About file location

As the EGA is co-managed by the EMBL-EBI and CRG, files could be located in the UK and/or Spain, as indicated by their respective flags.

Alternatively, files could be located in one of the international Federated EGA nodes, as indicated next to the node's flag.
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Single-cell transcriptome analysis of B-cell development in the ABO platform

The dataset includes samples from four individual donors, pooled at each cell-harvesting time point (days 21, 28, and 35). Single-cell RNA sequencing was performed on the 10x Genomics Chromium platform using GEM-X Single Cell 5′ v3 and GEM-X Single Cell V(D)J v3 for BCR profiling. For each timepoint, paired libraries were constructed: a 5′ GEX library and a V(D)J-B library. Per reaction 350,000-400,000 cells were loaded, aiming for 60,000 cells per reaction. Pooled libraries were sequenced on an Illumina NovaSeq X sequencer. 50,000 read pairs per cell for GEX and 5,000 read pairs per cell for V(D)J were targeted.

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Policy: LUMC Department Immunology, Staal Group

Access to this dataset is controlled by LUMC Department Immunology, Staal Group. Access to data will be granted to qualified investigators for appropriate use. Please contact this DAC to request data access.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000001693 RNASeq

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
i
EGAF50000697955 fastq.gz 8.0 GB
EGAF50000697956 fastq.gz 21.3 GB
EGAF50000697957 fastq.gz 8.7 GB
EGAF50000697958 fastq.gz 8.6 GB
EGAF50000697959 fastq.gz 4.1 GB
EGAF50000697960 fastq.gz 19.1 GB
EGAF50000697961 fastq.gz 19.2 GB
EGAF50000697962 fastq.gz 21.5 GB
EGAF50000697963 fastq.gz 4.0 GB
EGAF50000697964 fastq.gz 7.9 GB
EGAF50000697965 fastq.gz 681.5 MB
EGAF50000697966 fastq.gz 1.2 GB
EGAF50000697967 fastq.gz 1.1 GB
EGAF50000697968 fastq.gz 2.6 GB
EGAF50000697969 fastq.gz 19.2 GB
EGAF50000697970 fastq.gz 8.0 GB
EGAF50000697971 fastq.gz 19.2 GB
EGAF50000697972 fastq.gz 8.0 GB
EGAF50000697973 fastq.gz 8.8 GB
EGAF50000697974 fastq.gz 21.0 GB
EGAF50000697975 fastq.gz 8.6 GB
EGAF50000697976 fastq.gz 2.6 GB
EGAF50000697977 fastq.gz 8.6 GB
EGAF50000697978 fastq.gz 8.8 GB
EGAF50000697979 fastq.gz 21.0 GB
EGAF50000697980 fastq.gz 8.8 GB
EGAF50000697981 fastq.gz 21.4 GB
EGAF50000697982 fastq.gz 20.9 GB
EGAF50000697983 fastq.gz 20.6 GB
EGAF50000697984 fastq.gz 1.7 GB
EGAF50000697985 fastq.gz 1.7 GB
EGAF50000697986 fastq.gz 4.0 GB
EGAF50000697987 fastq.gz 1.7 GB
EGAF50000697988 fastq.gz 648.3 MB
EGAF50000697989 fastq.gz 1.5 GB
EGAF50000697990 fastq.gz 2.6 GB
EGAF50000697991 fastq.gz 2.7 GB
EGAF50000697992 fastq.gz 1.2 GB
EGAF50000697993 fastq.gz 8.6 GB
EGAF50000697994 fastq.gz 20.7 GB
EGAF50000697995 fastq.gz 4.0 GB
EGAF50000697996 fastq.gz 1.7 GB
EGAF50000697997 fastq.gz 680.4 MB
EGAF50000697998 fastq.gz 1.6 GB
EGAF50000697999 fastq.gz 1.6 GB
EGAF50000698000 fastq.gz 677.8 MB
EGAF50000698001 fastq.gz 1.5 GB
EGAF50000698002 fastq.gz 1.1 GB
48 Files (393.5 GB)