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Single-cell transcriptomic profiling of metastatic colorectal cancer organoids maintained in growth medium.

Single-cell RNA sequencing was performed on metastatic colorectal cancer (mCRC) organoids derived from patient-derived xenograft (PDX) models. Organoids were seeded in 12-well plates, embedded in 200 µL of Matrigel or BME diluted 1:1 with culture medium, and maintained with EGF-supplemented growth medium. After 7 days (or 11 days) of growth, organoids were harvested, dissociated, and live single cells were sorted for single-cell RNA sequencing using the 10x Genomics platform.

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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000001927 Transcriptome Sequencing

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
EGAF50000911685 fastq.gz 2.1 GB
EGAF50000911686 fastq.gz 548.1 MB
EGAF50000911687 fastq.gz 595.7 MB
EGAF50000911688 fastq.gz 4.9 GB
EGAF50000911689 fastq.gz 4.8 GB
EGAF50000911690 fastq.gz 2.1 GB
EGAF50000911691 fastq.gz 582.8 MB
EGAF50000911692 fastq.gz 563.1 MB
EGAF50000911693 fastq.gz 2.4 GB
EGAF50000911694 fastq.gz 5.1 GB
EGAF50000911695 fastq.gz 833.4 MB
EGAF50000911696 fastq.gz 783.8 MB
EGAF50000911697 fastq.gz 2.4 GB
EGAF50000911698 fastq.gz 5.4 GB
EGAF50000911699 fastq.gz 645.4 MB
EGAF50000911700 fastq.gz 713.1 MB
EGAF50000911701 fastq.gz 834.8 MB
EGAF50000911702 fastq.gz 789.0 MB
EGAF50000911703 fastq.gz 2.4 GB
EGAF50000911704 fastq.gz 5.1 GB
EGAF50000911705 fastq.gz 587.9 MB
EGAF50000911706 fastq.gz 586.0 MB
EGAF50000911707 fastq.gz 2.1 GB
EGAF50000911708 fastq.gz 4.9 GB
EGAF50000911709 fastq.gz 647.0 MB
EGAF50000911710 fastq.gz 716.3 MB
EGAF50000911711 fastq.gz 2.4 GB
EGAF50000911712 fastq.gz 5.4 GB
EGAF50000911713 fastq.gz 4.2 GB
EGAF50000911714 fastq.gz 506.7 MB
EGAF50000911715 fastq.gz 474.0 MB
EGAF50000911716 fastq.gz 1.9 GB
EGAF50000911717 fastq.gz 591.0 MB
EGAF50000911718 fastq.gz 594.3 MB
EGAF50000911719 fastq.gz 2.2 GB
EGAF50000911720 fastq.gz 5.0 GB
EGAF50000911721 fastq.gz 2.1 GB
EGAF50000911722 fastq.gz 4.8 GB
EGAF50000911723 fastq.gz 543.5 MB
EGAF50000911724 fastq.gz 585.7 MB
EGAF50000911725 fastq.gz 1.9 GB
EGAF50000911726 fastq.gz 4.3 GB
EGAF50000911727 fastq.gz 508.4 MB
EGAF50000911728 fastq.gz 480.7 MB
EGAF50000911729 fastq.gz 585.2 MB
EGAF50000911730 fastq.gz 569.4 MB
EGAF50000911731 fastq.gz 2.1 GB
EGAF50000911732 fastq.gz 4.8 GB
48 Files (99.8 GB)