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We will take a bone marrow aspirate and peripheral blood samples from a healthy patient aged around 60, and use flow cytometry to isolate 100 HSCs, 50 MEPs, and 50 GMPs. We will grow these up into colonies, then whole genome sequence each colony. Somatic mutations will act as a unique barcode for each clone. We will then design a panel for targeted resequencing of the mutations that we find. It will then be possible to look for these mutations in the peripheral blood over several years, to see the dynamics of how HSCs contribute to the peripheral blood in health.

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID Description Technology Samples
EGAD00001004086 HiSeq X Ten Illumina HiSeq 2500 207
EGAD00001006595 HiSeq X Ten Illumina NovaSeq 6000 13
EGAD00001008107 HiSeq X Ten 717
Publications Citations
Diverse mutational landscapes in human lymphocytes.
Nature 608: 2022 724-732
Measures of genetic diversification in somatic tissues at bulk and single-cell resolution.
Elife 12: 2024 RP89780