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Encompassing view of spatial and single-cell RNA-seq renews the role of the microvasculature in human atherosclerosis

Atherosclerosis is a pervasive contributor to ischemic heart disease and stroke. Despite the advance of lipid lowering-therapies and antihypertensive agents, the residual risk of an atherosclerotic event remains high and developing therapeutic strategies has proven challenging. This is due to the complexity of atherosclerosis with a spatial interplay of multiple cell types within the vascular wall. Here we generate an integrative high-resolution map of human atherosclerotic plaques combining single-cell RNA-seq from multiple studies and spatial transcriptomics data from 12 human specimens, with different stages of atherosclerosis. We show cell-type and atherosclerosis-specific expression changes and spatially constrained alterations in cell-cell communication. We highlight the possible recruitment of lymphocytes via ACKR1 endothelial cells of the vasa vasorum, the migration of vascular smooth muscle cells towards the lumen by transforming into fibromyocytes, and cell-cell communication in the plaque region, indicating an intricate cellular interplay within the adventitia and the subendothelial space in human atherosclerosis.

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Access is granted according to Danish National ethics regulation via application to the Videnskabsetisk Committee and will comply with Danish law and GDPR

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000000660 Transcriptome Sequencing

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF50000219584 fastq.gz 915.8 MB
EGAF50000219585 fastq.gz 887.1 MB
EGAF50000219586 fastq.gz 3.2 GB
EGAF50000219587 fastq.gz 2.3 GB
EGAF50000219588 fastq.gz 704.9 MB
EGAF50000219589 fastq.gz 828.5 MB
EGAF50000219590 fastq.gz 2.2 GB
EGAF50000219591 fastq.gz 3.2 GB
EGAF50000219592 fastq.gz 459.4 MB
EGAF50000219593 fastq.gz 660.1 MB
EGAF50000219594 fastq.gz 150.3 MB
EGAF50000219595 fastq.gz 184.1 MB
EGAF50000219596 fastq.gz 335.3 MB
EGAF50000219597 fastq.gz 373.7 MB
EGAF50000219598 fastq.gz 901.0 MB
EGAF50000219599 fastq.gz 1.3 GB
EGAF50000219600 fastq.gz 231.7 MB
EGAF50000219601 fastq.gz 262.6 MB
EGAF50000219602 fastq.gz 676.8 MB
EGAF50000219603 fastq.gz 991.2 MB
EGAF50000219604 fastq.gz 911.4 MB
EGAF50000219605 fastq.gz 3.2 GB
EGAF50000219606 fastq.gz 885.9 MB
EGAF50000219607 fastq.gz 2.3 GB
EGAF50000219608 fastq.gz 175.6 MB
EGAF50000219609 fastq.gz 521.9 MB
EGAF50000219610 fastq.gz 754.5 MB
EGAF50000219611 fastq.gz 205.4 MB
EGAF50000219612 fastq.gz 982.0 MB
EGAF50000219613 fastq.gz 774.2 MB
EGAF50000219614 fastq.gz 2.6 GB
EGAF50000219615 fastq.gz 1.1 GB
EGAF50000219616 fastq.gz 310.8 MB
EGAF50000219617 fastq.gz 309.0 MB
EGAF50000219618 fastq.gz 778.6 MB
EGAF50000219619 fastq.gz 3.1 GB
EGAF50000219620 fastq.gz 694.7 MB
EGAF50000219621 fastq.gz 823.4 MB
EGAF50000219622 fastq.gz 2.2 GB
EGAF50000219623 fastq.gz 327.8 MB
EGAF50000219624 fastq.gz 386.9 MB
EGAF50000219625 fastq.gz 1.5 GB
EGAF50000219626 fastq.gz 1.0 GB
EGAF50000219627 fastq.gz 174.3 MB
EGAF50000219628 fastq.gz 750.5 MB
EGAF50000219629 fastq.gz 204.3 MB
EGAF50000219630 fastq.gz 519.5 MB
EGAF50000219631 fastq.gz 1.4 GB
EGAF50000219632 fastq.gz 2.0 GB
EGAF50000219633 fastq.gz 503.6 MB
EGAF50000219634 fastq.gz 559.4 MB
EGAF50000219635 fastq.gz 377.4 MB
EGAF50000219636 fastq.gz 907.5 MB
EGAF50000219637 fastq.gz 338.5 MB
EGAF50000219638 fastq.gz 1.3 GB
EGAF50000219639 fastq.gz 205.5 MB
EGAF50000219640 fastq.gz 239.2 MB
EGAF50000219641 fastq.gz 598.2 MB
EGAF50000219642 fastq.gz 865.4 MB
EGAF50000219643 fastq.gz 311.2 MB
EGAF50000219644 fastq.gz 779.0 MB
EGAF50000219645 fastq.gz 1.1 GB
EGAF50000219646 fastq.gz 308.6 MB
EGAF50000219647 fastq.gz 1.7 GB
EGAF50000219648 fastq.gz 417.3 MB
EGAF50000219649 fastq.gz 470.6 MB
EGAF50000219650 fastq.gz 1.2 GB
EGAF50000219651 fastq.gz 1.3 GB
EGAF50000219652 fastq.gz 1.2 GB
EGAF50000219653 fastq.gz 420.0 MB
EGAF50000219654 fastq.gz 470.1 MB
EGAF50000219655 fastq.gz 505.2 MB
EGAF50000219656 fastq.gz 564.5 MB
EGAF50000219657 fastq.gz 1.4 GB
EGAF50000219658 fastq.gz 2.0 GB
EGAF50000219659 fastq.gz 206.3 MB
EGAF50000219660 fastq.gz 238.7 MB
EGAF50000219661 fastq.gz 597.4 MB
EGAF50000219662 fastq.gz 863.3 MB
EGAF50000219663 fastq.gz 995.9 MB
EGAF50000219664 fastq.gz 233.2 MB
EGAF50000219665 fastq.gz 679.1 MB
EGAF50000219666 fastq.gz 263.5 MB
EGAF50000219667 fastq.gz 2.6 GB
EGAF50000219668 fastq.gz 774.5 MB
EGAF50000219669 fastq.gz 3.9 GB
EGAF50000219670 fastq.gz 982.8 MB
EGAF50000219671 fastq.gz 332.5 MB
EGAF50000219672 fastq.gz 392.9 MB
EGAF50000219673 fastq.gz 1.0 GB
EGAF50000219674 fastq.gz 1.5 GB
EGAF50000219675 fastq.gz 150.5 MB
EGAF50000219676 fastq.gz 185.1 MB
EGAF50000219677 fastq.gz 461.3 MB
EGAF50000219678 fastq.gz 663.7 MB
95 Files (88.9 GB)