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• A96186A

  Whole genome sequencing for single cells for library A96186A 850 samples; filetype=bam

  Dataset EGAD00001008249

• A96186C

  Whole genome sequencing for single cells for library A96186C 525 samples; filetype=bam

  Dataset EGAD00001008250

• A96199B

  Whole genome sequencing for single cells for library A96199B 1170 samples; filetype=bam

  Dataset EGAD00001008251

• A96201A

  Whole genome sequencing for single cells for library A96201A 536 samples; filetype=bam

  Dataset EGAD00001008252

• A96205A

  Whole genome sequencing for single cells for library A96205A 1907 samples; filetype=bam

  Dataset EGAD00001008253

• A96210C

  Whole genome sequencing for single cells for library A96210C 1191 samples; filetype=bam

  Dataset EGAD00001008254

• A96211C

  Whole genome sequencing for single cells for library A96211C 1397 samples; filetype=bam

  Dataset EGAD00001008255

• A96215A

  Whole genome sequencing for single cells for library A96215A 1312 samples; filetype=bam

  Dataset EGAD00001008256

• A96216A

  Whole genome sequencing for single cells for library A96216A 1001 samples; filetype=bam

  Dataset EGAD00001008257

• A96220B

  Whole genome sequencing for single cells for library A96220B 1267 samples; filetype=bam

  Dataset EGAD00001008258

• A96244A

  Whole genome sequencing for single cells for library A96244A 1374 samples; filetype=bam

  Dataset EGAD00001008259

• A98176A

  Whole genome sequencing for single cells for library A98176A 1003 samples; filetype=bam

  Dataset EGAD00001008260

• A98176B

  Whole genome sequencing for single cells for library A98176B 1389 samples; filetype=bam

  Dataset EGAD00001008261

• A98284A

  Whole genome sequencing for single cells for library A98284A 1265 samples; filetype=bam

  Dataset EGAD00001008262

• A98284B

  Whole genome sequencing for single cells for library A98284B 1582 samples; filetype=bam

  Dataset EGAD00001008263

• A98289B

  Whole genome sequencing for single cells for library A98289B 1032 samples; filetype=bam

  Dataset EGAD00001008264

• A98293B

  Whole genome sequencing for single cells for library A98293B 703 samples; filetype=bam

  Dataset EGAD00001008265

• Targeted de novo phasing and long-range assembly by template mutagenesis

  Long-range sequencing with low error rate has been challenging. Sequence assembly and phasing usually require a high-quality reference genome for mapping, so working on highly-variable genomic regions or regions with no reference genome information would be difficult. In this study, we describe novel bench protocols and algorithms to obtain ultra-low-error-rate haplotype-phased sequence assemblies of regions 10 KB in length using a short-read sequencing platform that simultaneously solves the above two problems. We accomplish this by imprinting each template strand from a target region with a dense and unique mutation pattern. The mutation process randomly and independently converts ~50% of cytosines to uracils. Short-read sequencing libraries are made from both mutated and unmutated templates. A conservative de Bruijn graph approach seeds an assembly of the mutated templates, which we then extend by mapping paired-end reads. We next partition the template assemblies into two or more haplotypes after using the unmutated sequence library to recover almost all of the mutated bases. The final haplotype is assembled and corrected for residual template mutations and PCR errors. We obtain per-base-error rates below 10 9. We apply this method to a human family, correctly assembling and phasing three genomic intervals, including the highly polymorphic HLA-B gene.

  Dataset EGAD00001008444

• Tumour sample for patient SA230

  Whole genome sequencing of tumour sample for triple negative breast cancer patient SA230

  Dataset EGAD00001009524

• Tumour sample for patient SA211

  Whole genome sequencing of tumour sample for triple negative breast cancer patient SA211

  Dataset EGAD00001009523

• Tumour sample for patient SA232

  Whole genome sequencing of tumour sample for triple negative breast cancer patient SA232

  Dataset EGAD00001009522

• Tumour sample for patient SA597

  Whole genome sequencing of tumour sample for triple negative breast cancer patient SA597

  Dataset EGAD00001009521

• Tumour sample for patient SA296

  Whole genome sequencing of tumour sample for triple negative breast cancer patient SA296

  Dataset EGAD00001009520

• Tumour sample for patient SA420

  Whole genome sequencing of tumour sample for triple negative breast cancer patient SA420

  Dataset EGAD00001009519

• Tumour sample for patient SA409

  Whole genome sequencing of tumour sample for triple negative breast cancer patient SA409

  Dataset EGAD00001009518