About file location

As the EGA is co-managed by the EMBL-EBI and CRG, files could be located in the UK and/or Spain, as indicated by their respective flags.

Alternatively, files could be located in one of the international Federated EGA nodes, as indicated next to the node's flag.
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Single-cell RNA-seq (10x) – melanoma samples

This dataset comprises raw FASTQ files generated from single-cell RNA sequencing of metastatic melanoma lymph node samples using the 10x Genomics Chromium platform. Single-cell suspensions were prepared from tumour tissues, and libraries were constructed using 5′ or 3′ gene expression kits following the manufacturer’s protocol. Sequencing was performed on an Illumina platform to obtain high-throughput single-cell transcriptomic profiles. The dataset includes samples from patients with differing responses to immune checkpoint inhibitor therapy, enabling characterisation of tumour-resident immune cell populations and tumour–immune interactions associated with treatment response and resistance.

Request Access

DUO:0000015
version: 2021-02-23

no general methods research

This data use modifier indicates that use does not allow methods development research (e.g., development of software or algorithms).

DUO:0000018
version: 2021-02-23

not for profit, non commercial use only

This data use modifier indicates that use of the data is limited to not-for-profit organizations and not-for-profit use, non-commercial use.

DUO:0000019
version: 2021-02-23

publication required

This data use modifier indicates that requestor agrees to make results of studies using the data available to the larger scientific community.

DUO:0000020
version: 2021-02-23

collaboration required

This data use modifier indicates that the requestor must agree to collaboration with the primary study investigator(s).

DUO:0000021
version: 2021-02-23

ethics approval required

This data use modifier indicates that the requestor must provide documentation of local IRB/ERB approval.

DUO:0000042
version: 2021-02-23

general research use

This data use permission indicates that use is allowed for general research use for any research purpose.

DUO:0000044
version: 2021-02-23

population origins or ancestry research prohibited

This data use modifier indicates use for purposes of population, origin, or ancestry research is prohibited.

Controlled Access Policy for Melanoma Immunotherapy Resistance Dataset

Access to this dataset is controlled and granted by the Data Access Committee (DAC) in accordance with participant consent, institutional policies, and applicable ethical and legal frameworks. The dataset comprises genomic, transcriptomic, and associated clinical data derived from human melanoma samples, including bulk RNA sequencing, single-cell RNA sequencing, and T cell receptor sequencing data. Data will be made available to qualified researchers for bona fide scientific research purposes. Access requests must include a clear research proposal, evidence of appropriate institutional approvals (e.g. Human Research Ethics Committee approval or equivalent), and confirmation of secure data storage and handling procedures.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000001722 Transcriptome Sequencing

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
i
EGAF50000728622 fastq.gz 20.7 GB
EGAF50000728623 fastq.gz 22.4 GB
EGAF50000728624 fastq.gz 23.7 GB
EGAF50000728625 fastq.gz 25.3 GB
EGAF50000728626 fastq.gz 25.8 GB
EGAF50000728627 fastq.gz 27.8 GB
EGAF50000728628 fastq.gz 22.5 GB
EGAF50000728629 fastq.gz 24.0 GB
EGAF50000728638 fastq.gz 23.0 GB
EGAF50000728639 fastq.gz 24.8 GB
EGAF50000728640 fastq.gz 20.2 GB
EGAF50000728641 fastq.gz 21.9 GB
EGAF50000728698 fastq.gz 23.3 GB
EGAF50000728699 fastq.gz 25.5 GB
EGAF50000728700 fastq.gz 21.4 GB
EGAF50000728701 fastq.gz 23.3 GB
EGAF50000728702 fastq.gz 22.1 GB
EGAF50000728703 fastq.gz 23.6 GB
EGAF50000728704 fastq.gz 20.0 GB
EGAF50000728705 fastq.gz 21.6 GB
EGAF50000728706 fastq.gz 21.9 GB
EGAF50000728707 fastq.gz 23.5 GB
EGAF50000728708 fastq.gz 23.1 GB
EGAF50000728709 fastq.gz 24.9 GB
24 Files (556.4 GB)