About file location

As the EGA is co-managed by the EMBL-EBI and CRG, files could be located in the UK and/or Spain, as indicated by their respective flags.

Alternatively, files could be located in one of the international Federated EGA nodes, as indicated next to the node's flag.
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Comparison between b2m KO and US2 expressing iPSC lines

This dataset contains bulk RNA-seq transcriptomic profiles from a human induced pluripotent stem cell (hiPSC) line and three engineered derivatives: B2M knockout, EGFP overexpression, and US2 overexpression, alongside the parental line. Total RNA integrity was assessed using the Experion RNA StdSens 1K Analysis Kit on the Bio-Rad Experion Automated Electrophoresis System prior to library preparation and sequencing. Libraries were sequenced on an Illumina NovaSeq 6000 generating ~20 million reads per sample. Raw read quality was assessed with FastQC, reads were aligned to the human reference genome hg38 (Ensembl release 109) using STAR, and alignment/library QC metrics (e.g., orientation, composition, coverage) were evaluated with Picard.

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Policy: LUMC Department Cell and Chemistry Biology, Diabetes Group

Access to this dataset is controlled by LUMC Department Cell and Chemistry Biology, Diabetes Group. Access to data will be granted to qualified investigators for appropriate use. Please contact this DAC to request data access.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000001618 RNASeq

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
i
EGAF50000588476 fastq.gz 2.3 GB
EGAF50000588477 fastq.gz 2.4 GB
EGAF50000588478 fastq.gz 2.2 GB
EGAF50000588479 fastq.gz 2.2 GB
EGAF50000588480 fastq.gz 1.9 GB
EGAF50000588481 fastq.gz 1.9 GB
EGAF50000588482 fastq.gz 2.7 GB
EGAF50000588483 fastq.gz 2.8 GB
EGAF50000588484 fastq.gz 2.4 GB
EGAF50000588485 fastq.gz 2.5 GB
EGAF50000588486 fastq.gz 2.8 GB
EGAF50000588487 fastq.gz 2.9 GB
EGAF50000588488 fastq.gz 2.5 GB
EGAF50000588489 fastq.gz 2.6 GB
EGAF50000588490 fastq.gz 2.5 GB
EGAF50000588491 fastq.gz 2.5 GB
EGAF50000588492 fastq.gz 1.6 GB
EGAF50000588493 fastq.gz 1.7 GB
EGAF50000588494 fastq.gz 1.2 GB
EGAF50000588495 fastq.gz 1.3 GB
20 Files (45.0 GB)