Single-cell transcriptomic profiling of metastatic colorectal cancer organoids maintained in growth medium.
Single-cell RNA sequencing was performed on metastatic colorectal cancer (mCRC) organoids derived from patient-derived xenograft (PDX) models. Organoids were seeded in 12-well plates, embedded in 200 µL of Matrigel or BME diluted 1:1 with culture medium, and maintained with EGF-supplemented growth medium. After 7 days (or 11 days) of growth, organoids were harvested, dissociated, and live single cells were sorted for single-cell RNA sequencing using the 10x Genomics platform.
- 09/07/2026
- 12 samples
- DAC: EGAC50000001019
- Technology: Illumina NovaSeq 6000
- Archive: European Genome-phenome Archive (EGA)
DUO:0000007 version: 2021-02-23
disease specific research
This data use permission indicates that use is allowed provided it is related to the specified disease.
ModifiersMONDO:0005575
Quantitative Cancer Cell Biology EGA Policy
Dataset available on request.
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| EGAS50000001927 | Transcriptome Sequencing |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Quality Report |
Located in
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| EGAF50000911685 | fastq.gz | 2.1 GB |
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| EGAF50000911686 | fastq.gz | 548.1 MB |
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| EGAF50000911687 | fastq.gz | 595.7 MB |
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| EGAF50000911688 | fastq.gz | 4.9 GB |
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| EGAF50000911689 | fastq.gz | 4.8 GB |
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| EGAF50000911690 | fastq.gz | 2.1 GB |
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| EGAF50000911691 | fastq.gz | 582.8 MB |
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| EGAF50000911692 | fastq.gz | 563.1 MB |
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| EGAF50000911693 | fastq.gz | 2.4 GB |
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| EGAF50000911694 | fastq.gz | 5.1 GB |
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| EGAF50000911695 | fastq.gz | 833.4 MB |
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| EGAF50000911696 | fastq.gz | 783.8 MB |
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| EGAF50000911697 | fastq.gz | 2.4 GB |
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| EGAF50000911698 | fastq.gz | 5.4 GB |
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| EGAF50000911699 | fastq.gz | 645.4 MB |
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| EGAF50000911700 | fastq.gz | 713.1 MB |
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| EGAF50000911701 | fastq.gz | 834.8 MB |
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| EGAF50000911702 | fastq.gz | 789.0 MB |
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| EGAF50000911703 | fastq.gz | 2.4 GB |
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| EGAF50000911704 | fastq.gz | 5.1 GB |
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| EGAF50000911705 | fastq.gz | 587.9 MB |
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| EGAF50000911706 | fastq.gz | 586.0 MB |
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| EGAF50000911707 | fastq.gz | 2.1 GB |
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| EGAF50000911708 | fastq.gz | 4.9 GB |
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| EGAF50000911709 | fastq.gz | 647.0 MB |
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| EGAF50000911710 | fastq.gz | 716.3 MB |
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| EGAF50000911711 | fastq.gz | 2.4 GB |
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| EGAF50000911712 | fastq.gz | 5.4 GB |
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| EGAF50000911713 | fastq.gz | 4.2 GB |
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| EGAF50000911714 | fastq.gz | 506.7 MB |
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| EGAF50000911715 | fastq.gz | 474.0 MB |
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| EGAF50000911716 | fastq.gz | 1.9 GB |
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| EGAF50000911717 | fastq.gz | 591.0 MB |
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| EGAF50000911718 | fastq.gz | 594.3 MB |
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| EGAF50000911719 | fastq.gz | 2.2 GB |
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| EGAF50000911720 | fastq.gz | 5.0 GB |
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| EGAF50000911721 | fastq.gz | 2.1 GB |
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| EGAF50000911722 | fastq.gz | 4.8 GB |
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| EGAF50000911723 | fastq.gz | 543.5 MB |
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| EGAF50000911724 | fastq.gz | 585.7 MB |
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| EGAF50000911725 | fastq.gz | 1.9 GB |
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| EGAF50000911726 | fastq.gz | 4.3 GB |
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| EGAF50000911727 | fastq.gz | 508.4 MB |
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| EGAF50000911728 | fastq.gz | 480.7 MB |
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| EGAF50000911729 | fastq.gz | 585.2 MB |
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| EGAF50000911730 | fastq.gz | 569.4 MB |
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| EGAF50000911731 | fastq.gz | 2.1 GB |
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| EGAF50000911732 | fastq.gz | 4.8 GB |
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| 48 Files (99.8 GB) | ||||
