Need Help?

Reproducibility of variant calls in replicate next generation sequencing experiments

In this study, we examined the reproducibility of nucleotide variant calls in replicate sequencing experiments of the same genomic DNA. We performed targeted sequencing of all known human protein kinase genes (kinome) (~3.3 Mb) using the SOLiD v4 platform. Seventeen breast cancer samples were sequenced in duplicate (n=14) or triplicate (n=3) to assess concordance of all calls and single nucleotide variant (SNV) calls.

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID Description Technology Samples
EGAD00001001209 AB SOLiD 4 System 37
Publications Citations
Reproducibility of Variant Calls in Replicate Next Generation Sequencing Experiments.
PLoS One 10: 2015 e0119230