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Buffy coat dataset 2

Single-cell DNA methylation profiling (sciMET) of buffy coat nuclei from eight healthy adult donors (3 male, 5 female; seven European and one Asian ancestry), to characterize the effects of donor genetic diversity in pooled single-cell DNA methylation experiments. Nuclei were isolated and processed as in dataset 1, but nuclei from all donors were pooled before loading into the tagmentation plates (5,000 nuclei per well), so barcodes were not linked to donors in advance. Libraries were prepared with the Single-cell DNA Methylation Kit (v1.1). Donor identity was assigned afterwards from genetic variants using cellsnp-lite and Vireo, with the previously called donor genotypes, which also flagged heterotypic doublets.

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Data policy of Data Access Committee of UZ Leuven

The procedure described below always applies to re-use transfer of genomic or genetic data that are in an external database. This external database can be the European Genome-phenome Archive (EGA). When a researcher receives requests for reuse of data, or when the database requires an intervention of a data access committee, the DAC will assess these requests for reuse and transfer of genomic or genetic data by third parties. Patient consent to research participation (ICF) and compatibility with new data processing should be checked. An application should be submitted at dac@uzleuven.be. So applications for re-use of genomic/genomic data go through the DAC. In the first place, DAC performs a front desk function here. An application form should be completed by the applicant. To begin with, the DAC employee then checks the ICF and its compatibility with further use. The DAC employee also ensures that a check is made with the competent services to ensure that there are no legal impediments. If i) there are possible commercial interests (commercial use of the data by the recipient or the requesting party is a commercial party), ii) the researcher does not agree, or iii) there is some ambiguity about potential risks of the data transfer (e.g. violation (GDPR) legislation, identity of recipient party, use of the data), the question is referred to the DAC advisory board.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000002212 Epigenetics

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
EGAF50001070044 fastq.gz 9.4 GB
EGAF50001070045 fastq.gz 8.5 GB
EGAF50001070046 fastq.gz 1.1 GB
EGAF50001070047 fastq.gz 1.1 GB
EGAF50001070048 fastq.gz 12.0 GB
EGAF50001070049 fastq.gz 1.4 GB
EGAF50001070050 fastq.gz 1.3 GB
EGAF50001070051 fastq.gz 10.9 GB
EGAF50001070052 fastq.gz 9.9 GB
EGAF50001070053 fastq.gz 1.1 GB
EGAF50001070054 fastq.gz 1.1 GB
EGAF50001070055 fastq.gz 8.9 GB
EGAF50001070056 fastq.gz 10.1 GB
EGAF50001070057 fastq.gz 1.3 GB
EGAF50001070058 fastq.gz 1.2 GB
EGAF50001070059 fastq.gz 11.2 GB
EGAF50001070060 fastq.gz 11.6 GB
EGAF50001070061 fastq.gz 1.3 GB
EGAF50001070062 fastq.gz 1.3 GB
EGAF50001070063 fastq.gz 10.5 GB
EGAF50001070064 fastq.gz 1.4 GB
EGAF50001070065 fastq.gz 1.3 GB
EGAF50001070066 fastq.gz 12.0 GB
EGAF50001070067 fastq.gz 10.9 GB
EGAF50001070068 fastq.gz 1.3 GB
EGAF50001070069 fastq.gz 1.3 GB
EGAF50001070070 fastq.gz 11.7 GB
EGAF50001070071 fastq.gz 10.6 GB
EGAF50001070072 fastq.gz 11.1 GB
EGAF50001070073 fastq.gz 1.5 GB
EGAF50001070074 fastq.gz 1.4 GB
EGAF50001070075 fastq.gz 12.3 GB
EGAF50001070076 fastq.gz 1.4 GB
EGAF50001070077 fastq.gz 1.6 GB
EGAF50001070078 fastq.gz 12.5 GB
EGAF50001070079 fastq.gz 11.3 GB
EGAF50001070080 fastq.gz 11.8 GB
EGAF50001070081 fastq.gz 1.4 GB
EGAF50001070082 fastq.gz 1.3 GB
EGAF50001070083 fastq.gz 10.6 GB
EGAF50001070084 fastq.gz 11.7 GB
EGAF50001070085 fastq.gz 1.3 GB
EGAF50001070086 fastq.gz 1.3 GB
EGAF50001070087 fastq.gz 10.5 GB
EGAF50001070088 fastq.gz 1.3 GB
EGAF50001070089 fastq.gz 1.3 GB
EGAF50001070090 fastq.gz 11.4 GB
EGAF50001070091 fastq.gz 10.3 GB
48 Files (292.9 GB)