scRNAseq of single cell outgrowths of HCC organoid
FASTQ files were processed using the TrailmakerTM pipeline module (v1.5.0, Parse Biosciences). Unfiltered count matrices were uploaded to Trailmaker, and background was removed by setting a minimum transcripts per cell threshold on a per sample basis (threshold range: 998 to 1799). Dead or dying cells were removed by filtering barcodes with high mitochondrial content on a per sample basis (threshold range: 12.62% to 21.1%). Outliers in the distribution of number of genes vs number of transcripts were removed by fitting a spline regression model (p-values between 0.000003 and 0.000264). Cells with a high probability of being doublets were filtered out using the scDblFinder method on a per sample basis (threshold range: 0.72763 to 0.80853). Read counts were normalized by log1p. Output files are normalized counts.
- 30/10/2026
- 8 samples
- DAC: EGAC50000000917
- Technology: Illumina NovaSeq 6000
- Archive: European Genome-phenome Archive (EGA)
Data Access Policy – Basel Liver Biopsy Biobank (University Hospital Basel / University of Basel)
Data Access Policy – Basel Liver Biopsy Biobank (University Hospital Basel / University of Basel) Access is granted only to qualified researchers affiliated with a bona fide research institution, for a clearly described research project, and subject to review and approval by the Data Access Committee "Basel Liver Biopsy Biobank". Application requirements: A completed and signed Data Access Request and Agreement, a brief project description, and the applicant’s CV (and/or relevant track record) must be provided. Permitted use: Data may be used only for the approved research project and for the approved duration. Any new purpose requires a new request and approval. Privacy and confidentiality: Data are pseudonymised. Applicants must not attempt to re-identify individuals and must protect confidentiality at all times. Data security: Data must be handled within an institutional IT environment with appropriate access controls, malware protection, and secure backup procedures. No onward transfer: Data must not be shared or transferred outside the recipient institution or to external collaborators without prior written approval; external collaborators may require a separate agreement. Publication and acknowledgement: Publications and presentations resulting from use of the data must acknowledge the data controllers as specified in the agreement. Incident reporting: Any security breach leading to disclosure must be reported without undue delay and at the latest within 72 hours. Governing law: Switzerland (Basel-City jurisdiction), as specified in the agreement.
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| EGAS50000002198 | RNASeq |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Quality Report |
Located in
i
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|---|---|---|---|---|
| EGAF50001049108 | fastq.gz | 15.8 GB |
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| EGAF50001049109 | fastq.gz | 16.0 GB |
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| EGAF50001049110 | fastq.gz | 19.6 GB |
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| EGAF50001049111 | fastq.gz | 20.0 GB |
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| EGAF50001049112 | fastq.gz | 17.6 GB |
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| EGAF50001049113 | fastq.gz | 18.0 GB |
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| EGAF50001049114 | fastq.gz | 16.3 GB |
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| EGAF50001049115 | fastq.gz | 16.6 GB |
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| EGAF50001049116 | fastq.gz | 15.3 GB |
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| EGAF50001049117 | fastq.gz | 15.4 GB |
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| EGAF50001049118 | fastq.gz | 17.0 GB |
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| EGAF50001049119 | fastq.gz | 17.3 GB |
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| EGAF50001049120 | fastq.gz | 16.7 GB |
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| EGAF50001049121 | fastq.gz | 16.9 GB |
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| EGAF50001049122 | fastq.gz | 13.9 GB |
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| EGAF50001049123 | fastq.gz | 14.2 GB |
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| EGAF50001049124 | csv.gz | 311.2 MB |
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| EGAF50001049125 | csv.gz | 190.7 MB |
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| EGAF50001049126 | csv.gz | 523.6 MB |
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| EGAF50001049127 | csv.gz | 260.1 MB |
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| EGAF50001049128 | csv.gz | 314.6 MB |
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| EGAF50001049129 | csv.gz | 90.8 MB |
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| EGAF50001049130 | csv.gz | 130.8 MB |
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| EGAF50001049131 | csv.gz | 112.6 MB |
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| EGAF50001049132 | csv.gz | 290.6 MB |
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| EGAF50001049133 | csv.gz | 233.2 MB |
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| 26 Files (269.0 GB) | ||||
