WES analysis of tumor samples from ER+ breast cancer patients treated with CDK4/6 inhibitor

We performed whole exome sequencing of paired fresh frozen tumor from 4 ER+ advanced breast cancer patients. Samples were obtained for each patient prior to combined CDK4/6 inhibitor and endocrine therapy and at disease progression (N=8). DNA was extracted using the Maxwell® RSC FFPE and Tissue DNA Kit (Promega). WES was performed at the Department of Molecular Medicine, Aarhus University Hospital on matched tumor DNA (derived from primary fresh frozen and FFPE tissue) and buffy coat DNA. Libraries of tumors and matching germline DNA were prepared using 50 ng DNA and captured by Twist Comprehensive Exome with custom spike-ins, sequenced on the Illumina NovaSeq 6000 platform to an average coverage of 413x (range: 148-515x). The dataset include the analysis data files (vcf files).

15/07/2024
8 samples
DAC: EGAC00000000011

Access Policy1 Study History Files

Metadata

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TS DUO:0000025 (version: 2021-02-23)time limit on useThis data use modifier indicates that use is approved for a specific number of months.

BC Cancer, part of the Provincial Health Services Authority - Data Access Policy

Access to this data is controlled. There are a number of steps that a researcher must take to obtain access to this data, including execution of a Data Access Agreement between the institutions. The process is overseen by the Technology Development Office; please contact our general email address TDOadmin@phsa.ca. Please only click the "request data" button on the EGA website after a Data Access Agreement is fully executed.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID	Study Title	Study Type
EGAS50000000430	WES analysis of tumor samples	Exome Sequencing

updated the dataset description to match the file types uploaded (vcf instead of bam files) 15/07/2024
Dataset Released 15/07/2024

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID	File Type	Size
EGAF50000143942	vcf.gz	17.9 kB
EGAF50000143943	vcf.gz	24.4 kB
EGAF50000143944	vcf.gz	13.1 kB
EGAF50000143945	vcf.gz	14.3 kB
EGAF50000143946	vcf.gz	1.0 MB
EGAF50000143947	vcf.gz	68.2 kB
EGAF50000143948	vcf.gz	589.1 kB
EGAF50000143949	vcf.gz	44.3 kB
8 Files (1.8 MB)