Study
Comparison of EGF and PDGF driven glioblastomas.
Study ID | Alternative Stable ID | Type |
---|---|---|
EGAS00001001900 | Other |
Study Description
Glioblastoma multiforme (GBM) is the most common and aggressive type of primary brain tumor. Epidermal growth-factor (EGF) and platelet-derived growth-factor (PDGF) receptors are frequently amplified and/or possess gain-of-function mutations in these tumors. Despite years of research into tyrosine-kinase inhibitors, clinical trials have shown disappointing efficacy. One of the underlying reasons is intra-tumor heterogeneity. To assess the effect of intra-tumor heterogeneity on gene expression, we derived a general approach to map single-cell expression profiles to sequentially-acquired mutations identified from exome sequencing. Using 288 single cells, we constructed high-resolution phylogenies of EGF-driven and PDGF-driven GBMs, modeling transcriptional kinetics during tumor evolution. A deletion in a PDGF-receptor domain involved in dimerization correlated to an up-regulation of the PI3K/AKT pathway in a proneural GBM, and ectopic expression of this mutant PDGFRA in vitro enhanced proliferation and invasiveness. Descending the phylogenetic tree of the PDGFR-driven tumor ... (Show More)
Study Datasets 7 datasets.
Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data
Dataset ID | Description | Technology | Samples |
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EGAD00001002269 |
We expressed PDGFRAmut, wild-type PDGFRA and a GFP control from lentivirus, in two primary GBM patient-derived cell lines that we had cultured as monolayers.
|
Illumina HiSeq 4000 | 1 |
EGAD00001002270 |
We collected fresh tissue from an untreated GBM (SF10282) directly from the operating
room and subjected the biopsy to single-cell RNA-seq with the fluidigm C1 machine, resulting in sequencing libraries from 96 individual cells.
|
Illumina HiSeq 2500 | 1 |
EGAD00001002271 |
We collected fresh tissue from an untreated GBM (SF10345) directly from the operating
room and subjected the biopsy to single-cell RNA-seq with the fluidigm C1 machine, resulting in sequencing libraries from 96 individual cells.
|
Illumina HiSeq 2500 | 1 |
EGAD00001002272 |
We collected fresh tissue from an untreated GBM (SF10360) directly from the operating
room and subjected the biopsy to single-cell RNA-seq with the fluidigm C1 machine, resulting in sequencing libraries from 96 individual cells.
|
Illumina HiSeq 2500 | 1 |
EGAD00001002273 |
We performed bulk exome-seq on a primary GBM and a blood sample from SF10345
|
Illumina HiSeq 2500 | 1 |
EGAD00001002274 |
We performed bulk exome-seq on a primary GBM and a blood sample from SF10360
|
Illumina HiSeq 2500 | 1 |
EGAD00001002275 |
We performed bulk exome-seq on a primary GBM and a blood sample from SF10282
|
Illumina HiSeq 2500 | 1 |
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