Dataset

Single Cell RNAseq at various stages of HiPSCs differentiating toward definitive endoderm and endoderm derived lineages

Dataset ID Technology Samples
EGAD00001005741 Illumina HiSeq 2500 13433

Dataset Description

When comparing the differentiation capacities of pluripotent stem cell lines that have different
genetic backgrounds, batch to batch experimental variablility poses a significant challenge,
especially when trying to identify smaller effects. One way to address this issue is to
differentiate several different lines in the same culture dish, thereby elimating experimental
variation. In addition, it allows researchers to analyze many more lines with less experiments.
Parallel single cell RNA-Seq exploits that individual cells are tagged and hence each cell can
be reliably assigned to the donor of origin based on the genetic variants it contains. In
addition, analyzing the genetic signature of single cells within a differentiating population can
reveal differentation stages that are not easily detected in bulk RNAseq data.
This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

Who controls access to this dataset

For each dataset that requires controlled access, there is a corresponding Data Access Committee (DAC) who determine access permissions. Access to actual data files is not managed by the EGA. If you need to request access to this data set, please contact:

Wellcome Trust Sanger Institute
Contact person: Data Sharing
Email: datasharing [at] sanger [dot] ac [dot] uk
Access information: https://www.sanger.ac.uk/legal/DAA/MasterController
More details: EGAC00001000205

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