Whole genome sequencing identified biomarker of response to PD1 blockade in Natural-killer/T-cell lymphoma

WGS data of 13 and TCS data of 8 NKTCL patients. Significant association of PD-L1 rearrangements with objective response to anti-PD1 (pembrolizumab).

1 Dataset

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Dataset ID	Description	Technology	Samples
EGAD00001005230	Whole-transcriptome sequencing (WTS) of 36 samples from patients diagnosed with NKTL. Total RNA from snap frozen EITL tumor samples was extracted using TRIzol (Invitrogen) and purified with RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions. The integrity of RNA was determined by electrophoresis using 2100 Bioanalyzer (Agilent Technologies). 500 ng of total RNA was reverse transcribed with iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA). Quantification was performed using SsoFast EvaGreen Supermix and CFX96 Real-Time PCR System (both Bio-Rad). Sequencing libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero (Illumina) and WTS was performed on HiSeq 2500 and HiSeq 3000 (Illumina) with 2x101 bp and 2x151 bp read length, respectively.	HiSeq X Ten Illumina HiSeq 2000 Illumina HiSeq 2500	36