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Buffy coat dataset 3

Single-cell DNA methylation profiling (sciMET) of buffy coat nuclei from eight healthy adult donors (3 male, 5 female; seven European and one Asian ancestry), to characterize the effects of donor genetic diversity in pooled single-cell DNA methylation experiments. The dataset was generated using a pooled design: all eight donors mixed before tagmentation, 5,000 nuclei per well, donors assigned afterwards with cellsnp-lite and Vireo). The libraries followed the sciMET v2 protocol instead of the commercial kit, with modified ligation (T4 PNK/T4 ligase with PEG 8000, 1,3-propanediol, ETSSB and H10 P5 adapter) and amplification (Kapa HiFi Uracil, 15 cycles) reactions.

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Data policy of Data Access Committee of UZ Leuven

The procedure described below always applies to re-use transfer of genomic or genetic data that are in an external database. This external database can be the European Genome-phenome Archive (EGA). When a researcher receives requests for reuse of data, or when the database requires an intervention of a data access committee, the DAC will assess these requests for reuse and transfer of genomic or genetic data by third parties. Patient consent to research participation (ICF) and compatibility with new data processing should be checked. An application should be submitted at dac@uzleuven.be. So applications for re-use of genomic/genomic data go through the DAC. In the first place, DAC performs a front desk function here. An application form should be completed by the applicant. To begin with, the DAC employee then checks the ICF and its compatibility with further use. The DAC employee also ensures that a check is made with the competent services to ensure that there are no legal impediments. If i) there are possible commercial interests (commercial use of the data by the recipient or the requesting party is a commercial party), ii) the researcher does not agree, or iii) there is some ambiguity about potential risks of the data transfer (e.g. violation (GDPR) legislation, identity of recipient party, use of the data), the question is referred to the DAC advisory board.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000002212 Epigenetics

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
EGAF50001070092 fastq.gz 22.8 GB
EGAF50001070093 fastq.gz 21.9 GB
EGAF50001070094 fastq.gz 2.8 GB
EGAF50001070095 fastq.gz 2.6 GB
EGAF50001070096 fastq.gz 3.7 GB
EGAF50001070097 fastq.gz 3.4 GB
EGAF50001070098 fastq.gz 31.6 GB
EGAF50001070099 fastq.gz 30.3 GB
EGAF50001070100 fastq.gz 37.7 GB
EGAF50001070101 fastq.gz 4.6 GB
EGAF50001070102 fastq.gz 4.1 GB
EGAF50001070103 fastq.gz 36.0 GB
EGAF50001070104 fastq.gz 6.6 GB
EGAF50001070105 fastq.gz 5.9 GB
EGAF50001070106 fastq.gz 54.9 GB
EGAF50001070107 fastq.gz 52.2 GB
EGAF50001070108 fastq.gz 2.2 GB
EGAF50001070109 fastq.gz 2.2 GB
EGAF50001070110 fastq.gz 20.3 GB
EGAF50001070111 fastq.gz 19.3 GB
EGAF50001070112 fastq.gz 5.3 GB
EGAF50001070113 fastq.gz 5.2 GB
EGAF50001070114 fastq.gz 49.8 GB
EGAF50001070115 fastq.gz 47.6 GB
EGAF50001070116 fastq.gz 43.1 GB
EGAF50001070117 fastq.gz 4.8 GB
EGAF50001070118 fastq.gz 4.8 GB
EGAF50001070119 fastq.gz 45.2 GB
EGAF50001070120 fastq.gz 2.0 GB
EGAF50001070121 fastq.gz 2.0 GB
EGAF50001070122 fastq.gz 17.9 GB
EGAF50001070123 fastq.gz 17.1 GB
EGAF50001070124 fastq.gz 5.1 GB
EGAF50001070125 fastq.gz 4.8 GB
EGAF50001070126 fastq.gz 45.5 GB
EGAF50001070127 fastq.gz 43.3 GB
EGAF50001070128 fastq.gz 4.8 GB
EGAF50001070129 fastq.gz 4.5 GB
EGAF50001070130 fastq.gz 42.7 GB
EGAF50001070131 fastq.gz 40.7 GB
EGAF50001070132 fastq.gz 2.9 GB
EGAF50001070133 fastq.gz 2.8 GB
EGAF50001070134 fastq.gz 25.1 GB
EGAF50001070135 fastq.gz 23.9 GB
EGAF50001070136 fastq.gz 6.0 GB
EGAF50001070137 fastq.gz 56.3 GB
EGAF50001070138 fastq.gz 53.6 GB
EGAF50001070139 fastq.gz 5.9 GB
48 Files (978.0 GB)