Single cell RNA sequencing data of advanced melanoma patients treated with checkpoint inhibitor immunotherapy
Single cell RNA libraries were prepared using 10x Genomics Chromium Next GEM Single Cell 3’ v2 reagents. The samples were barcoded and each library were pooled with two samples at equimolar concentrations. The pooled libraries (n=4) were sequenced on the NextSeq 500 machine (Illumina) with paired-end sequencing and dual indexing as recommended in the manufacturer’s protocol; 26 and 98 cycles for the respective Read 1 and 2, and 8 cycles for i7 index.
- 10/05/2024
- 4 samples
- DAC: EGAC00001003081
- Technology: NextSeq 500
The data sets were collected under the Melanoma Institute Australia Biospecimen Bank for Melanoma Research Protocol, Melanoma Research Database Protocol and Melanoma Institute Australia Molecular Pathology, Genomics and Clinical Outcomes Of Melanoma And Related Skin Tumor Protocol.
Samples were collected under the Melanoma Institute Australia Biospecimen Bank for Melanoma Research Protocol (X15-0454, HREC/11/RPAH/444), and clinical data were collected under the Melanoma Institute Australia: Melanoma Research Database Protocol (X15-0311, HREC/10/RPAH/530). Analysis was conducted under the Melanoma Institute Australia Molecular Pathology, Genomics and Clinical Outcomes Of Melanoma And Related Skin Tumor Protocol (X17-0312, HREC/11/RPAH/32)
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| EGAS50000000339 | RNASeq |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Located in | |
|---|---|---|---|---|
| EGAF50000108376 | fastq.gz | 42.9 GB | ||
| EGAF50000108377 | fastq.gz | 19.2 GB | ||
| EGAF50000108378 | fastq.gz | 36.8 GB | ||
| EGAF50000108379 | fastq.gz | 15.8 GB | ||
| EGAF50000108380 | fastq.gz | 16.2 GB | ||
| EGAF50000108381 | fastq.gz | 37.8 GB | ||
| EGAF50000108382 | fastq.gz | 16.3 GB | ||
| EGAF50000108383 | fastq.gz | 38.0 GB | ||
| 8 Files (223.0 GB) | ||||