Single-cell RNA sequencing of blood immune cells before and after interleukin-2 immunotherapy
Single-cell RNA sequencing of flow cytometry sorted human CD45+ immune cells from 3 systemic lupus erythematosus patients (SLE_002, SLE_004, and SLE_006) collected at baseline (day 0, before interleukin-2 immunotherpay) and day 5 (after 1 treatment cycle of interleukin-2 immunotherapy). Sorted immune cells were hash tagged and then stained with TotalSeq Human Universal Cocktail (Biolegend) before further processing for single-cell RNA sequencing using the 10X-Genomics platform. Samples from all 3 patients were pooled and loaded on 3 lanes of the chromium controller, resulting in 1 raw sequncing file for each lane.
- 13/08/2024
- 1 sample
- DAC: EGAC50000000306
- Technology: Illumina NovaSeq 6000
- NPUNCU DUO:0000018 (version: 2021-02-23)not for profit, non commercial use onlyThis data use modifier indicates that use of the data is limited to not-for-profit organizations and not-for-profit use, non-commercial use.
- GS DUO:0000022 (version: 2021-02-23)geographical restrictionThis data use modifier indicates that use is limited to within a specific geographic region.
- PS DUO:0000027 (version: 2021-02-23)project specific restrictionThis data use modifier indicates that use is limited to use within an approved project.
- GRU DUO:0000042 (version: 2021-02-23)general research useThis data use permission indicates that use is allowed for general research use for any research purpose.
Access to raw sequencing files from human participants
Sequencing files from healthy human participants and patients, collected under ethical approvals 2016-01440 and 2017-00886, have restricted access. These files are only available to researchers from countries that comply with Swiss human data confidentiality and security regulations. Furthermore, requesting researchers must adhere to the conditions outlined in the corresponding ethical approvals. These conditions include strict confidentiality requirements and a prohibition on further distribution of the data. Access to these files is governed by a Data Transfer Agreement (DTA) between the relevant institutions, which must be executed prior to the transfer of any data. The DTA will detail all terms and conditions of data usage, ensuring compliance with ethical and legal standards. This process ensures the protection of sensitive information while facilitating research collaboration.
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| EGAS50000000458 | RNASeq |
- As the article was published on August 12, 2024, the corresponding Pubmed ID was added to the corresponding studies. No other changes were implemented.
- Dataset Released
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Located in | |
|---|---|---|---|---|
| EGAF50000158245 | tar.gz | 38.5 GB | ||
| EGAF50000158246 | tar.gz | 85.0 GB | ||
| EGAF50000158294 | tar.gz | 93.2 GB | ||
| 3 Files (216.7 GB) | ||||