Study
Single Cell RNAseq at various stages of HiPSCs differentiating toward definitive endoderm and endoderm derived lineages
Study ID | Alternative Stable ID | Type |
---|---|---|
EGAS00001002278 | Transcriptome Analysis |
Study Description
When comparing the differentiation capacities of pluripotent stem cell lines that have different
genetic backgrounds, batch to batch experimental variablility poses a significant challenge,
especially when trying to identify smaller effects. One way to address this issue is to
differentiate several different lines in the same culture dish, thereby elimating experimental
variation. In addition, it allows researchers to analyze many more lines with less experiments.
Parallel single cell RNA-Seq exploits that individual cells are tagged and hence each cell can
be reliably assigned to the donor of origin based on the genetic variants it contains. In
addition, analyzing the genetic signature of single cells within a differentiating population can
reveal differentation stages that are not easily detected in bulk RNAseq data.
This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Study Datasets 1 dataset.
Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data
Dataset ID | Description | Technology | Samples |
---|---|---|---|
EGAD00001005741 |
When comparing the differentiation capacities of pluripotent stem cell lines that have different
genetic backgrounds, batch to batch experimental variablility poses a significant challenge,
especially when trying to identify smaller effects. One way to address this issue is to
differentiate several different lines in the same culture dish, thereby elimating experimental
variation. In addition, it allows researchers to analyze many more lines with less experiments.
Parallel single cell RNA-Seq ... (Show More)
|
Illumina HiSeq 2500 | 13433 |
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