogene Co., LTD (Cambridge, UK). Messenger RNA was purified from total RNA using polyA selection and subjected to library construction. Sequencing was performed on an Illumina platform, and 150 bp paired-end reads were generated.
- 20/06/2024
- 11 samples
- DAC: EGAC50000000233
- Technology: Illumina NovaSeq 6000
- NPUNCU DUO:0000018 (version: 2021-02-23)not for profit, non commercial use onlyThis data use modifier indicates that use of the data is limited to not-for-profit organizations and not-for-profit use, non-commercial use.
- COL DUO:0000020 (version: 2021-02-23)collaboration requiredThis data use modifier indicates that the requestor must agree to collaboration with the primary study investigator(s).
- IRB DUO:0000021 (version: 2021-02-23)ethics approval requiredThis data use modifier indicates that the requestor must provide documentation of local IRB/ERB approval.
- GRU DUO:0000042 (version: 2021-02-23)general research useThis data use permission indicates that use is allowed for general research use for any research purpose.
Two researchers from Tampere and one from Dr Falkpharma GmbH
Data will be shared if the proposed project falls within the patient/caretaker consent
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| EGAS50000000338 | Transcriptome Sequencing |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Located in | |
|---|---|---|---|---|
| EGAF50000108120 | fq.gz | 2.0 GB | ||
| EGAF50000108121 | fq.gz | 203.8 MB | ||
| EGAF50000108122 | fq.gz | 259.8 MB | ||
| EGAF50000108123 | fq.gz | 94.0 MB | ||
| EGAF50000108124 | fq.gz | 1.8 GB | ||
| EGAF50000108125 | fq.gz | 714.9 MB | ||
| EGAF50000108126 | fq.gz | 858.4 MB | ||
| EGAF50000108127 | fq.gz | 855.0 MB | ||
| EGAF50000108128 | fq.gz | 1.0 GB | ||
| EGAF50000108129 | fq.gz | 316.2 MB | ||
| EGAF50000108130 | fq.gz | 457.6 MB | ||
| EGAF50000108131 | fq.gz | 1.8 GB | ||
| EGAF50000108132 | fq.gz | 1.1 GB | ||
| EGAF50000108133 | fq.gz | 643.9 MB | ||
| EGAF50000108134 | fq.gz | 68.2 MB | ||
| EGAF50000108135 | fq.gz | 970.9 MB | ||
| EGAF50000108136 | fq.gz | 342.2 MB | ||
| EGAF50000108137 | fq.gz | 884.8 MB | ||
| EGAF50000108138 | fq.gz | 24.2 MB | ||
| EGAF50000108139 | fq.gz | 569.3 MB | ||
| EGAF50000108140 | fq.gz | 192.5 MB | ||
| EGAF50000108141 | fq.gz | 1.9 GB | ||
| EGAF50000108374 | txt | 2.6 MB | ||
| 23 Files (17.0 GB) | ||||