3884 results for "RNA AND sequencing OR transcriptome"

in 7.44 milliseconds.

• Inference_of_B_cell_clonality_and_function_from_single_cell_RNA_seq_data

  cDNA libraries for RNA-seq will be prepared for sequencing. cDNA will be created following the Smart-Seq2 protocol, and a part of the cDNA from each cell will be used for preparation of RNA-seq libraries using the Nextera XT kit. The other part of the cDNA will be used as template for verification of the computationally recovered immunoglobulin sequences from the RNA-seq dataset by using a targeted PCR approach to generate a multiplexed sequencing library

  Study EGAS00001002963

• RCC Infiltrated Macrophages and Monocytes

  Tumor infiltrated Macrophages and Monocytes were sorted on Aria II (Becton Dickinson) into TRIzol LS and flash frozen. RNA was extracted with chloroform. Isopropanol and linear acrylamide were added, and the RNA was precipitated with 75% ethanol. Total RNA (0.649–1 ng) with RNA integrity numbers 6.8 to 10 underwent amplification using the SMART-Seq v4. Ultra Low Input RNA Kit (Clontech; cat. #63488). Amplified cDNA (15 ng) was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems, KK8504) using 8 cycles of PCR.

  Dataset EGAD00001009393

• Whole transcriptome sequencing of pheochromocytoma and paraganglioma arising from germline SDHB mutations

  Paired-end 150 basepair whole transcriptome sequencing of 91 SDHB-deficient non-metastatic and metastatic pheochromocytoma and paraganglioma. Samples were prepared using the NEB-Next directional RNA-Seq kit (NEB, USA) and underwent 150bp paired-end sequencing on the Illumina NovaSeq 6000 (Illumina, USA) according to manufacturer’s instructions. This dataset contains raw sequencing reads in FASTQ format.

  Dataset EGAD50000000504

• RNA-seq data from 7 CAF-S1 primary cell lines isolated from breast Cancer

  Dataset EGAD00001006144

• Transcriptomic profiling of lymphedema

  RNA-seq of skin from human subjects with and without lymphedema

  Dataset EGAD00001006246

• CRUK Accelerator: oesophageal adenocarcinoma whole exome and RNA-seq raw sequencing data

  Whole exome and RNASeq raw sequencing data for a cohort of 7 male patients with oesophageal adenocarcinoma. Median age at diagnosis was 68. Tumour tissue and PBMCs were used for whole exome sequencing and RNA sequencing. This data was generated as part of a study funded by a Cancer Research UK Centres Network Accelerator Award Grant (A21998).

  Dataset EGAD00001008489

• RNA sequencing data of pediatric B-other acute lymphoblastic leukemia (set 6)

  The dataset includes RNA sequencing FASTQ files from diagnostic tumor samples of three B-other ALL patients (ALLT-311, ALLK-131, ALLK-138). Novogene's internal method was used in directional library preparation including rRNA removal. The diagnostic tumor samples were sequenced using Illumina Novaseq X Plus, and the target sequencing depth was 21 Gb.

  Dataset EGAD50000002602

• Medulloblastoma dataset

  This dataset contains a tumor + normal DNA sequence data and tumor RNA seq data for a medulloblastoma patient.

  Dataset EGAD50000000384

• raw fastq file from 10x genomics sequencing

  RNA samples of bone marrow and cord blood were sequenced by 10x genomics platform.

  Dataset EGAD00001004200

• RNA-seq dataset for two NUP98-Rearranged Acute Myeloid Leukemia PDX samples

  This dataset includes RNA-seq of two patient-derived xenograft (PDX) samples with NUP98-Rearranged Acute Myeloid Leukemia. Bam files are provided for each sample.

  Dataset EGAD50000001564