Identification of cell type differences in FOXN1 mutation carriers by scRNA-seq
scRNA-seq was performed on the BD Rhapsody platform. CD45+ lymphocytes from two FOXN1het patients and one cord blood sample as control were sorted from frozen cryopreserved samples. The samples were split and each sample was stained with 4 Sampletag antibodies before Abseq staining with the BD Immune Discovery panel and 6 additional antibodies was performed to better classify immune cell subsets. The sorted and stained populations were pooled before loading onto the BD Rhapsody cartridge. The manufacturer's protocol was followed for library construction and samples were sequenced on an Illumina NovaSeq 6000.
- 23/02/2026
- 1 sample
- DAC: EGAC50000000476
- Technology: Illumina NovaSeq 6000
DAC Bennstein
Access to data generated is made available by completing the data access agreement for review by the data access committee and will be granted to qualified investigators for appropriate use.
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| EGAS50000001199 | Transcriptome Sequencing |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Quality Report |
Located in
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| EGAF50000277548 | fastq.gz | 154.4 GB |
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| EGAF50000277549 | fastq.gz | 57.5 GB |
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| EGAF50000401408 | csv | 1.7 kB |
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| 3 Files (211.9 GB) | ||||